公开(公告)号：US20230212596A1

公开(公告)日：2023-07-06

申请号：US18146434

申请日：2022-12-26

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8216

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US11039586B2

公开(公告)日：2021-06-22

申请号：US14209731

申请日：2014-03-13

Applicant: Monsanto Technology, LLC

Inventor： Paul S. Chomet ,  Jonathan C. Lamb ,  Richard J. Lawrence

IPC: A01H1/02

Abstract: The current invention provides methods for creating a block of genetically linked transgenic traits or a megalocus that can be transmitted as a single genetic unit. The present invention further provides methods for trait introgression to other plants, varieties or species using the megaloci of the invention. Also provided are plants, seeds, and plant parts comprising the megaloci.

公开(公告)号：US10793867B2

公开(公告)日：2020-10-06

申请号：US14209828

申请日：2014-03-13

Applicant: Monsanto Technology, LLC

Inventor： Jonathan C. Lamb ,  Richard J. Lawrence

IPC: C12N15/82 ,  C12Q1/6895 ,  C12N9/22

Abstract: The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.

公开(公告)号：US20190169596A1

公开(公告)日：2019-06-06

申请号：US16275200

申请日：2019-02-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Charles L. ARMSTRONG ,  Edward J. Cargill ,  Fenggao Dong ,  Jonathan C. Lamb ,  Huachun W. Larue ,  Richard J. Lawrence ,  Thomas S. Ream

IPC: C12N15/10 ,  C12N9/22 ,  C12N9/12

Abstract: Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

公开(公告)号：US20170166912A1

公开(公告)日：2017-06-15

申请号：US15120110

申请日：2015-02-27

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Naggy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8216

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US20150167010A1

公开(公告)日：2015-06-18

申请号：US14209828

申请日：2014-03-13

Applicant: Monsanto Technology, LLC

Inventor： Jonathan C. Lamb ,  Richard J. Lawrence

IPC: C12N15/82 ,  C12Q1/68

CPC classification number: C12N15/8213 ,  C12N9/22 ,  C12Q1/6895

Abstract: The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.

Abstract translation: 本发明涉及生物技术，并提供用于序列特异性或序列定向的转录激活子样效应物重组酶介导的目的DNA序列整合到宿主基因组中的新方法。 本发明还提供了用于新型植物转化载体和表达盒的方法，其包括嵌合重组酶与植物表达和转化元件的新型组合。 还提供了基因靶向，DNA序列去除，基因组修饰和分子育种的方法。