公开(公告)号：US20140288300A1

公开(公告)日：2014-09-25

申请号：US13936768

申请日：2013-07-08

Applicant: Korea Institute of Science and Technology

Inventor： Kwang Meyung KIM ,  Ick Chan KWON ,  Sang Yoon KIM ,  Ju Hee RYU ,  Eun Sung JUN ,  In San KIM

IPC: C07D499/78 ,  C07D409/14 ,  C07D498/08 ,  C07D209/62

CPC classification number: C07D499/78 ,  C07D209/62 ,  C07D409/14 ,  C07D498/08

Abstract: Disclosed is a drug-fluorophore complex for specific detection of tumor cells. Specifically, the drug-fluorophore complex includes a tumor cell-targeting drug penetrating tumor cells and non-tumor cells at different rates or levels, and a fluorescent substance chemically bonded to the tumor cell-targeting drug. The drug-fluorophore complex enables specific imaging of tumor cells only with high accuracy in a very simple manner without causing cytotoxicity.

Abstract translation: 公开了用于特异性检测肿瘤细胞的药物 - 荧光团复合物。 具体地，药物 - 荧光团复合物包括以不同速率或水平渗透肿瘤细胞和非肿瘤细胞的肿瘤细胞靶向药物和与肿瘤细胞靶向药物化学键合的荧光物质。 药物荧光团复合物能够以非常简单的方式以高精度进行肿瘤细胞的特异性成像，而不引起细胞毒性。

公开(公告)号：US20200087705A1

公开(公告)日：2020-03-19

申请号：US16568363

申请日：2019-09-12

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION

Inventor： Ick Chan KWON ,  Ju Hee RYU ,  Young-Ji KO ,  Hye-sun KIM ,  Nam-hyuk CHO ,  Eun-Jeong YANG ,  Jae Won LEE

IPC: C12Q1/37 ,  G01N33/573

Abstract: A probe for measuring the activity of caspase-1 according to the present disclosure can specifically image cells or tissues where inflammatory response is induced because it is cleaved by reacting specifically with the active caspase-1 enzyme in vivo and in vitro and re-emits fluorescence. The probe for measuring the activity of caspase-1 can be used for various purposes, such as for imaging of cells or tissues where inflammatory response is induced, as a drug carrier, for screening of a drug inhibiting inflammatory response, etc.The probe for measuring the activity of caspase-1 is applicable both in vivo and in vitro, and can be used for various applications such as high-throughput screening for new drug development, early diagnosis of diseases, etc.

公开(公告)号：US20200071698A1

公开(公告)日：2020-03-05

申请号：US16425849

申请日：2019-05-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Hwa KIM ,  Ick Chan KWON ,  Kwangmeyung KIM ,  Hong Yeol YOON ,  Gi-Jung KWAK ,  Juho PARK

IPC: C12N15/113 ,  A61P35/00

Abstract: The present invention relates to a novel anti-miRNA single-stranded nucleic acid maleimide derivative, which comprises an anti-miRNA single-stranded nucleic acid having a nucleic acid sequence complementary to a nucleic acid sequence of an miRNA. Further, the present invention provides an anti-miRNA single-stranded nucleic acid-serum albumin conjugate in which serum albumin is covalently bonded to the anti-miRNA single-stranded nucleic acid maleimide derivative via the maleimide group.

公开(公告)号：US20170216460A1

公开(公告)日：2017-08-03

申请号：US15169493

申请日：2016-05-31

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kwangmeyung KIM ,  Ju Hee RYU ,  Ick Chan KWON ,  Man Kyu SHIM ,  Hong Yeol YOON

IPC: A61K49/00 ,  G01N33/58 ,  G01N33/574 ,  C07K9/00

CPC classification number: A61K49/0056 ,  A61K47/555 ,  A61K49/0032 ,  A61K49/0052 ,  C07K5/1016 ,  C07K5/1019 ,  C07K9/001 ,  G01N33/57496 ,  G01N33/581 ,  G01N2333/96466

Abstract: The present disclosure relates to a glycopeptide targeting cancer cells and a contrast agent kit containing the same. The glycopeptide is one wherein an azide reporting monosaccharide is bound to a substrate peptide. As the substrate peptide is specifically cleaved by cathepsin B in cancer cells, an azide reporting monosaccharide is expressed onto the cell surface via metabolic glycoengineering, thereby providing a target for action as a contrast agent. Accordingly, because the azide is exposed to the cell surface only by cathepsin B, as it is specifically expressed in cancer cells, in particular in metastatic cancer cells, while it is limitedly expressed in normal cells and is hardly excreted out the cells, the cancer cells can be selectively imaged by an azide-specific contrast agent.

公开(公告)号：US20140147493A1

公开(公告)日：2014-05-29

申请号：US14071803

申请日：2013-11-05

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ick Chan KWON ,  Kwang Meyung KIM ,  Sun Hwa KIM ,  In-Cheol SUN ,  Jin Hee NA ,  Sangmin LEE ,  Ju Hee RYU

IPC: A61K48/00 ,  A61K9/127

CPC classification number: A61K9/127 ,  C12N15/88 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2563/161

Abstract: Disclosed is an efficient carrier for gene delivery to cells based on PCR. The PCR-based gene delivery carrier includes: a shell composed of neutral liposomes; and template DNA and PCR components including polymerase, dNTPs and primers for amplification of the template DNA by PCR, within an inner space defined by the shell. The gene delivery carrier enhances the gene loading efficiency of neutral liposomes without cytotoxicity. Further disclosed is a method for preparing the gene delivery carrier.

Abstract translation: 公开了基于PCR的基因递送至细胞的有效载体。 基于PCR的基因递送载体包括：由中性脂质体组成的壳; 和模板DNA和PCR组分，包括聚合酶，dNTP和引物，用于通过PCR扩增模板DNA，在由壳定义的内部空间内。 基因递送载体增强了中性脂质体的基因负载效率，而没有细胞毒性。 进一步公开的是制备基因递送载体的方法。