公开(公告)号：US20180071738A1

公开(公告)日：2018-03-15

申请号：US15559381

申请日：2016-03-18

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Paul Blainey ,  Anthony Kulesa ,  Jared Kehe

IPC分类号： B01L3/00 ,  B01L9/00 ,  C12N15/10 ,  G01N33/68 ,  C12Q1/18 ,  G01N33/533

CPC分类号： B01L3/502784 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00545 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/0065 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/52 ,  B01L9/50 ,  B01L2200/025 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2300/021 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/046 ,  B01L2400/0469 ,  C12N15/1065 ,  C12Q1/18 ,  C40B50/10 ,  C40B60/08 ,  G01N33/533 ,  G01N33/6845 ,  G01N2333/195

摘要： Embodiments disclosed herein are directed to microfluidic devices that allow for scalable on-chip screening of combinatorial libraries and methods of use thereof. Droplets comprising individual molecular species to be screened are loaded onto the microfluidic device. The droplets are labeled by methods known in the art, including but not limited to barcoding, such that the molecular species in each droplet can be uniquely identified. The device randomly sorts the droplets into individual microwells of an array of microwells designed to hold a certain number of individual droplets in order to derive combinations of the various molecular species. The paired droplets are then merged in parallel to form merged droplets in each microwell, thereby avoiding issues associated with single stream merging. Each microwell is then scanned, e.g., using microscopy, such as high content imaging microscopy, to detect the optical labels, thereby identifying the combination of molecular species in each microwell.

公开(公告)号：US08927062B2

公开(公告)日：2015-01-06

申请号：US13740864

申请日：2013-01-14

申请人： Dow Corning Corporation

发明人： Dongchan Ahn ,  Kevin Wier

IPC分类号： C09D5/00 ,  B01J19/00 ,  C40B40/14 ,  C40B50/18 ,  C40B60/08

CPC分类号： C09D5/00 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/0061 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00736 ,  B01J2219/00756 ,  C40B40/14 ,  C40B50/18 ,  C40B60/08 ,  Y10T428/24802 ,  Y10T428/24917 ,  Y10T428/24926

摘要： A method of preparing a substrate with a composition comprising (i) an organoborane initiator and (ii) a radical curable component disposed thereon includes the step of depositing the composition onto the substrate wherein at least one of (i) the organoborane initiator and (ii) the radical curable component is deposited onto the substrate in the form of a gradient pattern. An article comprises the substrate and the gradient pattern formed on the substrate. The gradient pattern is formed from a developed composition comprising the reaction product of (i) the organoborane initiator and (ii) the radical curable component. By forming the gradient pattern on the substrate, combinatorial and high-throughput methods of generating and testing the developed composition are possible, which enable characterization of the developed composition for various physical and chemical properties.

摘要翻译： 一种制备具有组合物的方法，该组合物包含（i）有机硼烷引发剂和（ii）在其上设置的可自由基固化组分，包括将组合物沉积到基材上的步骤，其中（i）有机基硼烷引发剂和（ii ）可自由基固化组分以梯度图案的形式沉积到基底上。 一种制品包括衬底和形成在衬底上的梯度图案。 梯度图案由显影组合物形成，该组合物包含（i）有机硼烷引发剂和（ii）可自由基固化组分的反应产物。 通过在衬底上形成梯度图案，组合和高通量的生成和测试显影组合物的方法是可能的，这使得能够表征用于各种物理和化学性质的显影组合物。

公开(公告)号：US20140256595A1

公开(公告)日：2014-09-11

申请号：US14226482

申请日：2014-03-26

申请人： RAINDANCE TECHNOLOGIES, INC.

