公开(公告)号：US20220119873A1

公开(公告)日：2022-04-21

申请号：US17517012

申请日：2021-11-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Adam BROOMER ,  Kelly LI ,  Andreas TOBLER ,  Caifu CHEN ,  David KEYS

IPC: C12Q1/6844

Abstract: This disclosure relates to methods and kits for karyotyping in which chromosomes are interrogated by amplifying loci that are not within copy number variable regions thereof.

公开(公告)号：US20160076090A1

公开(公告)日：2016-03-17

申请号：US14856448

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20160040256A1

公开(公告)日：2016-02-11

申请号：US14879156

申请日：2015-10-09

Applicant: Life Technologies Corporation

Inventor： Caifu CHEN ,  Ruoying Tan

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C07H21/04 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).