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    POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS
    12.
    发明申请
    POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS 审中-公开
    使用多不饱和脂多糖合成酶系统进行异构体生物聚合脂肪酸生产

    公开(公告)号：US20070245431A1

    公开(公告)日：2007-10-18

    申请号：US11686850

    申请日：2007-03-15

    申请人： James Metz ,  Jerry Kuner ,  James Lippmeier ,  Maurice Moloney ,  Cory Nykiforuk

    发明人： James Metz ,  Jerry Kuner ,  James Lippmeier ,  Maurice Moloney ,  Cory Nykiforuk

    IPC分类号： A01H1/00 ,  C12N9/04 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

    摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the plant or plant cell, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

    摘要翻译： 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物表达的脂肪酸合成酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加植物或植物细胞中丙二酰辅酶A的水平 ，并且在一个方面，抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

    Modified oleosins
    13.
    发明申请
    Modified oleosins 审中-公开
    改性油质蛋白

    公开(公告)号：US20070006343A1

    公开(公告)日：2007-01-04

    申请号：US10561178

    申请日：2004-06-18

    申请人： Maurice Moloney ,  Rodrigo Siloto

    发明人： Maurice Moloney ,  Rodrigo Siloto

    IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C07K14/415 ,  C07K2319/00 ,  C12N15/8221 ,  C12N15/8257

    摘要： The present invention describes novel polypeptide structures based on oleosin molecules which are capable of being targeted to oil bodies in plants. The modified oleosin polypeptides are obtained by performing modifications in the hydrophobic domain encoding sequence of an oleosin cDNA. The present invention describes methods to obtain such polypeptides in vivo. The novel oleosins may be used to deliver a recombinant (non-oleosin) protein to oil bodies.

    摘要翻译： 本发明描述了能够靶向植物油体的基于油质蛋白分子的新型多肽结构。 通过在油质蛋白cDNA的疏水结构域编码序列中进行修饰来获得修饰的油质蛋白多肽。 本发明描述了在体内获得这种多肽的方法。 新的油质蛋白可用于将重组（非油质蛋白）蛋白递送至油体。

    Oil-body proteins as carriers of high-value peptides in plants
    14.
    发明授权
    Oil-body proteins as carriers of high-value peptides in plants 失效
    油体蛋白作为植物中高价值肽的载体

    公开(公告)号：US5650554A

    公开(公告)日：1997-07-22

    申请号：US366783

    申请日：1994-12-30

    申请人： Maurice Moloney

    发明人： Maurice Moloney

    IPC分类号： C07K14/545 ,  C07K14/61 ,  C07K14/81 ,  C07K14/815 ,  C12N9/64 ,  C12N9/74 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04

    CPC分类号： C12N9/6483 ,  C07K14/545 ,  C07K14/61 ,  C07K14/8139 ,  C07K14/815 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12N9/6429 ,  C12Y304/21005 ,  C12Y304/23004 ,  C07K2319/00

    摘要： The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oleosins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

    摘要翻译： 本发明涉及具有独特特征的称为油体蛋白基因的一类基因的用途。 这些特征的发现允许本发明用于生产重组蛋白质的方法，其中感兴趣的蛋白质可以容易地与其他宿主细胞组分分离。 本发明进一步举例说明了利用油体蛋白和油体基因在许多生物体，特别是植物种子中表达目标多肽的独特特征的方法。 所述多肽可以包括但不限于：种子储存蛋白，酶，生物活性肽，抗体等。 还可以修饰本发明以从非植物宿主细胞中回收与油质蛋白融合的重组多肽。 另外，本发明提供了一种使用与种子发芽期间释放的种子油体相关的重组蛋白的方法，用于表达能够保护来自病原体的幼苗的多肽。 最后，油体蛋白与油体的持续结合可以进一步利用，以开发生物学手段来产生可用于生物转化底物的新型固定化酶。

    Modification of fatty acid metabolism in plants
    15.
    发明授权
    Modification of fatty acid metabolism in plants 失效
    植物脂肪酸代谢的修饰

    公开(公告)号：US06586658B1

    公开(公告)日：2003-07-01

    申请号：US09389395

    申请日：1999-09-03

    申请人： Oliver P. Peoples ,  Maurice Moloney ,  Nii Patterson ,  Kristi D. Snell

    发明人： Oliver P. Peoples ,  Maurice Moloney ,  Nii Patterson ,  Kristi D. Snell

    IPC分类号： A01H100

    CPC分类号： C12N15/8289 ,  C12N15/52 ,  C12N15/8243 ,  C12N15/8247

    摘要： Methods and systems to modify fatty acid biosynthesis and oxidation in plants to make new polymers are provided. Two enzymes are essential: a hydratase such as D-specific enoyl-CoA hydratase, for example, the hydratase obtained from Aeromonas caviae, and a &bgr;-oxidation enzyme system. Some plants have a &bgr;-oxidation enzyme system which is sufficient to modify polymer synthesis when the plants are engineered to express the hydratase. Examples demonstrate production of polymer by expression of these enzymes in transgenic plants. Examples also demonstrate that modifications in fatty acid biosynthesis can be used to alter plant phenotypes, decreasing or eliminating seed production and increasing green plant biomass, as well as producing polyhydroxyalkanoates.

    摘要翻译： 提供了修饰植物脂肪酸生物合成和氧化以制备新聚合物的方法和系统。 两种酶是必需的：水合酶如D-特异性烯酰辅酶A水合酶，例如从气单胞菌（Aeromonas caviae）获得的水合酶和β-氧化酶系统。 一些植物具有β-氧化酶系统，当植物被工程化以表达水合酶时，其足以改变聚合物合成。 实施例证明通过在转基因植物中表达这些酶来生产聚合物。 实例还表明，脂肪酸生物合成中的修饰可用于改变植物表型，减少或消除种子生产和增加绿色植物生物量，以及生产聚羟基链烷酸酯。