公开(公告)号：US10378043B2

公开(公告)日：2019-08-13

申请号：US15401291

申请日：2017-01-09

Applicant: QVELLA CORPORATION

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

IPC: C12Q1/6806 ,  C12M1/42 ,  C12M1/00 ,  C12N1/06 ,  C12N1/08 ,  C12N13/00 ,  B01L3/00 ,  C12Q3/00 ,  B01L7/00 ,  C12Q1/686

Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

公开(公告)号：US20150276727A1

公开(公告)日：2015-10-01

申请号：US14735917

申请日：2015-06-10

Applicant: QVELLA CORPORATION

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Stephen W. Leonard ,  Robert Maaskant ,  Tino Alavie

IPC: G01N33/543 ,  B01L3/00

CPC classification number: G01N33/5438 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0809 ,  C12M23/16 ,  C12M25/00 ,  C12M25/04 ,  C12M29/10 ,  C12M35/02 ,  C12M41/38 ,  C12N1/066 ,  C12N11/14 ,  C12N13/00 ,  G01N33/5005

Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Abstract translation: 本发明提供一种微流体装置及其用于浓缩和捕获细胞的方法。 在层流下相对于样品施加脉冲非法拉第电场，这导致带电分析物的浓度和捕获。 有利地，选择脉冲定时以避免与通道内的离子屏蔽相关的问题。 通道内的至少一个电极涂有绝缘层，以防止法拉第电流在通道中流动。 在脉冲施加单极电压给电极的情况下，样品内的带电分析物经由瞬时电泳力朝向其中一个电极移动。