公开(公告)号：US20130251784A1

公开(公告)日：2013-09-26

申请号：US13774247

申请日：2013-02-22

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kwang Meyung KIM ,  Ick Chan KWON ,  Kuiwon CHOI ,  Heebeom KOO ,  Sang-min LEE ,  Inchan YOUN

IPC: A61K47/26 ,  A61K47/34

CPC classification number: A61K47/26 ,  A61K47/34 ,  A61K47/549 ,  A61K47/555 ,  A61K47/6911 ,  A61K49/0032 ,  A61K49/0052

Abstract: The present disclosure relates to a method for in vivo targeting of a nanoparticle via bioorthogonal copper-free click chemistry, more particularly to a method for in vivo targeting of a nanoparticle, including: injecting a precursor capable of being metabolically engineered in vivo when injected into a living system and having a first bioorthogonal functional group into the living system; and injecting a nanoparticle having a second bioorthogonal functional group which can perform a bioorthogonal copper-free click reaction with the first bioorthogonal functional group attached thereto into the living system.In accordance with the present disclosure, accumulation of nanoparticles at a target site in a living system can be increased remarkably and the biodistribution of the nanoparticles can be controlled since the nanoparticles bound to a cell surface are taken up into the cell with time.

Abstract translation: 本公开内容涉及一种通过生物正交无铜点击化学体内靶向纳米颗粒的方法，更具体地涉及纳米颗粒的体内靶向方法，包括：注射能够在注射入体内时被代谢工程化的前体 生活系统，并具有第一生物正交功能组进入生活系统; 并且将具有第二生物正交官能团的纳米颗粒注射到与生物体系附着的第一生物正交官能团进行生物正交无铜点击反应。 根据本公开，可以显着增加生物体系中靶位点上的纳米颗粒的积累，并且可以控制纳米颗粒的生物分布，因为结合到细胞表面的纳米颗粒随时间被吸收到细胞中。

公开(公告)号：US20150038690A1

公开(公告)日：2015-02-05

申请号：US14340845

申请日：2014-07-25

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kwang Meyung KIM ,  Sun Hwa KIM ,  Ick Chan KWON ,  Ji Young YHEE ,  Sojin LEE

IPC: A61K47/48 ,  A61K31/7088

CPC classification number: A61K31/7088 ,  A61K31/713 ,  A61K47/6435 ,  A61K47/6903 ,  A61K47/6929

Abstract: Disclosed is a gelatin-based nanoparticle complex for tumor-targeted delivery of siRNA for specific gene silencing in tumor cells. The gelatin-based nanoparticle complex includes: poly-siRNA chains whose ends are modified with thiol groups; and thiolated gelatin bound to the poly-siRNA chains through disulfide crosslinking and charge interactions. The gelatin-based nanoparticle complex is not degraded in the bloodstream and can be efficiently absorbed into tumor cells without cytotoxicity. The delivered siRNA can effectively silence target gene expression. Also disclosed is a method for preparing the gelatin-based nanoparticle complex.

Abstract translation: 公开了一种用于肿瘤靶向递送siRNA用于肿瘤细胞中特异性基因沉默的明胶基纳米颗粒复合物。 基于明胶的纳米颗粒复合物包括：末端用巯基修饰的多siRNA链; 并通过二硫键交联和电荷相互作用将与siRNA结合的多聚siRNA链结合在一起。 基于明胶的纳米颗粒复合物在血液中不降解，并且可以有效地吸收到肿瘤细胞中而没有细胞毒性。 递送的siRNA可以有效地沉默靶基因表达。 还公开了一种制备明胶基纳米颗粒复合物的方法。

公开(公告)号：US20140288300A1

公开(公告)日：2014-09-25

申请号：US13936768

申请日：2013-07-08

Applicant: Korea Institute of Science and Technology

Inventor： Kwang Meyung KIM ,  Ick Chan KWON ,  Sang Yoon KIM ,  Ju Hee RYU ,  Eun Sung JUN ,  In San KIM

IPC: C07D499/78 ,  C07D409/14 ,  C07D498/08 ,  C07D209/62

CPC classification number: C07D499/78 ,  C07D209/62 ,  C07D409/14 ,  C07D498/08

Abstract: Disclosed is a drug-fluorophore complex for specific detection of tumor cells. Specifically, the drug-fluorophore complex includes a tumor cell-targeting drug penetrating tumor cells and non-tumor cells at different rates or levels, and a fluorescent substance chemically bonded to the tumor cell-targeting drug. The drug-fluorophore complex enables specific imaging of tumor cells only with high accuracy in a very simple manner without causing cytotoxicity.

Abstract translation: 公开了用于特异性检测肿瘤细胞的药物 - 荧光团复合物。 具体地，药物 - 荧光团复合物包括以不同速率或水平渗透肿瘤细胞和非肿瘤细胞的肿瘤细胞靶向药物和与肿瘤细胞靶向药物化学键合的荧光物质。 药物荧光团复合物能够以非常简单的方式以高精度进行肿瘤细胞的特异性成像，而不引起细胞毒性。

公开(公告)号：US20140147493A1

公开(公告)日：2014-05-29

申请号：US14071803

申请日：2013-11-05

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ick Chan KWON ,  Kwang Meyung KIM ,  Sun Hwa KIM ,  In-Cheol SUN ,  Jin Hee NA ,  Sangmin LEE ,  Ju Hee RYU

IPC: A61K48/00 ,  A61K9/127

CPC classification number: A61K9/127 ,  C12N15/88 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2563/161

Abstract: Disclosed is an efficient carrier for gene delivery to cells based on PCR. The PCR-based gene delivery carrier includes: a shell composed of neutral liposomes; and template DNA and PCR components including polymerase, dNTPs and primers for amplification of the template DNA by PCR, within an inner space defined by the shell. The gene delivery carrier enhances the gene loading efficiency of neutral liposomes without cytotoxicity. Further disclosed is a method for preparing the gene delivery carrier.

Abstract translation: 公开了基于PCR的基因递送至细胞的有效载体。 基于PCR的基因递送载体包括：由中性脂质体组成的壳; 和模板DNA和PCR组分，包括聚合酶，dNTP和引物，用于通过PCR扩增模板DNA，在由壳定义的内部空间内。 基因递送载体增强了中性脂质体的基因负载效率，而没有细胞毒性。 进一步公开的是制备基因递送载体的方法。