公开(公告)号：US5143852A

公开(公告)日：1992-09-01

申请号：US583046

申请日：1990-09-14

申请人： Gunars E. Valkirs ,  Kenneth F. Buechler

发明人： Gunars E. Valkirs ,  Kenneth F. Buechler

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/541 ,  G01N33/543

CPC分类号： G01N33/54306 ,  G01N33/53 ,  G01N33/5306 ,  G01N33/541 ,  G01N33/54393 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/822 ,  Y10S436/824

摘要： Means for the detection of free ligand analogue conjugate in fluids from competitive ligand-receptor assay processes. Ligand analogue antibodies that bind the ligand analogue conjugate with substantially greater affinity than their affinity for target ligand are selected and used in competitive ligand-receptor assay processes to bind the free fraction of the ligand analogue conjugate. This means permits the detection of the free fraction of ligand analogue conjugate even in the presence of substantially higher concentrations of free target ligand. For the purposes of the present invention, ligand analogue antibodies are antibodies that exhibit at least 100.times.greater affinity for the ligand analogue conjugate compared to their affinity for the target ligand.

公开(公告)号：US20110065210A1

公开(公告)日：2011-03-17

申请号：US12874859

申请日：2010-09-02

申请人： Jeffrey R. Dahlen ,  Kenneth F. Buechler ,  Gunars E. Valkirs

发明人： Jeffrey R. Dahlen ,  Kenneth F. Buechler ,  Gunars E. Valkirs

IPC分类号： G01N33/566

CPC分类号： G01N33/6887 ,  G01N33/6893 ,  G01N2333/58 ,  G01N2800/324

摘要： The present invention relates to materials and procedures for evaluating the prognosis of patients suffering from acute coronary syndromes. In particular, the level of BNP, or a marker related to BNP, in a patient sample, alone or in combination with one or more other prognostic markers, provides prognostic information useful for predicting near-term morbidity and/or mortality across the entire spectrum of acute coronary syndromes, including unstable angina, non-ST-elevation non-Q wave myocardial infarction, ST-elevation non-Q wave MI, and transmural (Q-wave) MI.

摘要翻译： 本发明涉及用于评估患有急性冠状动脉综合征的患者的预后的材料和程序。 特别地，在患者样品中单独或与一种或多种其他预后标志物组合的BNP或与BNP相关的标志物的水平提供了可用于预测整个频谱中的近期发病率和/或死亡率的预后信息 急性冠状动脉综合征，包括不稳定型心绞痛，非ST段抬高型非Q波心肌梗死，ST段抬高型非Q波心肌梗死和透壁（Q波）MI。

公开(公告)号：US5985579A

公开(公告)日：1999-11-16

申请号：US458901

申请日：1995-06-02

申请人： Kenneth F. Buechler ,  Gunars E. Valkirs

发明人： Kenneth F. Buechler ,  Gunars E. Valkirs

IPC分类号： G01N33/53 ,  G01N33/538 ,  G01N33/541 ,  G01N33/543 ,  G01N33/564 ,  G01N33/566

CPC分类号： G01N33/54306 ,  G01N33/5306 ,  G01N33/541 ,  G01N33/54393 ,  Y10S435/97 ,  Y10S435/971 ,  Y10S435/975 ,  Y10S436/807

摘要： Methods and test devices for detecting the presence or amount of target ligand in non-competitive sandwich ligand-receptor assay processes. Antibodies which bind to the complex of ligand receptor and target ligand but do not bind significantly to the ligand receptor and which bind the target ligand with substantially less affinity than the complex are taught and their uses described. These assays can be used to eliminate the "hook" effect in non-competitive sandwich assays. Furthermore, the antibodies are selected and assay methods described so that, as a result of the assay process, no detectable response is observed due to the binding of antibody and ligand receptor in the absence of target ligand.

