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公开(公告)号：US20180291339A1
公开(公告)日：2018-10-11
申请号：US16015748
申请日：2018-06-22
发明人： Lorenz Studer , Faranak Fattahi
IPC分类号： C12N5/0793 , A61K35/30
摘要： The presently disclosed subject matter provides for in vitro methods of inducing differentiation of stem cells into enteric neural crest lineage cells, and enteric neural crest lineage cells by such methods. The presently disclosed subject matter also provides for uses of such enteric neural crest lineage cells for preventing and/or treating enteric nervous system disorders (e.g, Hirschsprung's disease), and for screening compounds suitable for preventing and/or treating enteric nervous system disorders (e.g., Hirschsprung's disease).
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公开(公告)号：US20180237748A9
公开(公告)日：2018-08-23
申请号：US14168835
申请日：2014-01-30
发明人： Stuart Chambers , Lorenz Studer
IPC分类号： C12N5/0793
CPC分类号： C12N5/0619 , C12N2500/90 , C12N2501/15 , C12N2501/155 , C12N2501/41 , C12N2501/415 , C12N2501/60 , C12N2501/998 , C12N2506/02 , C12N2506/45
摘要： The present invention relates generally to the field of cell biology of stem cells, more specifically the directed differentiation of pluripotent or multipotent stem cells, including human embryonic stem cells (hESC), somatic stem cells, and induced human pluripotent stem cells (hiPSC) using novel culture conditions. Specifically, methods are provided for obtaining neural tissue, floor plate cells, and placode including induction of neural plate development in hESCs for obtaining midbrain dopamine (DA) neurons, motorneurons, and sensory neurons. Further, neural plate tissue obtained using methods of the present inventions are contemplated for use in co-cultures with other tissues as inducers for shifting differentiation pathways, i.e. patterning.
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公开(公告)号：US09963674B2
公开(公告)日：2018-05-08
申请号：US14884503
申请日：2015-10-15
发明人： Lorenz Studer , Justine D. Miller
CPC分类号： C12N5/0618 , C12N5/0696 , C12N2501/40 , C12N2501/998 , C12N2503/02 , C12N2506/1307 , G01N33/502 , G01N33/5023 , G01N33/5058 , G01N33/5073
摘要： Provided are age-modified cells and method for making age modified cells using progerin or a progerin-like protein. The aging and/or maturation process can be accelerated and controlled for young and/or immature cells, such as a somatic cell, a stem cell, a stem cell-derived somatic cell, including an induced pluripotent stem cell-derived cell, by contacting with progerin or a progerin-like protein. Methods described by the present disclosure can produce age-appropriate cells from a somatic cell or a stem cell, such as an old cell and/or a mature cell. Such age-modified cells constitute model systems for the study of late-onset diseases and/or disorders.
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公开(公告)号：US20160201032A1
公开(公告)日：2016-07-14
申请号：US15077012
申请日：2016-03-22
发明人： Lorenz Studer , Stuart M. Chambers
摘要： The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC), human induced pluripotent stem cells (hiPSC), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC to nociceptors (i.e. nociceptor cells) using novel culture conditions. The nociceptors made using the methods of the present invention are further contemplated for various uses including, but limited to, use in in vitro drug discovery assays, pain research, and as a therapeutic to reverse disease of, or damage to, the peripheral nervous system (PNS). Further, compositions and methods are provided for producing melanocytes from human pluripotent stem cells for use in disease modeling.
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公开(公告)号：US20240263134A1
公开(公告)日：2024-08-08
申请号：US18591734
申请日：2024-02-29
发明人： Lorenz Studer , Jae-Won Shim , Sonja Kriks
IPC分类号： C12N5/0793 , A61K35/30 , C12N5/0797
CPC分类号： C12N5/0619 , A61K35/30 , C12N5/0623 , C12N2501/01 , C12N2501/119 , C12N2501/13 , C12N2501/15 , C12N2501/16 , C12N2501/41 , C12N2501/415 , C12N2501/999 , C12N2506/02 , C12N2506/45
摘要： The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC) in addition to nonembryonic human induced pluripotent stem cells (hiPSC), somatic stem cells, stem cells from patients with a disease, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC into floor plate midbrain progenitor cells and then further into large populations of midbrain fate FOXA2+LMX1A+TH+ dopamine (DA) neurons using novel culture conditions. The midbrain fate FOXA2+LMX1A+TH+ dopamine (DA) neurons made using the methods of the present invention are further contemplated for various uses including, but not limited to, use in in vitro drug discovery assays, neurology research, and as a therapeutic to reverse disease of, or damage to, a lack of dopamine neurons in a patient. Further, compositions and methods are provided for differentiating midbrain fate FOXA2+LMX1A+TH+ dopamine (DA) neurons from human pluripotent stem cells for use in disease modeling, in particular Parkinson's disease. Additionally, authentic DA neurons are enriched for markers, such as CD142, and A9 type neuronal cells.