发明人： Darren R. Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC分类号： C12Q1/68

CPC分类号： C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US08680024B2

公开(公告)日：2014-03-25

申请号：US13429929

申请日：2012-03-26

申请人： Sean R. Coyer ,  Emmanuel Delamarche ,  Daniel J. Solis

发明人： Sean R. Coyer ,  Emmanuel Delamarche ,  Daniel J. Solis

IPC分类号： C40B50/18

CPC分类号： G01N33/543 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00725 ,  B01J2219/0074 ,  C40B60/08 ,  C40B60/12 ,  G01N33/6845 ,  G01N2333/005

摘要： Methods for screening and arranging microorganisms such as viruses in an array using subtractive contact printing are provided. In one embodiment, a method for forming an array of receptors for microorganisms comprises: patterning an array of structures on a first substrate to form a template on a surface of the first substrate; applying a receptor material to a face of a second substrate; and contacting the face of the second substrate with the template to remove a portion of the receptor material from the second substrate, thereby forming an array of receptors on the second substrate.

公开(公告)号：US07767627B1

公开(公告)日：2010-08-03

申请号：US08847967

申请日：1997-04-22

申请人： Isy Goldwasser ,  Debra A. Ross ,  Peter G. Schultz ,  Xiao-Dong Xiang ,  Gabriel Briceño ,  Xian-Dong Sun ,  Kai-An Wang

发明人： Isy Goldwasser ,  Debra A. Ross ,  Peter G. Schultz ,  Xiao-Dong Xiang ,  Gabriel Briceño ,  Xian-Dong Sun ,  Kai-An Wang

IPC分类号： C40B40/18 ,  C40B50/04 ,  C40B20/02

CPC分类号： B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/0043 ,  B01J2219/00432 ,  B01J2219/00436 ,  B01J2219/00441 ,  B01J2219/00443 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00515 ,  B01J2219/0052 ,  B01J2219/00527 ,  B01J2219/00531 ,  B01J2219/00533 ,  B01J2219/00536 ,  B01J2219/0054 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00646 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00711 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00736 ,  B01J2219/00745 ,  B01J2219/00747 ,  B01J2219/00752 ,  B01J2219/00754 ,  B82Y25/00 ,  B82Y30/00 ,  C01G3/006 ,  C01G21/006 ,  C01G29/006 ,  C01G51/006 ,  C01G51/68 ,  C01P2002/34 ,  C01P2002/52 ,  C01P2004/84 ,  C01P2006/10 ,  C01P2006/32 ,  C01P2006/40 ,  C01P2006/42 ,  C01P2006/60 ,  C04B35/01 ,  C04B35/16 ,  C04B35/4508 ,  C04B35/4521 ,  C04B35/4525 ,  C04B35/62218 ,  C04B2235/3206 ,  C04B2235/3208 ,  C04B2235/3213 ,  C04B2235/3215 ,  C04B2235/3224 ,  C04B2235/3225 ,  C04B2235/3227 ,  C04B2235/3229 ,  C04B2235/3262 ,  C04B2235/327 ,  C04B2235/3275 ,  C04B2235/3284 ,  C04B2235/3286 ,  C04B2235/3296 ,  C04B2235/3298 ,  C04B2235/3418 ,  C04B2235/3463 ,  C04B2235/656 ,  C04B2235/761 ,  C04B2235/762 ,  C04B2235/768 ,  C04B2235/96 ,  C23C14/042 ,  C23C14/087 ,  C23C16/042 ,  C40B20/02 ,  C40B30/08 ,  C40B40/14 ,  C40B40/18 ,  C40B60/08 ,  C40B60/14 ,  C40B70/00 ,  G01R33/16 ,  H01F41/18 ,  H01F41/34 ,  H01L27/18 ,  Y10S436/809 ,  Y10T436/25

摘要： Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials or, alternatively, allowing the components to interact to form at least two different materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, nonbiological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

摘要翻译： 用于制备和使用在其上的预定区域中具有不同材料阵列的基材的方法和装置。 通常通过将材料的组分递送到衬底上的预定区域，并且同时使组分反应以形成至少两种材料，或者允许组分相互作用以形成至少两种不同的材料 材料 可以使用本发明的方法和装置制备的材料包括例如共价网络固体，离子固体和分子固体。 更具体地，可以使用本发明的方法和装置制备的材料包括例如无机材料，金属间材料，金属合金，陶瓷材料，有机材料，有机金属材料，非生物有机聚合物，复合材料（例如无机物 复合材料，有机复合材料或其组合）等。一旦制备，可以对这些材料进行筛选以获得有用的性质，包括例如电，热，机械，形态，光学，磁性，化学或其它性质。 因此，本发明提供了用于并行合成和分析具有有用特性的新型材料的方法。

