公开(公告)号：US20170168047A1

公开(公告)日：2017-06-15

申请号：US15440191

申请日：2017-02-23

Applicant: Meso Scale Technologies, LLC.

Inventor： Anahit Aghvanyan ,  Eli N. Glezer ,  John Kenten ,  George Sigal ,  Martin Stengelin

IPC: G01N33/543 ,  C12Q1/68 ,  G01N21/66 ,  G01N21/76 ,  G01N21/77 ,  G01N33/53 ,  G01N33/58

CPC classification number: G01N33/54393 ,  C12Q1/6804 ,  G01N21/66 ,  G01N21/76 ,  G01N21/77 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/533 ,  G01N33/54306 ,  G01N33/56988 ,  G01N33/582 ,  G01N2021/7786 ,  C12Q2537/125 ,  C12Q2563/179

Abstract: The present disclosure is directed to a kit for the detection of an analyte of interest in a sample comprising, in one or more vials, containers, or compartments, a. a surface comprising (i) a capture reagent for the analyte, said capture reagent bound to the surface, and (ii) an anchoring reagent bound to an anchoring oligonucleotide sequence, said anchoring reagent bound to the surface, b. a first detection reagent for the analyte that is linked to a first nucleic acid probe, wherein the first nucleic acid probe comprises an extended sequence that is complementary to the anchoring oligonucleotide sequence and c. a second detection reagent for the analyte that is linked to a second nucleic acid probe.

公开(公告)号：US20160245799A1

公开(公告)日：2016-08-25

申请号：US15146293

申请日：2016-05-04

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Kent Johnson ,  Michael Tsionsky ,  John H. Kenten ,  Jeff D. Debad ,  Robert M. Umek ,  Paula Denney Eason ,  Hans Biebuyck ,  Jacob N. Wohlstadter ,  James Wilbur ,  George Sigal ,  Niranjan Y. Sardesai

IPC: G01N33/53 ,  G01N33/573 ,  G01N33/68 ,  G01N33/569

CPC classification number: G01N33/5304 ,  C12Q1/485 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/54386 ,  G01N33/56944 ,  G01N33/56983 ,  G01N33/56988 ,  G01N33/573 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6842 ,  G01N33/6845 ,  G01N2333/11 ,  G01N2333/135 ,  G01N2333/16 ,  G01N2333/525 ,  G01N2333/5412 ,  G01N2333/545 ,  G01N2333/57 ,  G01N2333/71 ,  G01N2333/9121 ,  Y10S436/807

Abstract: Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

Abstract translation: 使用具有多个测定域的测定模块进行多重测试测量。 在优选实施方案中，这些测量在具有集成电极的测定模块中进行，所述测量模块具有适于接收测定模块的读取器装置，在测定模块的孔或测定区域中诱导发光，优选电极诱导的发光并测量诱导的发光。

公开(公告)号：US20140256588A1

公开(公告)日：2014-09-11

申请号：US14203638

申请日：2014-03-11

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Sudeep Kumar ,  Pankaj Oberoi ,  George Sigal ,  Michael Tsionsky

IPC: C12Q1/68

CPC classification number: B01L3/50853 ,  B01L2300/021 ,  B01L2300/043 ,  B01L2300/0609 ,  B01L2300/0829 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/6845 ,  G01N2458/10 ,  C12Q2563/131

Abstract: The invention relates to methods for conducting solid-phase binding assays. One example is an assay method having improved analyte specificity where specificity is limited by the presence of non-specific binding interactions.

Abstract translation: 本发明涉及用于进行固相结合测定的方法。 一个实例是具有改进的分析物特异性的测定方法，其中特异性受到非特异性结合相互作用的存在的限制。

公开(公告)号：US20230279290A1

公开(公告)日：2023-09-07

申请号：US18145735

申请日：2022-12-22

Applicant: Meso Scale Technologies, LLC.

