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公开(公告)号：US20080060096A1
公开(公告)日：2008-03-06
申请号：US10569064
申请日：2004-07-31
申请人： Matt Sauer , Christel Renate Schopfer , Ralf Flachmann , Karin Herbers , Irene Kunze , Martin Klebsattel , Thomas Luck , Dirk Voeste , Angelika-Maria Pfeiffer , Hendrik Tschoep
发明人： Matt Sauer , Christel Renate Schopfer , Ralf Flachmann , Karin Herbers , Irene Kunze , Martin Klebsattel , Thomas Luck , Dirk Voeste , Angelika-Maria Pfeiffer , Hendrik Tschoep
CPC分类号： C12N9/0069 , A23K20/179 , A23L33/105 , C12N9/0004 , C12N15/8218 , C12N15/825 , C12P23/00
摘要： The present invention relates to a process for preparing ketocarotenoids by cultivating genetically modified, non-human organisms which have, by comparison with the wild type, a modified ketolase activity, to the genetically modified organisms, to the use thereof as human and animal foods and for preparing ketocarotenoid extracts, and to novel ketolases and nucleic acids encoding these ketolases.
摘要翻译： 本发明涉及一种通过培养经遗传修饰的非人类生物制备酮类胡萝卜素的方法，所述生物体与野生型相比，改性的酮糖酶活性与转基因生物有关，作为人和动物食品的用途，以及 用于制备酮类胡萝卜素提取物，以及新型酮糖酶和编码这些酮糖酶的核酸。
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12.发明申请Transgenic expression cassettes for expressing nucleic acids in non-reproductive floral tissues of plants 有权用于在植物的非生殖花卉组织中表达核酸的转基因表达盒公开(公告)号：US20060168695A1
公开(公告)日：2006-07-27
申请号：US10526411
申请日：2003-08-29
申请人： Martin Klebsattel , Ulrich Keetman , Karin Herbers , Ralf Flachmann , Matt Sauer , Heike Hillebrand
发明人： Martin Klebsattel , Ulrich Keetman , Karin Herbers , Ralf Flachmann , Matt Sauer , Heike Hillebrand
CPC分类号： C12N15/8223 , C12N15/8243 , C12N15/8257
摘要： The invention relates to methods for the specific, transgenic expression of nucleic acid sequences in non-reproductive flower tissues of plants, and expression cassettes and expression vectors containing promoters which have an expression specificity for non-productive tissues of the flower. The invention also relates to organisms (preferably plants), cultures, parts, or reproduction material derived therefrom, which are transformed by means of said transgenic expression cassettes or expression vectors, and the use thereof for producing food, fodder, seeds, pharmaceuticals, or fine chemicals.
摘要翻译： 本发明涉及用于植物非生殖花组织中核酸序列的特异性，转基因表达的方法，以及含有对花的非生产性组织具有表达特异性的启动子的表达盒和表达载体。 本发明还涉及通过所述转基因表达盒或表达载体转化的生物（优选植物），由其衍生的培养物，部分或繁殖材料及其用于生产食物，饲料，种子，药物或 精细化学品。
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13.发明授权公开(公告)号：US07385123B2
公开(公告)日：2008-06-10
申请号：US10524827
申请日：2003-08-18
CPC分类号： C12N9/0069 , A23K10/30 , A23K20/179 , A23K50/80 , A23L5/44 , A23L33/155 , A23V2002/00 , C07H21/00 , C09B61/00 , C12N9/0004 , C12N15/823 , C12N15/8243 , C12N15/825 , C12P23/00 , Y02A40/818 , A23V2300/21
摘要： The present invention relates to a process for preparing ketocarotenoids by cultivation of genetically modified organisms which, compared with the wild type, have a modified ketolase activity, to the genetically modified organisms, and to the use thereof as human and animal foods and for producing ketocarotenoid extracts.