摘要翻译： 用于检测非竞争性三明治配体 - 受体测定方法中靶配体的存在或量的方法和测试装置。 教导了与配体受体和靶配体的配合物结合但不与配体受体显着结合并以与复合物相近的亲和力结合靶配体的抗体，并描述了它们的用途。 这些测定可用于消除非竞争性夹心测定中的“钩”效应。 此外，选择抗体并描述测定方法，使得作为测定方法的结果，由于在不存在靶配体的情况下抗体和配体受体的结合，观察不到可检测的反应。

公开(公告)号：US07632647B2

公开(公告)日：2009-12-15

申请号：US09835298

申请日：2001-04-13

申请人： Jeffrey R. Dahlen ,  Kenneth F. Buechler ,  Gunars E. Valkirs

发明人： Jeffrey R. Dahlen ,  Kenneth F. Buechler ,  Gunars E. Valkirs

IPC分类号： G01N33/53

CPC分类号： G01N33/6887 ,  G01N33/6893 ,  G01N2333/58 ,  G01N2800/324

摘要： The present invention relates to materials and procedures for evaluating the prognosis of patients suffering from acute coronary syndromes. In particular, the level of BNP, or a marker related to BNP, in a patient sample, alone or in combination with one or more other prognostic markers, provides prognostic information useful for predicting near-term morbidity and/or mortality across the entire spectrum of acute coronary syndromes, including unstable angina, non-ST-elevation non-Q wave myocardial infarction, ST-elevation non-Q wave MI, and transmural (Q-wave) MI.

摘要翻译： 本发明涉及用于评估患有急性冠状动脉综合征的患者的预后的材料和程序。 特别地，在患者样品中单独或与一种或多种其他预后标志物组合的BNP或与BNP相关的标志物的水平提供了可用于预测整个频谱中的近期发病率和/或死亡率的预后信息 急性冠状动脉综合征，包括不稳定型心绞痛，非ST段抬高型非Q波心肌梗死，ST段抬高型非Q波心肌梗死和透壁（Q波）MI。

公开(公告)号：US07427490B2

公开(公告)日：2008-09-23

申请号：US10225082

申请日：2002-08-20

申请人： Gunars E. Valkirs ,  Jeffrey R. Dahlen ,  Howard J. Kirchick ,  Kenneth F. Buechler

发明人： Gunars E. Valkirs ,  Jeffrey R. Dahlen ,  Howard J. Kirchick ,  Kenneth F. Buechler

IPC分类号： G01N33/00

CPC分类号： G01N33/6893 ,  G01N33/573 ,  G01N2333/96486 ,  G01N2800/2871 ,  Y10S436/811

摘要： The present invention relates to methods for the diagnosis and evaluation of stroke and transient ischemic attacks. In a particular aspect, patient samples are analyzed for the presence or amount of a panel of markers comprising one or more specific markers for cerebral injury and one or more non-specific markers for cerebral injury. In an alternative aspect, samples are analyzed for B-type natriuretic peptide. A variety of markers are disclosed for assembling a panel for such diagnosis and evaluation. In various aspects, the invention provides methods for early detection and differentiation of stroke types and transient ischemic attacks, for determining the prognosis of a patient presenting with stroke symptoms, and identifying a patient at risk for cerebral vasospasm. Invention methods provide rapid, sensitive and specific assays to greatly increase the number of patients that can receive beneficial stroke treatment and therapy, and reduce the costs associated with incorrect stroke diagnosis.

摘要翻译： 本发明涉及中风和短暂性脑缺血发作的诊断和评估方法。 在特定方面，分析患者样品的一组标志物的存在或量，其包含一种或多种用于脑损伤的特异性标志物和一种或多种用于脑损伤的非特异性标志物。 在另一方面，分析B型利尿钠肽的样品。 公开了用于组装用于这种诊断和评估的面板的各种标记。 在各个方面，本发明提供了用于早期检测和分化卒中类型和短暂性脑缺血发作的方法，用于确定患有中风症状的患者的预后，以及鉴定患有脑血管痉挛风险的患者。 本发明方法提供快速，灵敏和特异性的测定以大大增加可以接受有益的中风治疗和治疗的患者的数量，并且降低与不正确的中风诊断相关的成本。

公开(公告)号：US07052858B2

公开(公告)日：2006-05-30

申请号：US09877933

申请日：2001-06-07

申请人： Jeff Gray ,  Gunars E. Valkirs ,  Joe Buechler

发明人： Jeff Gray ,  Gunars E. Valkirs ,  Joe Buechler

IPC分类号： G01N33/53 ,  G01N33/567 ,  G01N33/537 ,  G01N33/543 ,  C12M1/36

CPC分类号： C07K14/44 ,  G01N33/56905 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/81

摘要： This invention provides a novel Cryptosporidium parvum protein disulfide isomerase polypeptide, and nucleic acids that encode this polypeptide. The invention also provides methods, reagents, and kits that are useful for diagnosing infection by Cryptosporidium parvum. The methods are based on the discovery of binding agents, including recombinant polyclonal antibodies, that bind to the protein disulfide isomerase polypeptide of C. parvum.

