公开(公告)号：US20070248957A1

公开(公告)日：2007-10-25

申请号：US11011452

申请日：2004-12-14

申请人： Michael Nova ,  Andrew Senyei ,  Zahra Parandoosh ,  Gary David ,  Xiao-Yi Xiao

发明人： Michael Nova ,  Andrew Senyei ,  Zahra Parandoosh ,  Gary David ,  Xiao-Yi Xiao

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G06F19/00 ,  C12M3/00

CPC分类号： C40B30/04 ,  B01J19/0046 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00333 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/0056 ,  B01J2219/00563 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2300/0609 ,  B82Y10/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  C40B60/08 ,  C40B60/14 ,  C40B70/00 ,  G01N35/00732 ,  G01N35/00871 ,  G01N2015/149 ,  G01N2035/00782 ,  G11C13/0014 ,  G11C13/0019 ,  G11C13/025

摘要： Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The data storage units are non-volatile antifuse memories or volatile memories, such as EEPROMS, DRAMS or flash memory. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for electronically tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.

摘要翻译： 提供了具有包含至少一个数据存储单元的具有可远程寻址或远程可编程记录设备的矩阵材料的具有存储器的组合。 基质材料是在固相化学和生物化学合成，免疫测定和杂交反应中用作载体的基质材料。 基质材料还可以包括荧光体或其它发光部分以产生具有记忆的发光基质。 数据存储单元是非易失性反熔丝存储器或易失性存储器，例如EEPROMS，DRAMS或闪存。 通过这种组合，可以通过用识别信息对存储器进行编程来标记与基质组合接近或物理接触的分子和生物颗粒，例如噬菌体和病毒颗粒和细胞，并且可以通过检索存储的 信息。 还提供了基质材料，记忆和连接分子和生物材料的组合。 这些组合具有多种应用，包括组合化学，目标大分子的分离和纯化，用于分析目的的大分子的捕获和检测，污染物的选择性去除，酶催化，细胞分选，药物递送，化学修饰和其它用途。 还提供了用于电子标记分子，生物颗粒和基质支持材料的方法，免疫测定，受体结合测定，闪烁亲近测定，非放射性邻近测定和其它方法。

公开(公告)号：US20090226891A2

公开(公告)日：2009-09-10

申请号：US11011452

申请日：2004-12-14

申请人： Michael Nova ,  Andrew Senyei ,  Zahra Parandoosh ,  Gary David ,  Xiao-Yi Xiao

发明人： Michael Nova ,  Andrew Senyei ,  Zahra Parandoosh ,  Gary David ,  Xiao-Yi Xiao

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G06F19/00 ,  C12M3/00

CPC分类号： C40B30/04 ,  B01J19/0046 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00333 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/0056 ,  B01J2219/00563 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2300/0609 ,  B82Y10/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  C40B60/08 ,  C40B60/14 ,  C40B70/00 ,  G01N35/00732 ,  G01N35/00871 ,  G01N2015/149 ,  G01N2035/00782 ,  G11C13/0014 ,  G11C13/0019 ,  G11C13/025

摘要： Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The data storage units are non-volatile antifuse memories or volatile memories, such as EEPROMS, DRAMS or flash memory. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for electronically tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.

摘要翻译： 提供了具有包含至少一个数据存储单元的具有可远程寻址或远程可编程记录设备的矩阵材料的具有存储器的组合。 基质材料是在固相化学和生物化学合成，免疫测定和杂交反应中用作载体的基质材料。 基质材料还可以包括荧光体或其它发光部分以产生具有记忆的发光基质。 数据存储单元是非易失性反熔丝存储器或易失性存储器，例如EEPROMS，DRAMS或闪存。 通过这种组合，可以通过用识别信息对存储器进行编程来标记与基质组合接近或物理接触的分子和生物颗粒，例如噬菌体和病毒颗粒和细胞，并且可以通过检索存储的 信息。 还提供了基质材料，记忆和连接分子和生物材料的组合。 这些组合具有多种应用，包括组合化学，目标大分子的分离和纯化，用于分析目的的大分子的捕获和检测，污染物的选择性去除，酶催化，细胞分选，药物递送，化学修饰和其它用途。 还提供了用于电子标记分子，生物颗粒和基质支持材料的方法，免疫测定，受体结合测定，闪烁亲近测定，非放射性邻近测定和其它方法。

公开(公告)号：US5972639A

公开(公告)日：1999-10-26

申请号：US901229

申请日：1997-07-24

申请人： Zahra Parandoosh

发明人： Zahra Parandoosh

IPC分类号： C12Q1/42 ,  C12Q1/02

CPC分类号： C12Q1/42 ,  C12Q2334/00

摘要： A method is for determining the number of cells in cell culture is provided. In practicing the method, the cells are lysed and incubated at about 18.degree. C. to 25.degree. C. at acid pH with a benzothiazole substrate for a sufficient time to generate detectable fluorescence that is proportional to cell number, and then measuring fluorescence. The method is highly sensitive and can detect small differences or changes in cell number. Thus, the method can be used in drug screening assays. An automated high throughput screening assay for identifying compounds that modulate angiogenesis using the method is also provided.

摘要翻译： 一种用于确定提供细胞培养物中的细胞数量的方法。 在实施该方法时，将细胞裂解并在酸性pH下用苯并噻唑底物在约18℃至25℃温育足够的时间以产生与细胞数成正比的可检测的荧光，然后测量荧光。 该方法高度敏感，可以检测细胞数量的小差异或变化。 因此，该方法可用于药物筛选测定。 还提供了用于鉴定使用该方法调节血管发生的化合物的自动化高通量筛选测定法。