公开(公告)号：US20210371818A1

公开(公告)日：2021-12-02

申请号：US17348174

申请日：2021-06-15

Applicant: Osaka University

Inventor： Kohji NISHIDA ,  Ryuhei HAYASHI ,  Yuki ISHIKAWA

IPC: C12N5/079 ,  C12N5/10 ,  C12N5/074

Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.

公开(公告)号：US20210285911A1

公开(公告)日：2021-09-16

申请号：US17258662

申请日：2019-07-18

Applicant: OSAKA UNIVERSITY

Inventor： Kohji NISHIDA ,  Noriyasu HASHIDA ,  Masateru TANIGUCHI ,  Makusu TSUTSUI

IPC: G01N27/327 ,  G01N27/447 ,  G01N33/569

Abstract: Provided are a virus measuring method, a virus measuring device, a virus determining program, a stress determining method, and a stress determining device. A virus measuring method includes a contact step of bringing a liquid specimen containing a body fluid of a subject and an electrolytic solution into contact with each other via a through-hole portion formed in a separating wall, a current measuring step of applying a voltage to the liquid specimen and the electrolytic solution with respect to the through-hole portion and obtaining a waveform of an ionic current flowing through the through-hole portion, and a virus determining step of determining the kind of a virus contained in the body fluid on the basis of the waveform. In the virus determining step, the kind of the virus is determined by comparing the waveform with waveform information that corresponds to a known virus and is obtained beforehand.

公开(公告)号：US20210046122A1

公开(公告)日：2021-02-18

申请号：US16961354

申请日：2019-01-11

Applicant: OSAKA UNIVERSITY ,  ROHTO PHARMACEUTICAL CO., LTD.

Inventor： Kohji NISHIDA ,  Ryuhei HAYASHI ,  Shun SHIBATA ,  Toru OKUBO ,  Yoichi HONMA

IPC: A61K35/28 ,  A61P27/02

Abstract: The present invention addresses the problem of providing a novel therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The present invention is an agent for promoting normal differentiation/maturation of stratified squamous epithelial cells, which comprises a secretion of mesenchymal stem cells. It is preferable that the secretions not include animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and that the stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells, epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.

公开(公告)号：US20190015452A1

公开(公告)日：2019-01-17

申请号：US15749766

申请日：2016-08-03

Applicant: OSAKA UNIVERSITY ,  ROHTO PHARMACEUTICAL CO., LTD.

Inventor： Kohji NISHIDA ,  Ryuuhei HAYASHI ,  Yoichi HONMA ,  Toru OKUBO ,  Shun SHIBATA

IPC: A61K35/28 ,  A61P27/02 ,  C12N5/0775 ,  C12N5/00

Abstract: The present invention relates to mesenchymal stem cell-derived microparticles having activity that promotes the growth of corneal epithelial stem cells and/or corneal epithelial cells, activity that maintains corneal epithelial stem cells in an undifferentiated state or promotes the formation of colonies thereby, and function that protects corneal epithelium.