摘要翻译： 本发明提供了一种新型隐孢子虫蛋白二硫键异构酶多肽，以及编码该多肽的核酸。 本发明还提供了可用于诊断隐孢子虫感染的方法，试剂和试剂盒。 该方法是基于结合试剂（包括重组多克隆抗体）的发现，其结合小曲霉的蛋白质二硫键异构酶多肽。

公开(公告)号：US06302919B1

公开(公告)日：2001-10-16

申请号：US09358130

申请日：1999-07-20

申请人： Brian Chambers ,  Adam Sampson ,  Matt McDermott ,  Byron Knight ,  Gunars E. Valkirs ,  Howard J. Kirchick

发明人： Brian Chambers ,  Adam Sampson ,  Matt McDermott ,  Byron Knight ,  Gunars E. Valkirs ,  Howard J. Kirchick

IPC分类号： B01D2126

CPC分类号： G01N1/4077 ,  B01L3/5021

摘要： A filtration device, apparatus, and method of filtration useful for filtering liquid samples are disclosed wherein filtration occurs opposite the direction of centrifugal force. The filtration device is placed within a container that contains the liquid sample to be filtered. Together, the filtration device and the container comprise the filtration apparatus. Centrifugation forces the liquid sample present in the container to enter the filtration device, thereby effecting filtration. The vertical position of the filtration device is fixed within this container during filtration.

摘要翻译： 公开了用于过滤液体样品的过滤装置，装置和过滤方法，其中过滤与离心力方向相反。 将过滤装置放置在容纳要过滤的液体样品的容器内。 过滤装置和容器一起包括过滤装置。 离心迫使存在于容器中的液体样品进入过滤装置，从而进行过滤。 在过滤期间，过滤装置的垂直位置固定在该容器内。

公开(公告)号：US06207395B1

公开(公告)日：2001-03-27

申请号：US09158347

申请日：1998-09-21

申请人： Gunars E. Valkirs ,  Joe Buechler ,  Jeff Gray

发明人： Gunars E. Valkirs ,  Joe Buechler ,  Jeff Gray

IPC分类号： G01N3353

CPC分类号： G01N33/56905 ,  C07K16/20 ,  Y10S435/947

摘要： This invention provides methods, reagents, and kits that are useful for diagnosing infection by E. histolytica. The methods are based on the discovery of binding agents, including recombinant polyclonal antibodies, that bind to the 29 kDa antigen of E. histolytica.

公开(公告)号：US06908739B2

公开(公告)日：2005-06-21

申请号：US10155163

申请日：2002-05-23

申请人： Joe Buechler ,  Shanthi Govindaraj ,  Jeff Gray ,  Gunars E. Valkirs

发明人： Joe Buechler ,  Shanthi Govindaraj ,  Jeff Gray ,  Gunars E. Valkirs

IPC分类号： G01N33/569 ,  G01N33/53 ,  A61K39/02 ,  A61K39/395 ,  G01N33/567

CPC分类号： G01N33/56905

摘要： This invention provides methods, reagents, and kits that are useful for diagnosing infection by Giardia lamblia. The methods are based on the discovery of binding agents, including recombinant polyclonal antibodies, that bind to the α-1-giardin antigen of G. lamblia.

摘要翻译： 本发明提供了可用于诊断贾第鞭毛虫感染的方法，试剂和试剂盒。 所述方法基于结合羊链球菌的α-1-吉达尔抗原的结合剂（包括重组多克隆抗体）的发现。

公开(公告)号：US06348318B1

公开(公告)日：2002-02-19

申请号：US09054918

申请日：1998-04-03

申请人： Gunars E. Valkirs

发明人： Gunars E. Valkirs

IPC分类号： G01N33543

CPC分类号： C07K16/1282 ,  G01N33/54326 ,  G01N35/0098 ,  G01N2333/33 ,  G01N2400/50 ,  Y10S435/975 ,  Y10S436/806 ,  Y10S436/824

摘要： This invention provides methods, compositions and kits for concentrating target ligands, including microorganisms, from samples, including biological samples. The methods involve the use of magnetic particles to concentrate the target analytes. Also provided are methods, compositions and kits for detecting the presence of target ligands in samples.

摘要翻译： 本发明提供了用于从包括生物样品的样品中浓缩目标配体（包括微生物）的方法，组合物和试剂盒。 这些方法涉及使用磁性颗粒来浓缩目标分析物。 还提供了用于检测样品中靶配体存在的方法，组合物和试剂盒。