公开(公告)号：US20090232966A1

公开(公告)日：2009-09-17

申请号：US12405218

申请日：2009-03-16

申请人： Nikhil D. Kalyankar ,  Zachary Fresco ,  Chi-I Lang

发明人： Nikhil D. Kalyankar ,  Zachary Fresco ,  Chi-I Lang

IPC分类号： B05D1/28 ,  B05C1/00

CPC分类号： C40B60/08 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00364 ,  B01J2219/00376 ,  B01J2219/00382 ,  B01J2219/00427 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00659 ,  B01J2219/00702 ,  C40B50/18

摘要： This disclosure provides methods, devices and systems for using a stamp to enhance selectivity between surface layers of a substrate, and to facilitate functionalizing selected layers. An array of flat stamps may be used to concurrently stamp multiple regions of a substrate to transfer one or more substances to the topmost layer or layers of the substrate. If desired, the affected regions of the substrate may be isolated from each other through the use of a reactor plate that, when clamped to the substrate's surface, forms reaction wells in the area of stamping. The stamp area can, if desired, be configured for stamping the substrate after the reactor plate has been fitted, with the individual stamps sized and arranged in a manner that permits stamping within each reaction well. If applied in a combinatorial process, a robotic process may be used to transfer multiple combinations of contact substances and processing chemicals to each reaction well to perform many concurrent processes upon a single substrate (e.g., a single coupon). The methods, devices and systems provided facilitate semiconductor design, optimization and qualification, and may be adapted to production manufacturing.

摘要翻译： 本公开提供了使用印模来增强基底的表面层之间的选择性并促进官能化所选择的层的方法，装置和系统。 可以使用平面阵列阵列来同时印刷衬底的多个区域以将一种或多种物质转移到衬底的最上层或多层。 如果需要，可以通过使用反应器板将基材的受影响区域彼此隔离，当将其夹在基材表面上时，在冲压区域中形成反应孔。 如果需要，邮票区域可以被配置用于在安装反应器板之后冲压衬底，各个邮票的大小和布置方式允许在每个反应井内冲压。 如果应用于组合过程中，机器人过程可用于将接触物质和加工化学品的多种组合转移到每个反应井，以在单个底物（例如，单一试样）上进行许多并行处理。 提供的方法，设备和系统有助于半导体设计，优化和鉴定，并可适用于生产制造。

公开(公告)号：US20090137418A1

公开(公告)日：2009-05-28

申请号：US12265047

申请日：2008-11-05

申请人： Benjamin L. Miller ,  Todd D. Krauss ,  Lewis J. Rothberg ,  Hsin-I Peng

发明人： Benjamin L. Miller ,  Todd D. Krauss ,  Lewis J. Rothberg ,  Hsin-I Peng

IPC分类号： C40B30/04 ,  C40B40/08 ,  C40B50/18 ,  C40B60/12

CPC分类号： C40B50/18 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6825 ,  C40B60/08 ,  G01N21/05 ,  G01N21/64 ,  G01N21/6428 ,  C12Q2525/301

摘要： A sensor chip and detection device are disclosed. The sensor chip includes a substrate, at least a portion of which is covered by a metal nanoparticle film; a first nucleic acid molecule that is characterized by being able to (i) self-anneal into a hairpin conformation and (ii) hybridize specifically to a target nucleic acid molecule, the first nucleic acid molecule having first and second ends, which first end is tethered to the metal nanoparticle film; and a first fluorophore bound to the second end of the first nucleic molecule. When the first nucleic acid molecule is in the hairpin conformation, the metal nanoparticle film substantially quenches fluorescent emissions by the first fluorophore, and when the first nucleic acid molecule is in a non-hairpin conformation fluorescent emissions by the first fluorophore are surface plasmon-enhanced.