摘要翻译： 本发明涉及通过种植转基因生物制备酮类胡萝卜素的方法，与野生型相比，具有改性的酮糖酶活性，转基因生物，以及作为人和动物食品的用途以及用于生产酮类胡萝卜素 提取物。
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14.发明授权Methods for producing animal feed preparations with astaxanthin-containing plants or parts of plants of the genus Tagetes 失效用于生产含有虾青素的植物或Tagetes属植物的部分的动物饲料制剂的方法公开(公告)号：US07381541B2
公开(公告)日：2008-06-03
申请号：US10524647
申请日：2003-08-18
申请人： Ralf Flachmann , Matt Sauer , Christel Renate Schopfer , Martin Klebsattel , Angelika-Maria Pfeiffer , Thomas Luck , Dirk Voeste
发明人： Ralf Flachmann , Matt Sauer , Christel Renate Schopfer , Martin Klebsattel , Angelika-Maria Pfeiffer , Thomas Luck , Dirk Voeste
IPC分类号： C12P23/00
CPC分类号： C12N9/0069 , A23K10/30 , A23K20/179 , A23K50/80 , A23L5/44 , A23L33/155 , A23V2002/00 , C07H21/00 , C09B61/00 , C12N9/0004 , C12N15/823 , C12N15/8243 , C12N15/825 , C12P23/00 , Y02A40/818 , A23V2300/21
摘要： The present invention relates to the use of astaxanthin-containing plants or parts of plants of the genus Tagetes or astaxanthin-containing extracts of astaxanthin-containing plants or parts of plants of the genus Tagetes for oral administration to animals, methods for producing animal feed preparations, the animal feed preparations themselves, a method for pigmenting animals or animal products, and also a method for producing pigmented animals and animal products.
摘要翻译： 本发明涉及含有虾青素的植物或含有虾青素的植物或含有虾青素的植物或属于Tagetes植物部分的植物的部分用于动物口服给药的用途，用于生产动物饲料制剂的方法 ，动物饲料制剂本身，着色动物或动物产品的方法，以及生产有色动物和动物产品的方法。
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15.发明授权Transgenic expression cassettes for expressing nucleic acids in non-reproductive floral tissues of plants 有权用于在植物的非生殖花卉组织中表达核酸的转基因表达盒公开(公告)号：US07402733B2
公开(公告)日：2008-07-22
申请号：US10526411
申请日：2003-08-29
申请人： Martin Klebsattel , Ulrich Keetman , Karin Herbers , Ralf Flachmann , Matt Sauer , Heike Hillebrand
发明人： Martin Klebsattel , Ulrich Keetman , Karin Herbers , Ralf Flachmann , Matt Sauer , Heike Hillebrand
CPC分类号： C12N15/8223 , C12N15/8243 , C12N15/8257
摘要： The invention relates to methods for the targeted transgenic expression of nucleic acid sequences in nonreproductive floral tissues of plants, and to transgenic expression cassettes and expression vectors which comprise promoters having an expression specificity for nonreproductive tissues of the flower. The invention further relates to organisms (preferably plants) transformed with these transgenic expression cassettes or expression vectors, to cultures, parts or propagation material derived therefrom, and to the use of the same for producing human and animal foods, seeds, pharmaceuticals or fine chemicals.
摘要翻译： 本发明涉及用于植物非生殖性花卉组织中核酸序列的靶向转基因表达的方法，以及包含对花的非生殖性组织具有表达特异性的启动子的转基因表达盒和表达载体的方法。 本发明进一步涉及用这些转基因表达盒或表达载体转化的生物体（优选植物），由其衍生的培养物，部分或繁殖材料，以及其用于生产人和动物食品，种子，药物或精细化学品的用途 。
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16.发明申请Transgenic expression cartridges for expressing nucleic acids in the flower tissue of plants 审中-公开用于在植物花组织中表达核酸的转基因表达盒公开(公告)号：US20090068163A1
公开(公告)日：2009-03-12
申请号：US11919862
申请日：2006-05-03
IPC分类号： A61K8/43 , C12N15/82 , C12Q1/68 , A01H5/00 , A61K31/70 , A61K31/20 , C12N1/21 , C12N1/19 , C12N1/15
CPC分类号： C12N15/823 , C07K14/415
摘要： The invention relates to methods for the targeted transgenic expression of nucleic acid sequences in tissues of plants, and to transgenic expression cassettes and expression vectors comprising promoters with expression specificity for floral tissues. The invention further relates to organisms (preferably plants) transformed with these transgenic expression cassettes or expression vectors, to cultures, parts or propagation material derived from these organisms, and to their use for the production of foodstuffs, feedstuffs, seed, pharmaceuticals or fine chemicals.
