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    Use of recombinant live-attenuated parainfluenza virus (PIV) as a vector to protect against disease caused by PIV and respiratory syncytial virus (RSV)
    15.
    发明授权
    Use of recombinant live-attenuated parainfluenza virus (PIV) as a vector to protect against disease caused by PIV and respiratory syncytial virus (RSV) 有权
    使用重组减毒副流感病毒（PIV）作为载体，以防止由PIV和呼吸道合胞病毒（RSV）引起的疾病

    公开(公告)号：US07314631B1

    公开(公告)日：2008-01-01

    申请号：US09458813

    申请日：1999-12-10

    申请人： Brian R. Murphy ,  Peter L. Collins ,  Anna P. Durbin ,  Mario H. Skiadopoulos ,  Tao Tao

    发明人： Brian R. Murphy ,  Peter L. Collins ,  Anna P. Durbin ,  Mario H. Skiadopoulos ,  Tao Tao

    IPC分类号： A61K39/155 ,  C12N7/00

    CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/155 ,  A61K39/165 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/70 ,  C07K14/005 ,  C12N2760/18422 ,  C12N2760/18434 ,  C12N2760/18634 ,  C12N2760/18643 ,  C12N2760/18734

    摘要： Chimeric parainfluenza viruses (PIVs) are provided that incorporate a PIV vector genome or antigenome and one or more antigenic determinant(s) of a heterologous PIV or non-PIV pathogen. These chimeric viruses are infectious and attenuated in humans and other mammals and are useful in vaccine formulations for eliciting and immune responses against one or more PIVs, or against a PIV and non-PIV pathogen. Also provided are isolated polynucleotide molecules and vectors incorporating a chimeric PIV genome or antigenome which includes a partial or complete PIV vector genome or antigenome combined or integrated with one or more heterologous gene(s) or genome segment(s) encoding antigenic determinant(s) of a heterologous PIV or non-PIV pathogen. In preferred aspects of the invention, chimeric PIV incorporate a partial or complete human PIV vector genome or antigenome combined with one or more heterologous gene(s) or genome segment(s) from a heterologous PIV or non-PIV pathogen, wherein the chimeric virus is attenuated for use as a vaccine agent by any of a variety of mutations and nucleotide modifications introduced into the chimeric genome or antigenome.

    摘要翻译： 提供了嵌合副流感病毒（PIV），其包含PIV载体基因组或反义基因组以及异源PIV或非PIV病原体的一个或多个抗原决定簇。 这些嵌合病毒在人类和其他哺乳动物中是感染性和减毒性的，并且可用于针对一种或多种PIV或针对PIV和非PIV病原体的引发和免疫应答的疫苗制剂。 还提供了分离的多核苷酸分子和掺入嵌合PIV基因组或抗原组的载体，其包括与编码抗原决定簇的一个或多个异源基因或基因组片段组合或整合的部分或完整PIV载体基因组或反义基因， 的异源PIV或非PIV病原体。 在本发明的优选方面，嵌合PIV掺入与来自异源PIV或非PIV病原体的一个或多个异源基因或基因组片段组合的部分或完整的人PIV载体基因组或反向异构体，其中所述嵌合病毒 通过引入到嵌合基因组或反基因组中的各种突变和核苷酸修饰中的任一种减毒用作疫苗剂。

    PRODUCTION OF ATTENUATED NEGATIVE STRANDED RNA VIRUS VACCINES FROM CLONED NUCLEOTIDE SEQUENCES
    16.
    发明申请
    PRODUCTION OF ATTENUATED NEGATIVE STRANDED RNA VIRUS VACCINES FROM CLONED NUCLEOTIDE SEQUENCES 审中-公开
    从克隆核苷酸序列中产生衰减的病毒RNA病毒疫苗

    公开(公告)号：US20120064569A9

    公开(公告)日：2012-03-15

    申请号：US12136765

    申请日：2008-06-10

    申请人： Brian R. Murphy ,  Peter L. Collins ,  Anna P. Durbin ,  Mario H. Skiadopoulos

    发明人： Brian R. Murphy ,  Peter L. Collins ,  Anna P. Durbin ,  Mario H. Skiadopoulos

    IPC分类号： C12P21/00 ,  C12N15/63 ,  C12N7/01 ,  C12N15/44

    CPC分类号： A61K39/155 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/543 ,  A61K2039/544 ,  C12N7/00 ,  C12N2760/18534 ,  C12N2760/18561 ,  C12N2760/18634

