公开(公告)号：US11738343B2

公开(公告)日：2023-08-29

申请号：US17338856

申请日：2021-06-04

Applicant: QVELLA CORPORATION

Inventor： Robert Maaskant ,  Sanjesh Yasotharan ,  Samad Talebpour ,  Stephen W. Leonard ,  Cyrus Etemad-Moghadam ,  Alexander Zahn

IPC: B01L3/00 ,  B01L7/00 ,  B04B5/04 ,  B04B13/00 ,  B01D19/00 ,  B01D17/02 ,  F16K7/00 ,  B01D21/26 ,  C12N15/00 ,  F16K99/00 ,  C12N15/10

CPC classification number: B01L3/502753 ,  B01D17/0217 ,  B01D19/0052 ,  B01D21/262 ,  B01L3/5021 ,  B01L3/502715 ,  B01L3/502738 ,  B01L7/52 ,  B04B5/0407 ,  B04B5/0421 ,  B04B13/00 ,  C12N15/1003 ,  F16K7/00 ,  F16K99/0015 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/0655 ,  F16K2099/0084

Abstract: Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the microfluidic device. The integrated fluidic processing cartridge may include a supernatant chamber the extraction of a supernatant thereto, and a diluent chamber for diluting a suspension collected in the centrifugation chamber.

公开(公告)号：US11027279B2

公开(公告)日：2021-06-08

申请号：US16818095

申请日：2020-03-13

Applicant: QVELLA CORPORATION

Inventor： Robert Maaskant ,  Sanjesh Yasotharan ,  Samad Talebpour ,  Stephen W. Leonard ,  Cyrus Etemad-Moghadam ,  Alexander Zahn

IPC: B01L3/00 ,  B01L7/00 ,  B04B5/04 ,  B04B13/00 ,  B01D19/00 ,  B01D17/02 ,  F16K7/00 ,  B01D21/26 ,  C12N15/10 ,  F16K99/00

Abstract: Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the micro-fluidic device. The integrated fluidic processing cartridge may include a supernatant chamber the extraction of a supernatant thereto, and a dilutent chamber for diluting a suspension collected in the centrifugation chamber.

公开(公告)号：US10596570B2

公开(公告)日：2020-03-24

申请号：US15311732

申请日：2015-05-19

Applicant: QVELLA CORPORATION

Inventor： Robert Maaskant ,  Sanjesh Yasotharan ,  Samad Talebpour ,  Stephen W. Leonard ,  Cyrus Etemad-Moghadam ,  Alexander Zahn

IPC: B01D21/26 ,  B01D17/02 ,  B01D19/00 ,  B04B5/04 ,  B04B13/00 ,  C12N15/10 ,  F16K7/00 ,  F16K99/00 ,  B01L3/00 ,  B01L7/00

Abstract: Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the microfluidic device. The integrated fluidic processing cartridge may include a supernatant chamber the extraction of a supernatant thereto, and a diluent chamber for diluting a suspension collected in the centrifugation chamber.

公开(公告)号：US20150276727A1

公开(公告)日：2015-10-01

申请号：US14735917

申请日：2015-06-10

Applicant: QVELLA CORPORATION

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Stephen W. Leonard ,  Robert Maaskant ,  Tino Alavie

IPC: G01N33/543 ,  B01L3/00

CPC classification number: G01N33/5438 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0809 ,  C12M23/16 ,  C12M25/00 ,  C12M25/04 ,  C12M29/10 ,  C12M35/02 ,  C12M41/38 ,  C12N1/066 ,  C12N11/14 ,  C12N13/00 ,  G01N33/5005

Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Abstract translation: 本发明提供一种微流体装置及其用于浓缩和捕获细胞的方法。 在层流下相对于样品施加脉冲非法拉第电场，这导致带电分析物的浓度和捕获。 有利地，选择脉冲定时以避免与通道内的离子屏蔽相关的问题。 通道内的至少一个电极涂有绝缘层，以防止法拉第电流在通道中流动。 在脉冲施加单极电压给电极的情况下，样品内的带电分析物经由瞬时电泳力朝向其中一个电极移动。