摘要翻译： 本发明涉及用于植物组织中核酸序列的靶向转基因表达的方法，以及包含对花组织具有表达特异性的启动子的转基因表达盒和表达载体的方法。 本发明还涉及用这些转基因表达盒或表达载体转化的生物体（优选植物），衍生自这些生物体的培养物，部分或繁殖材料及其用于生产食品，饲料，种子，药物或精细化学品的用途 。
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公开(公告)号：US20100005541A1
公开(公告)日：2010-01-07
申请号：US12514676
申请日：2007-11-13
申请人： George Mather Sauer , Ralf Flachmann , Christel Renate Schopfer , Michael Leps , Hannia Bridg-Giannakopoulos
发明人： George Mather Sauer , Ralf Flachmann , Christel Renate Schopfer , Michael Leps , Hannia Bridg-Giannakopoulos
CPC分类号： C12N15/825 , A01H1/02 , A01H1/06 , A01H5/02 , A23K20/179 , A23K50/80 , C12N9/0069 , C12N9/1022 , C12N15/823 , C12P23/00
摘要： New ketolases for the production of ketocarotenoids in Tagetes Abstract A ketolase having a polypeptide sequence SEQ ID NO:6, a sequence derived from SEQ ID NO:6 by substitution, insertion or deletion of aminoacids, which has an identity of at least 60% with the respective sequence SEQ. ID. NO: 6 or a functionally equivalent fragment of the sequences mentioned above and processes for production of ketocarotenoids in Tagetes.
摘要翻译： 用于生产Tagetes中的酮类胡萝卜素的新型酮酶本文摘要具有多肽序列SEQ ID NO：6的酮糖酶，通过取代，插入或缺失氨基酸得到的序列SEQ ID NO：6，其同一性至少为60％ 相应的序列SEQ。 ID。 NO：6或上述序列的功能等同片段和用于生产Tagetes中的酮类胡萝卜素的方法。
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18.发明申请Use of A Plastid-Lipid Associated Protein Promoter (PAP Promoter) For Heterologous Gene Expression 审中-公开用于异源基因表达的质体 - 脂质相关蛋白启动子（PAP启动子）的使用公开(公告)号：US20100021962A1
公开(公告)日：2010-01-28
申请号：US12304424
申请日：2007-06-12
CPC分类号： C12P23/00
摘要： Use of a plastid-lipid associated protein promoter (PAP promoter) for heterologous expression of genes in plants of the genus Tagetes.
摘要翻译： 使用质体 - 脂质相关蛋白启动子（PAP启动子）用于在Tagetes属植物中异源表达基因。
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公开(公告)号：US20120156718A1
公开(公告)日：2012-06-21
申请号：US13140218
申请日：2009-12-08
申请人： Ralf Flachmann , Irina Wenderoth , Christel Renate Schopter , Bettina Tschiersch , Hannia Bridg-Giannakopoulos , Michael Leps , Ute Linemann , George Mather Sauer
发明人： Ralf Flachmann , Irina Wenderoth , Christel Renate Schopter , Bettina Tschiersch , Hannia Bridg-Giannakopoulos , Michael Leps , Ute Linemann , George Mather Sauer
CPC分类号： C12N15/825 , C12N9/88
摘要： The present invention relates to optimized ketolase coding sequences, corresponding coding sequences and genetic constructs alone or in combination with beta-cyclase coding sequences, their use for the expression in plants, in particular in plants of the genus Tagetes, to such genetically modified plants, to a process for the preparation of carotenoid products by culturing the genetically modified plants. The present invention further relates to the expression of optimized ketolase coding sequences alone or in combination with beta-cyclase coding sequences under control of an Antirrhinum majus ANTHIRRHINUM FIDDLEHEAD (AFI) promoter, In addition the invention relates to the use of an AFI promoter for heterologous gene expression, preferably for flower-specific expression of genes in plants of the genus Tagetes, to the genetically modified plants of the genus Tagetes, and to a process for producing biosynthetic products by cultivating the genetically modified plants.
摘要翻译： 本发明涉及优化的酮醇酶编码序列，相应的编码序列和单独的或与β-环化酶编码序列组合的遗传构建体，它们在植物中，特别是在加泰米松属植物中的表达用于此类转基因植物， 涉及通过培养转基因植物来制备类胡萝卜素产物的方法。 本发明还涉及在金鱼草ANTHIRRININ FIDDHEAD（AFI）启动子的控制下单独或与β-环化酶编码序列组合的优化的酮醇酶编码序列的表达。此外，本发明涉及AFI启动子在异源 基因表达，优选用于花旗特异性表达的Tagetes植物中的基因，转基因植物的Tagetes，以及通过培养转基因植物生产生物合成产物的方法。
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