Inventor： John Kenten ,  John Pettersson ,  George Sigal ,  Alexander K. Tucker-Schwartz

IPC: C09K11/02 ,  C12Q1/6816

CPC classification number: C09K11/02 ,  C12Q1/6816

Abstract: The disclosure relates to novel compositions comprising an electrochemiluminescence (ECL) co-reactant. In embodiments, the composition further comprises an ionic component, a surfactant, or combination thereof. In embodiments, the ECL co-reactant is triethanolamine (TEA), tert-butyldiethanolamine (tBDEA), methyldibutylethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), or a combination thereof. Methods of using the compositions and kits comprising the compositions are also provided herein, including methods using ECL-labeled oligonucleotide probes having quenching moieties.

公开(公告)号：US11242570B2

公开(公告)日：2022-02-08

申请号：US15696953

申请日：2017-09-06

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  George Sigal

IPC: C12Q1/70 ,  C12Q1/6813 ,  G01N33/542 ,  G01N33/543 ,  C12Q1/6834

Abstract: The present invention is directed to methods for reducing cross-reactivity between species employed in multiplexed immunoassays.

公开(公告)号：US20220033918A1

公开(公告)日：2022-02-03

申请号：US17504937

申请日：2021-10-19

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  George Sigal

IPC: C12Q1/70 ,  C12Q1/6813 ,  G01N33/542 ,  G01N33/543 ,  C12Q1/6834

Abstract: The present invention is directed to methods for reducing cross-reactivity between species employed in multiplexed immunoassays.

公开(公告)号：US10814327B2

公开(公告)日：2020-10-27

申请号：US15646578

申请日：2017-07-11

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Stephen Higgins ,  Sandor Kovacs ,  Sudeep Kumar ,  Kenneth Page ,  Kristian Roth ,  George Sigal

IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/68 ,  C12Q1/686

Abstract: Assay cartridges are described that have purification, reaction, and detection zones and other fluidic components which can include sample chambers, waste chambers, conduits, vents, reagent chambers, reconstitution chambers and the like. The assay cartridges are used to conduct multiplexed nucleic acid measurements. Also described are kits including such cartridges, methods of using the same, and a reader configured to analyze an assay conducted using an assay cartridge.

公开(公告)号：US20190291103A1

公开(公告)日：2019-09-26

申请号：US16227081

申请日：2018-12-20

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Sudeep Kumar ,  Pankaj Oberoi ,  George Sigal ,  Michael Tsionsky

IPC: B01L3/00 ,  C12Q1/6804 ,  G01N33/68 ,  G01N33/543 ,  C12Q1/6816 ,  C12Q1/6876

Abstract: The invention relates to methods for conducting solid-phase binding assays. One example is an assay method having improved analyte specificity where specificity is limited by the presence of non-specific binding interactions.

公开(公告)号：US10408823B2

公开(公告)日：2019-09-10

申请号：US15311309

申请日：2015-05-15

Applicant: MESO SCALE TECHNOLOGIES, LLC.

Inventor： Anahit Aghvanyan ,  Eli N. Glezer ,  John Kenten ,  George Sigal ,  Martin Stengelin ,  David Routenberg

IPC: C12Q1/6832 ,  C12Q1/6837 ,  G01N33/543 ,  C12Q1/6844

Abstract: The present invention is directed to methods for improving assay specificity and performance in binding assays.

公开(公告)号：US20190257823A1

公开(公告)日：2019-08-22

申请号：US16401513

申请日：2019-05-02

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Kent Johnson ,  Michael Tsionsky ,  John H. Kenten ,  Jeff D. Debad ,  Robert M. Umek ,  Paula Denney Eason ,  Hans Biebuyck ,  Jacob N. Wohlstadter ,  James Wilbur ,  George Sigal ,  Niranjan Y. Sardesai

IPC: G01N33/53 ,  G01N33/68 ,  G01N33/573 ,  G01N33/569 ,  C12Q1/48 ,  G01N33/543

Abstract: Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.