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16.发明公开公开(公告)号：US20230365928A1
公开(公告)日：2023-11-16
申请号：US18296779
申请日：2023-04-06
发明人： Lorenz Studer , Asif M. Maroof , Stewart Anderson
IPC分类号： C12N5/0793 , G01N33/50
CPC分类号： C12N5/0619 , G01N33/5058 , G01N33/5073 , G01N33/5029 , G01N33/5088 , C12N2501/415 , C12N2506/02 , C12N2501/15 , C12N2501/41 , C12N2501/999 , C12N2502/08
摘要： Provided are cortical interneurons and other neuronal cells and in vitro methods for producing such cortical interneurons and other neuronal cells by the directed differentiation of stem cells and neuronal progenitor cells. The present disclosure relates to novel methods of in vitro differentiation of stem cells and neural progenitor cells to produce several type neuronal cells and their precursor cells, including cortical interneurons, hypothalamic neurons and pre-optic cholinergic neurons. The present disclosure describes the derivation of these cells via inhibiting SMAD and Wnt signaling pathways and activating SHH signaling pathway. The present disclosure relates to the novel discovery that the timing and duration of SHH activation can be harnessed to direct controlled differentiation of neural progenitor cells into either cortical interneurons, hypothalamic neurons or pre-optic cholinergic neurons. The present disclosure also relates to compositions of cortical interneurons, hypothalamic neurons or pre-optic cholinergic neurons, and their precursors, that are highly enriched and can be used in variety of application. These cells can be used therapeutically to treat neurodegenerative and neuropsychiatric disorders, and can be used for disease modeling and drug screening.
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公开(公告)号：US11649431B2
公开(公告)日：2023-05-16
申请号：US14922110
申请日：2015-10-23
发明人： Lorenz Studer , Asif M. Maroof , Stewart Anderson
IPC分类号： C12N5/0793 , G01N33/50
CPC分类号： C12N5/0619 , G01N33/5029 , G01N33/5058 , G01N33/5073 , G01N33/5088 , C12N2501/15 , C12N2501/41 , C12N2501/415 , C12N2501/999 , C12N2502/08 , C12N2506/02
摘要： Provided are cortical interneurons and other neuronal cells and in vitro methods for producing such cortical interneurons and other neuronal cells by the directed differentiation of stem cells and neuronal progenitor cells. The present disclosure relates to novel methods of in vitro differentiation of stem cells and neural progenitor cells to produce several type neuronal cells and their precursor cells, including cortical interneurons, hypothalamic neurons and pre-optic cholinergic neurons. The present disclose describes the derivation of these cells via inhibiting SMAD and Wnt signaling pathways and activating SHH signaling pathway. The present disclosure relates to the novel discovery that the timing and duration of SHH activation can be harnessed to direct controlled differentiation of neural progenitor cells into either cortical interneurons, hypothalamic neurons or pre-optic cholinergic neurons. The present disclosure also relates to compositions of cortical interneurons, hypothalamic neurons or pre-optic cholinergic neurons, and their precursors, that are highly enriched and can be used in variety of application. These cells can be used therapeutically to treat neurodegenerative and neuropsychiatric disorders, and can be used for disease modeling and drug screening.
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公开(公告)号：US20210214681A1
公开(公告)日：2021-07-15
申请号：US17213830
申请日：2021-03-26
摘要： The present disclosure relates to methods for generating microglial cells derived from stem cells (e.g., human stem cells), microglial cells obtained from such methods and compositions comprising thereof, and uses of said microglial cells for disease modeling and for treating microglia related disorders.
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公开(公告)号：US10858625B2
公开(公告)日：2020-12-08
申请号：US15820941
申请日：2017-11-22
发明人： Lorenz Studer , Stefan Irion , Mark Tomishima , Sonja Kriks
IPC分类号： A61K35/30 , C12N5/0793 , A61K35/545 , A61P25/16
摘要： The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into midbrain dopamine neurons, and precursors thereof, and cells generated by such methods. The presently disclosed subject matter also provides for uses of such cells for treating neurodegenerative disorders.
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公开(公告)号：US20200239841A1
公开(公告)日：2020-07-30
申请号：US16773341
申请日：2020-01-27
发明人： Lorenz Studer , Gustav Cederquist
摘要： The present disclosure relates to methods and compositions for generating topographically organized tissues in vitro, for the resulting cultured tissue and components thereof, and for uses of such cultured tissue and its components in drug discovery, toxicology studies, and therapy.
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