公开(公告)号：US5808029A

公开(公告)日：1998-09-15

申请号：US444793

申请日：1995-05-19

申请人： Manfred Brockhaus ,  Zlatko Dembic ,  Reiner Gentz ,  Werner Lesslauer ,  Hansruedi Lotscher ,  Ernst-Jurgen Schlaeger

发明人： Manfred Brockhaus ,  Zlatko Dembic ,  Reiner Gentz ,  Werner Lesslauer ,  Hansruedi Lotscher ,  Ernst-Jurgen Schlaeger

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P29/00 ,  A61P31/04 ,  A61P37/06 ,  C07K1/16 ,  C07K1/22 ,  C07K1/26 ,  C07K4/12 ,  C07K5/10 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/47 ,  C07K14/52 ,  C07K14/525 ,  C07K14/705 ,  C07K14/715 ,  C07K16/06 ,  C07K16/24 ,  C07K16/28 ,  C12N1/21 ,  C12N5/10 ,  C12N15/02 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/53

CPC分类号： C07K14/7151 ,  C07K16/2866 ,  A61K38/00 ,  C12N2799/026

摘要： The present invention is concerned with non-soluble proteins and soluble or insoluble fragments thereof, which bind TNF, in homogeneous form, as well as their physiologically compatible salts, especially those proteins having a molecular weight of about 55 or 75 kD (non-reducing SDS-PAGE conditions), a process for the isolation of such proteins, antibodies against such proteins, DNA sequences which code for non-soluble proteins and soluble or non-soluble fragments thereof, which bind TNF, as well as those which code for proteins comprising partly of a soluble fragment, which binds TNF, and partly of all domains except the first of the constant region of the heavy chain of human immunoglobulins and the recombinant proteins coded thereby as well as a process for their manufacture using transformed pro- and eukaryotic host cells.

摘要翻译： 本发明涉及不溶性蛋白质及其可溶性或不溶性片段，其以均质形式结合TNF，以及它们的生理相容性盐，特别是分子量为约55或75kD的蛋白质（非还原型 SDS-PAGE条件），分离这些蛋白质的方法，针对这些蛋白质的抗体，编码结合TNF的不溶性蛋白质的DNA序列和可溶性或非可溶性片段，以及编码蛋白质的蛋白质 其部分包含结合TNF的可溶性片段，以及部分除了人免疫球蛋白重链的恒定区域的第一个区域以外的所有结构域，以及由此编码的重组蛋白质以及其使用转化的真核和真核生物的方法 宿主细胞。

公开(公告)号：US5610279A

公开(公告)日：1997-03-11

申请号：US95640

申请日：1993-07-21

申请人： Manfred Brockhaus ,  Zlatko Dembic ,  Reiner Gentz ,  Werner Lesslauer ,  Hansruedi Lotscher ,  Ernst-Jurgen Schlaeger

发明人： Manfred Brockhaus ,  Zlatko Dembic ,  Reiner Gentz ,  Werner Lesslauer ,  Hansruedi Lotscher ,  Ernst-Jurgen Schlaeger

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P29/00 ,  A61P31/04 ,  A61P37/06 ,  C07K1/16 ,  C07K1/22 ,  C07K1/26 ,  C07K4/12 ,  C07K5/10 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/47 ,  C07K14/52 ,  C07K14/525 ,  C07K14/705 ,  C07K14/715 ,  C07K16/06 ,  C07K16/24 ,  C07K16/28 ,  C12N1/21 ,  C12N5/10 ,  C12N15/02 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/53 ,  C07K16/46

CPC分类号： C07K14/7151 ,  C07K16/2866 ,  A61K38/00 ,  C12N2799/026

摘要： The present invention is concerned with non-soluble proteins and soluble or insoluble fragments thereof, which bind TNF, in homogeneous form, as well as their physiologically compatible salts, especially those proteins having a molecular weight of about 55 or 75 kD (non-reducing SDS-PAGE conditions), a process for the isolation of such proteins, antibodies against such proteins, DNA sequences which code for non-soluble proteins and soluble or non-soluble fragments thereof, which bind TNF, as well as those which code for proteins comprising partly of a soluble fragment, which binds TNF, and partly of all domains except the first of the constant region of the heavy chain of human immunoglobulins and the recombinant proteins coded thereby as well as a process for their manufacture using transformed pro- and eukaryotic host cells.

摘要翻译： 本发明涉及不溶性蛋白质及其可溶性或不溶性片段，其以均质形式结合TNF，以及它们的生理相容性盐，特别是分子量为约55或75kD的蛋白质（非还原型 SDS-PAGE条件），分离这些蛋白质的方法，针对这些蛋白质的抗体，编码结合TNF的不溶性蛋白质的DNA序列和可溶性或非可溶性片段，以及编码蛋白质的蛋白质 其部分包含结合TNF的可溶性片段，以及部分除了人免疫球蛋白重链的恒定区域的第一个区域以外的所有结构域，以及由此编码的重组蛋白质以及其使用转化的真核和真核生物的方法 宿主细胞。