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    METHODS OF TARGETED ANTIBIOTIC SUSCEPTIBILITY TESTING
    2.
    发明申请
    METHODS OF TARGETED ANTIBIOTIC SUSCEPTIBILITY TESTING 审中-公开

    公开(公告)号：US20190177766A1

    公开(公告)日：2019-06-13

    申请号：US16280842

    申请日：2019-02-20

    Applicant: Qvella Corporation

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

    IPC: C12Q1/18 ,  C12Q1/689

    Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

    METHODS OF TARGETED ANTIBIOTIC SUSCEPTIBILITY TESTING
    4.
    发明申请
    METHODS OF TARGETED ANTIBIOTIC SUSCEPTIBILITY TESTING 审中-公开
    靶向抗生素耐药性测试方法

    公开(公告)号：US20160138072A1

    公开(公告)日：2016-05-19

    申请号：US14901200

    申请日：2014-07-03

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

    IPC: C12Q1/18 ,  C12Q1/68

    CPC classification number: C12Q1/18 ,  C12Q1/689 ,  C12Q2600/106 ,  C12Q2600/158

    Abstract: Methods are provided for performing antibotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the pressence of a selected antibiotic and a stimulus is provided to induce the production on m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

    Abstract translation: 提供了基于在暴露于抗生素后从微生物细胞中检测RNA（例如tmRNA）进行抗生素敏感性测试的方法。 在一些实施方案中，从样品获得等分试样，其中一个样品含有选定的抗生素。 包括生长培养基在内的等分试样在适合于微生物生长的条件下孵育，并除去每个等分试样中的微生物细胞并裂解，并将裂解物进行逆转录和扩增，推断所选抗生素对微生物的作用 细胞。 在一个实施方案中，将含有微生物细胞的样品在所选择的抗生素的压力下孵育，并提供刺激以诱导在微生物细胞内的m RNA上产生。 随后裂解微生物细胞，而不会在裂解物中明显降解m RNA，并检测m RNA以确定抗生素对微生物细胞的作用。

    Method for pretreatment of microbial samples
    5.
    发明授权
    Method for pretreatment of microbial samples 有权
    微生物样品预处理方法

    公开(公告)号：US08975060B2

    公开(公告)日：2015-03-10

    申请号：US13833872

    申请日：2013-03-15

    Applicant: Qvella Corporation

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

    IPC: C12N1/00

    CPC classification number: C12Q1/6848 ,  B01L3/5021 ,  B01L3/50215 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1833 ,  C12Q1/02 ,  C12Q1/6806 ,  C12Q2523/307 ,  C12Q2527/125 ,  C12Q2527/137

    Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included, for collecting the microbial cells adjacent to a liquid interface formed by the cushioning liquid, upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed to prior to extraction of the pretreated sample.

    Abstract translation: 提供了用于预处理含有微生物细胞的样品的方法和装置。 在一些实施方案中，样品的预处理通过在样品预处理容器内，非微生物细胞（例如血细胞）中的初始选择性裂解进行，随后将样品离心以除去所得碎片并浓缩微生物 细胞。 可以包括不混溶和致密的缓冲液体，用于在预处理容器离心时收集由缓冲液形成的液体界面附近的微生物细胞。 在除去大量的上清液后，重新悬浮收集的微生物细胞并重新建立液体界面，可以除去至少一部分剩余的悬浮液，而基本不除去缓冲液体。 可以在提取预处理样品之前进行一个或多个中间洗涤循环。

    Methods of targeted antibiotic susceptibility testing
    6.
    发明授权
    Methods of targeted antibiotic susceptibility testing 有权

    公开(公告)号：US11866762B2

    公开(公告)日：2024-01-09

    申请号：US17240093

    申请日：2021-04-26

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

    IPC: C12Q1/18 ,  C12Q1/689

    CPC classification number: C12Q1/18 ,  C12Q1/689 ,  C12Q2600/106 ,  C12Q2600/158

    Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

    METHODS OF TARGETED ANTIBIOTIC SUSCEPTIBILITY TESTING
    7.
    发明申请
    METHODS OF TARGETED ANTIBIOTIC SUSCEPTIBILITY TESTING 有权

    公开(公告)号：US20210246483A1

    公开(公告)日：2021-08-12

    申请号：US17240093

    申请日：2021-04-26

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

    IPC: C12Q1/18 ,  C12Q1/689

    Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

    Methods of targeted antibiotic susceptibility testing
    8.
    发明授权
    Methods of targeted antibiotic susceptibility testing 有权

    公开(公告)号：US10988794B2

    公开(公告)日：2021-04-27

    申请号：US16280842

    申请日：2019-02-20

    Applicant: Qvella Corporation

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

    IPC: C12Q1/18 ,  C12Q1/68 ,  C12Q1/689

    Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

    Method for pretreatment of microbial cells
    10.
    发明授权
    Method for pretreatment of microbial cells 有权

    公开(公告)号：US11702647B2

    公开(公告)日：2023-07-18

    申请号：US16881479

    申请日：2020-05-22

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

    IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6806 ,  C12Q1/24 ,  C08F220/28

    CPC classification number: C12N15/1003 ,  B01L3/5021 ,  B01L3/5027 ,  C08F220/286 ,  C12Q1/24 ,  C12Q1/6806 ,  C08G2261/1452

    Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.

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