    摘要： Attenuated, recombinant negative stranded RNA viruses suitable for vaccine use are produced from one or more isolated polynucleotide molecules encoding the virus. A recombinant genome or antigenome of the subject virus is modified to encode a mutation within a recombinant protein of the virus at one or more amino acid positions(s) corresponding to a site of an attenuating mutation in a heretologous, mutant negative stranded RNA virus. A similar attenuating mutation as identified in the heterologous negative stranded RNA virus is thus incorporated at a corresponding site within the recombinant virus to confer an attenuated phenotype on the recombinant virus. The attenuating mutation incorporated in the recombinant virus may be identical or conservative in relation to the attenuating mutation identified in the heterologous, mutant virus. By the transfer of mutations into recombinant negative stranded RNA viruses in this matter, candidate vaccine viruses are engineered to elicit a desired immune response against a subject virus in a host susceptible to infection thereby.

    摘要翻译： 减毒，从编码病毒的一个或多个分离的多核苷酸分子产生适合疫苗使用的重组负链RNA病毒。 修饰受试病毒的重组基因组或反基因组，以在对应于本体异质突变体负链RNA病毒中的减毒突变位点的一个或多个氨基酸位置上编码病毒重组蛋白内的突变。 因此，在异源负链RNA病毒中鉴定的类似的减毒突变被并入重组病毒中的相应位点，以在重组病毒上赋予减毒表型。 结合在重组病毒中的减毒突变可以与异源突变病毒中鉴定的减毒突变相同或保守。 通过将突变转移到重组负链RNA病毒中，候选疫苗病毒被工程化以在由此感染的宿主中引发针对受试者病毒的所需免疫应答。

    Construction and use of recombinant parainfluenza viruses expressing a chimeric glycoprotein
    19.
    发明授权
    Construction and use of recombinant parainfluenza viruses expressing a chimeric glycoprotein 有权
    构建和使用表达嵌合糖蛋白的重组副流感病毒

    公开(公告)号：US07250171B1

    公开(公告)日：2007-07-31

    申请号：US09459062

    申请日：1999-12-10

    申请人： Tao Tao ,  Mario H. Skiadopoulos ,  Peter L. Collins ,  Brian R. Murphy

    发明人： Tao Tao ,  Mario H. Skiadopoulos ,  Peter L. Collins ,  Brian R. Murphy

    IPC分类号： A61K31/155 ,  C12N15/00

    CPC分类号： C12N15/86 ,  A61K2039/5256 ,  C07K2319/00 ,  C12N2760/18643

    摘要： Chimeric parainfluenza viruses (PIVs) are provided that incorporate a PIV vector genome or antigenome modified to encode a chimeric glycoprotein incorporating one or more heterologous antigenic domains, fragments, or epitopes of a second, antigenically distinct HPIV. These chimeric viruses are infectious and attenuated in humans and other mammals and are useful in vaccine formulations for eliciting an immune responses against one or more PIVs, and, optionally against respiratory syncytial virus (RSV). Also provided are isolated polynucleotide molecules and vectors incorporating a chimeric PIV genome or antigenome which includes a HPIV vector genome or antigenome combined or integrated with one or more heterologous genome segment(s) encoding one or more antigenic determinant(s) of a heterologous PIV to encode a chimeric glycoprotein. In preferred aspects of the invention, the chimeric virus is attenuated for use as a vaccine agent by additional mutations or nucleotide modifications introduced into the chimeric genome or antigenome.

    摘要翻译： 提供了嵌合副流感病毒（PIV），其包含PIV载体基因组或经修饰的反义基因组，以编码掺入一个或多个异源抗原结构域，片段或第二抗原性不同HPIV表位的嵌合糖蛋白。 这些嵌合病毒在人和其他哺乳动物中是感染性和减毒性的，并且可用于引发针对一种或多种PIV的免疫应答的疫苗制剂，以及任选地抗呼吸道合胞病毒（RSV）。 还提供了分离的多核苷酸分子和掺入嵌合PIV基因组或反基因组的载体，其包括与编码异源PIV的一个或多个抗原决定簇的一个或多个异源基因组片段组合或整合的HPIV载体基因组或抗原子集， 编码嵌合糖蛋白。 在本发明的优选方面，嵌合病毒通过引入到嵌合基因组或反基因组中的附加突变或核苷酸修饰来减毒用作疫苗剂。