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公开(公告)号:US20230255157A1
公开(公告)日:2023-08-17
申请号:US17935438
申请日:2022-09-26
Applicant: Monsanto Technology LLC
Inventor: Christopher T. Bauer , David V. Butruille , Edward J. Cargill , Daniel Challis , Fenggao Dong , Fengxing Du , Brian W. Gardunia , Yang Ju Im , Jonathan C. Lamb , Piyaraj Nan Newton , Pierre Sehabiague , Ruth A. Wagner , Shengqiang Zhong
Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.
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公开(公告)号:US10544471B2
公开(公告)日:2020-01-28
申请号:US15707615
申请日:2017-09-18
Applicant: Monsanto Technology LLC
Inventor: Stanton B. Dotson , Fenggao Dong , Frederic Achard , Sam Eathington , Nengbing Tao , Zoe McKiness
IPC: A01H1/04 , C12Q1/6827 , C12Q1/6874 , C12Q1/6895 , G16B30/00 , G16B35/00 , G16C20/60
Abstract: The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.
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公开(公告)号:US12108719B2
公开(公告)日:2024-10-08
申请号:US17218954
申请日:2021-03-31
Applicant: Monsanto Technology LLC
Inventor: Fenggao Dong , Michael Graham , Huachun Larue , Daniel Ovadya
CPC classification number: A01H3/04 , A01H1/06 , A01H5/10 , A01H6/4684 , A01N43/84 , A01H1/08 , A01H6/4678
Abstract: Provided are methods for increasing the efficiency of creating doubled haploid plants by increasing the number of chances of forming a double haploid seed through treatment of a monocot plant with a plant growth regulator. In certain embodiments, maize plants are produced that comprise multiple co-dominant ears. Also provided are plants comprising the potential to generate increased numbers of doubled haploid offspring.
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公开(公告)号:US20210219507A1
公开(公告)日:2021-07-22
申请号:US17218954
申请日:2021-03-31
Applicant: Monsanto Technology LLC
Inventor: Fenggao Dong , Michael Graham , Huachun Larue , Daniel Ovadya
Abstract: Provided are methods for increasing the efficiency of creating doubled haploid plants by increasing the number of chances of forming a double haploid seed through treatment of a monocot plant with a plant growth regulator. In certain embodiments, maize plants are produced that comprise multiple co-dominant ears. Also provided are plants comprising the potential to generate increased numbers of doubled haploid offspring.
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公开(公告)号:US10588280B2
公开(公告)日:2020-03-17
申请号:US15308249
申请日:2015-05-01
Applicant: Monsanto Technology LLC
Inventor: Huachun Larue , Fenggao Dong
IPC: A01H1/08
Abstract: Provided herein are delivery methods of a plant treatment agent to a tissue of a plant, wherein the tissue is contacted with a plant treatment agent and delivery is aided by a centrifugal force applied to the plant. Disclosed herein is the discovery that drastic improvements in treatment delivery occur when plants are centrifuged after being contacted, submerged, unsubmerged, soaked, etc. in a solution containing a plant treatment agent. In one embodiment, haploid plants contacted with a colchicine solution are centrifuged to reveal improved chromosomal doubling rates as compared to control plants.
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公开(公告)号:US20220348943A1
公开(公告)日:2022-11-03
申请号:US17748857
申请日:2022-05-19
Applicant: Monsanto Technology LLC
Inventor: Charles L. Armstrong , Edward J. Cargill , Fenggao Dong , Jonathan C. Lamb , Huachun W. Larue , Richard J. Lawrence , Thomas S. Ream
Abstract: Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.
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公开(公告)号:US11483991B2
公开(公告)日:2022-11-01
申请号:US17049957
申请日:2019-04-25
Applicant: Monsanto Technology LLC
Inventor: Christopher T. Bauer , David V. Butruille , Edward J. Cargill , Daniel Challis , Fenggao Dong , Fengxing Du , Brian W. Gardunia , Yang Ju Im , Jonathan C. Lamb , Piyaraj Nan Newton , Pierre Sehabiague , Ruth A. Wagner , Shengqiang Zhong
Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.
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公开(公告)号:US10544448B2
公开(公告)日:2020-01-28
申请号:US14856733
申请日:2015-09-17
Applicant: Monsanto Technology LLC
Inventor: Stanton B Dotson , Fenggao Dong , Frederic Achard , Sam Eathington , Nengbing Tao , Zoe McKiness
IPC: A01H1/04 , C12Q1/68 , C12Q1/6827 , G16B30/00 , G16B35/00 , G16C20/60 , C12Q1/6895 , C12Q1/6874
Abstract: The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.
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公开(公告)号:US20190169596A1
公开(公告)日:2019-06-06
申请号:US16275200
申请日:2019-02-13
Applicant: MONSANTO TECHNOLOGY LLC
Inventor: Charles L. ARMSTRONG , Edward J. Cargill , Fenggao Dong , Jonathan C. Lamb , Huachun W. Larue , Richard J. Lawrence , Thomas S. Ream
Abstract: Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.
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公开(公告)号:US20190014730A1
公开(公告)日:2019-01-17
申请号:US15745236
申请日:2016-07-15
Applicant: Monsanto Technology LLC
Inventor: Fenggao Dong , Michael Graham , Huachun Larue , Daniel Ovadya
Abstract: Provided are methods for increasing the efficiency of creating doubled haploid plants by increasing the number of chances of forming a double haploid seed through treatment of a monocot plant with a plant growth regulator. In certain embodiments, maize plants are produced that comprise multiple co-dominant ears. Also provided are plants comprising the potential to generate increased numbers of doubled haploid offspring.
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