摘要翻译： 公开了一种传感器芯片和检测装置。 传感器芯片包括至少一部分被金属纳米颗粒膜覆盖的基板; 第一核酸分子，其特征在于能够（i）自发退火成发夹构象和（ii）与靶核酸分子特异性杂交，所述第一核酸分子具有第一和第二末端，所述第一末端是 拴在金属纳米颗粒膜上; 和与第一核酸分子的第二末端结合的第一荧光团。 当第一核酸分子处于发夹构象时，金属纳米颗粒膜基本上淬灭第一荧光团的荧光发射，并且当第一核酸分子处于非发夹构象时，第一荧光团的荧光发射是表面等离子体增强 。

公开(公告)号：US07354777B2

公开(公告)日：2008-04-08

申请号：US11184702

申请日：2005-07-19

申请人： Mark Whitney Hart ,  Ho-Cheol Kim ,  Robert Dennis Miller ,  Gregory Michael Wallraff

发明人： Mark Whitney Hart ,  Ho-Cheol Kim ,  Robert Dennis Miller ,  Gregory Michael Wallraff

IPC分类号： H01L21/00

CPC分类号： B01L3/5085 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01L2200/12 ,  B01L2300/0609 ,  B01L2300/069 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/165 ,  B01L2300/168 ,  C40B60/08

摘要： A biomolecular array includes a substrate across which is distributed an array of discrete regions of a porous substance formed from a porogen-containing organosilicate material. The porous substance is designed to bind chemical targets useful in biotechnology applications, such as gene expression, protein, antibody, and antigen experiments. The regions are preferably optically isolated from each other and may be shaped to enhance detection of optical radiation emanating from the porous substance, e.g., as a result of irradiation of the regions with ultraviolet light. The discrete regions may be configured as microscopic wells within the substrate, or they may reside on top of the substrate in the form of microscopic mesas.

摘要翻译： 生物分子阵列包括基底，其上分布有由含致孔剂的有机硅酸盐材料形成的多孔物质的离散区域的阵列。 多孔物质被设计用于结合可用于生物技术应用的化学靶，例如基因表达，蛋白质，抗体和抗原实验。 这些区域优选地彼此光学隔离，并且可以被成形为增强从多孔物质发出的光辐射的检测，例如，作为由紫外线照射该区域的结果。 离散区域可以被配置为衬底内的微观阱，或者它们可以以微观台面的形式驻留在衬底的顶部上。

公开(公告)号：US20060256332A1

公开(公告)日：2006-11-16

申请号：US11492736

申请日：2006-07-25

申请人： Perry Sandstrom

发明人： Perry Sandstrom

IPC分类号： G01J3/30

CPC分类号： G01N21/6452 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00439 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00693 ,  B01J2219/00704 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y30/00 ,  C40B60/08 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0229 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6456

摘要： The present invention relates to novel systems, devices, and methods comprising spatial light modulators for use in the reading and synthesis of microarrays. For example, the present invention provides micromirror systems for synthesizing and acquiring data from nucleic acid microarrays and systems for collecting, processing, and analyzing data obtained from a microarray.

公开(公告)号：US06893816B1

公开(公告)日：2005-05-17

申请号：US09063356

申请日：1998-04-21

申请人： Kenneth L. Beattie

发明人： Kenneth L. Beattie

IPC分类号： B01J19/00 ,  B01L3/00 ,  C12Q1/68 ,  C40B60/14 ,  G01N33/552

CPC分类号： B01L3/50255 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00423 ,  B01J2219/00527 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00691 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L2300/0819 ,  B01L2400/0487 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B60/08 ,  C40B60/14 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  C12Q2565/631

摘要： An improved microfabricated apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and terminate on a second surface thereof such that when a test sample is applied to the substrate, it is capable of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.

摘要翻译： 描述了用于进行多个单独和同时结合反应的改进的微制造装置。 该装置包括其上位于离散和分离的结合反应位点的基底。 该装置的特征在于离散和隔离的区域，其延伸穿过所述基底并终止在其第二表面上，使得当将测试样品施加到基底时，其能够在所述结合反应过程中穿透每个这样的区域。 该装置对于通过DNA分子的杂交进行测序特别有用。