公开(公告)号：US12241074B2

公开(公告)日：2025-03-04

申请号：US16472140

申请日：2017-12-21

Applicant: Monsanto Technology LLC

Inventor： Ty J. Barten ,  Edward J. Cargill ,  Jonathan C. Lamb ,  Bryce Lemke ,  Linda A. Rymarquis ,  Dennis H. Yang

IPC: C12N15/82 ,  A01H1/06 ,  A01H5/10 ,  A01H6/46 ,  C07K14/415

Abstract: Disclosed herein are plants exhibiting a semi-dwarf phenotype with reduced plant height compared to control wildtype plants. Some of the disclosed semi-dwarf plants comprise at least one non-natural brachytic mutation in which the activity of a BR2 gene is reduced. Also disclosed are methods for producing a semi-dwarf corn plant using a CRISPR based genome editing system.

公开(公告)号：US20180273962A1

公开(公告)日：2018-09-27

申请号：US15765049

申请日：2016-09-30

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Randall Kerstetter ,  Jonathan C. Lamb ,  Thomas S. Ream ,  Shiaw-Pyng Yang ,  Xuefeng Zhou

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C07K14/415 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158

Abstract: The present disclosure provides maize B chromosome genomic loci and methods of using for agronomic practices.

公开(公告)号：US20220348943A1

公开(公告)日：2022-11-03

申请号：US17748857

申请日：2022-05-19

Applicant: Monsanto Technology LLC

Inventor： Charles L. Armstrong ,  Edward J. Cargill ,  Fenggao Dong ,  Jonathan C. Lamb ,  Huachun W. Larue ,  Richard J. Lawrence ,  Thomas S. Ream

IPC: C12N15/82 ,  A01H1/08 ,  C12N9/12 ,  C12N9/22 ,  C12N15/10

Abstract: Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

公开(公告)号：US11483991B2

公开(公告)日：2022-11-01

申请号：US17049957

申请日：2019-04-25

Applicant: Monsanto Technology LLC

Inventor： Christopher T. Bauer ,  David V. Butruille ,  Edward J. Cargill ,  Daniel Challis ,  Fenggao Dong ,  Fengxing Du ,  Brian W. Gardunia ,  Yang Ju Im ,  Jonathan C. Lamb ,  Piyaraj Nan Newton ,  Pierre Sehabiague ,  Ruth A. Wagner ,  Shengqiang Zhong

IPC: A01H1/08 ,  A01H6/46 ,  A01H1/04

Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

公开(公告)号：US20180023062A1

公开(公告)日：2018-01-25

申请号：US15633591

申请日：2017-06-26

Applicant: Monsanto Technology LLC

Inventor： Jonathan C. Lamb ,  Artem G. Evdokimov

IPC: C12N9/12 ,  C07K14/47 ,  C12N15/90 ,  C12N15/82

CPC classification number: C12N9/1241 ,  C07K14/4705 ,  C07K2319/00 ,  C12N15/8213 ,  C12N15/902

Abstract: The invention relates to biotechnology and provides novel compositions and methods for sequence-specific or sequence-directed transcription activator-like effector recombinases. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, and molecular breeding are also provided.

公开(公告)号：US11039586B2

公开(公告)日：2021-06-22

申请号：US14209731

申请日：2014-03-13

Applicant: Monsanto Technology, LLC

Inventor： Paul S. Chomet ,  Jonathan C. Lamb ,  Richard J. Lawrence

IPC: A01H1/02

Abstract: The current invention provides methods for creating a block of genetically linked transgenic traits or a megalocus that can be transmitted as a single genetic unit. The present invention further provides methods for trait introgression to other plants, varieties or species using the megaloci of the invention. Also provided are plants, seeds, and plant parts comprising the megaloci.

公开(公告)号：US10793867B2

公开(公告)日：2020-10-06

申请号：US14209828

申请日：2014-03-13

Applicant: Monsanto Technology, LLC

Inventor： Jonathan C. Lamb ,  Richard J. Lawrence

IPC: C12N15/82 ,  C12Q1/6895 ,  C12N9/22

Abstract: The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.

公开(公告)号：US20190169596A1

公开(公告)日：2019-06-06

申请号：US16275200

申请日：2019-02-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Charles L. ARMSTRONG ,  Edward J. Cargill ,  Fenggao Dong ,  Jonathan C. Lamb ,  Huachun W. Larue ,  Richard J. Lawrence ,  Thomas S. Ream

IPC: C12N15/10 ,  C12N9/22 ,  C12N9/12

Abstract: Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

公开(公告)号：US20150167010A1

公开(公告)日：2015-06-18

申请号：US14209828

申请日：2014-03-13

Applicant: Monsanto Technology, LLC

Inventor： Jonathan C. Lamb ,  Richard J. Lawrence

IPC: C12N15/82 ,  C12Q1/68

CPC classification number: C12N15/8213 ,  C12N9/22 ,  C12Q1/6895

Abstract: The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.

Abstract translation: 本发明涉及生物技术，并提供用于序列特异性或序列定向的转录激活子样效应物重组酶介导的目的DNA序列整合到宿主基因组中的新方法。 本发明还提供了用于新型植物转化载体和表达盒的方法，其包括嵌合重组酶与植物表达和转化元件的新型组合。 还提供了基因靶向，DNA序列去除，基因组修饰和分子育种的方法。

公开(公告)号：US20240124884A1

公开(公告)日：2024-04-18

申请号：US18538893

申请日：2023-12-13

Applicant: Monsanto Technology LLC

Inventor： Stanton B. Dotson ,  Larry A. Gilbertson ,  Jonathan C. Lamb ,  Brenda Lowe ,  Zoe P. McCuddin

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8257 ,  C12N15/8261 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12N15/8289 ,  C12N15/829

Abstract: The invention provides novel uses of sequence-specific or sequence-directed endonucleases for molecular plant breeding. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of an endonuclease with plant expression and transformation elements. Plants and derivatives thereof produced by such methods are also provided.