公开(公告)号：US08875713B2

公开(公告)日：2014-11-04

申请号：US13239498

申请日：2011-09-22

申请人： James Metz

发明人： James Metz

IPC分类号： A61F5/56

CPC分类号： A61F5/566

摘要： An anti-obstructive airway dental orthotic is described. The orthotic includes a maxillary retainer and a mandibular retainer releasably attaching to a plurality of teeth of a wearer. The retainers are attached by at least one strut, either variable in length or of a plurality of lengths, which exert a forward force on the lower jaw, tending to advance the mandible relative to the maxilla. This advancement of the lower jaw promotes a less obstructed airway in the wearer, while fenestrations in the retainers may allow for greater comfort and tongue movement.

摘要翻译： 描述了一种抗阻塞性气道牙科矫形器。 矫形器包括上颌保持器和可释放地附接到穿戴者的多个牙齿的下颌保持器。 保持器通过至少一个长度或多个长度的支柱连接，其在下颌上施加向前的力，倾向于相对于上颌骨推进下颌骨。 下颌的这种进步促进穿用者较少阻塞的气道，而保持器中的开窗可以允许更大的舒适性和舌头移动。

公开(公告)号：US20080044874A1

公开(公告)日：2008-02-21

申请号：US11781875

申请日：2007-07-23

申请人： Craig Weaver ,  Ross Zirkle ,  James Metz

发明人： Craig Weaver ,  Ross Zirkle ,  James Metz

IPC分类号： C12P7/64 ,  C12N1/13 ,  C12N1/21 ,  C12N15/52 ,  C12N15/74 ,  C12N5/10

CPC分类号： C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

摘要： Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

摘要翻译： 公开了来自细菌微生物Shewanella japonica和Shewanella olleyana的完全多不饱和脂肪酸（PUFA）聚酮化合物（PKS）系统及其生物活性片段和同系物。 更具体地，本发明涉及编码这种PUFA PKS系统的核酸，其包含这种PUFA PKS系统的蛋白质和结构域到包含这种PUFA PKS系统的转基因生物（植物和微生物），以及制备和使用 本文公开的PUFA PKS系统。 本发明还涉及基因修饰的植物和微生物以及通过操作PUFA聚酮化合物合酶（PKS）系统来有效地产生富含多种不饱和脂肪酸（PUFA）以及其它生物活性分子的脂质的方法。

公开(公告)号：US20080044870A1

公开(公告)日：2008-02-21

申请号：US11778605

申请日：2007-07-16

申请人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

发明人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/12 ,  C12N1/21 ,  C12N5/04

CPC分类号： C12N15/52 ,  A23D7/001 ,  A23L33/115 ,  A23L33/135 ,  C07K14/28 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080044869A1

公开(公告)日：2008-02-21

申请号：US11778604

申请日：2007-07-16

申请人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

发明人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/21 ,  C12N5/10

CPC分类号： C12N15/52 ,  A23D7/001 ,  A23L33/115 ,  A23L33/135 ,  C07K14/28 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

摘要翻译： 本发明一般涉及多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物分离到分离的核酸分子和编码这种PUFA PKS系统的生物活性结构域的重组核酸分子，所述基因修饰的生物体包含PUFA PKS 系统，制备和使用这些系统用于生产感兴趣的生物活性分子的方法，以及用于鉴定具有这种PUFA PKS系统的新的细菌和非细菌微生物的新方法。

公开(公告)号：US20080044868A1

公开(公告)日：2008-02-21

申请号：US11778575

申请日：2007-07-16

申请人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

发明人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

IPC分类号： C12P7/64 ,  C12N1/00 ,  C12N1/21

CPC分类号： C12N15/52 ,  A23D7/001 ,  A23L33/115 ,  A23L33/135 ,  C07K14/28 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080038799A1

公开(公告)日：2008-02-14

申请号：US11781870

申请日：2007-07-23

申请人： Craig Weaver ,  Ross Zirkle ,  James Metz

发明人： Craig Weaver ,  Ross Zirkle ,  James Metz

IPC分类号： C07H21/04 ,  C12N5/04 ,  C12P7/64

CPC分类号： C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

摘要： Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

公开(公告)号：US20080038798A1

公开(公告)日：2008-02-14

申请号：US11781867

申请日：2007-07-23

申请人： Craig Weaver ,  Ross Zirkle ,  James Metz

发明人： Craig Weaver ,  Ross Zirkle ,  James Metz

IPC分类号： C12N1/20 ,  C07H21/04 ,  C12N5/10 ,  C12P7/64

CPC分类号： C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

摘要： Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

公开(公告)号：US20080038792A1

公开(公告)日：2008-02-14

申请号：US11777279

申请日：2007-07-12

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/12 ,  C12N1/21 ,  C12N5/04

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080032367A1

公开(公告)日：2008-02-07

申请号：US11781871

申请日：2007-07-23

申请人： Craig Weaver ,  Ross Zirkle ,  James Metz

发明人： Craig Weaver ,  Ross Zirkle ,  James Metz

IPC分类号： C12P7/64 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C12N5/04

CPC分类号： C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

摘要： Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

公开(公告)号：US20070270494A1

公开(公告)日：2007-11-22

申请号：US11686856

申请日：2007-03-15

申请人： James Metz ,  Jerry Kuner ,  James Lippmeier

发明人： James Metz ,  Jerry Kuner ,  James Lippmeier

IPC分类号： A61K31/20 ,  A01H5/00 ,  C07H21/00 ,  C12N1/00 ,  C12N15/82 ,  C12N9/00 ,  C12N5/10 ,  C12N15/74 ,  C11B11/00 ,  A23D7/00

CPC分类号： C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

摘要翻译： 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物体表达的脂肪酸合酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加生物体中丙二酰辅酶A的水平，以及 一方面抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。