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    Method for serological detection of viral antigens
    13.
    发明授权
    Method for serological detection of viral antigens 有权

    公开(公告)号：US11150247B2

    公开(公告)日：2021-10-19

    申请号：US16204580

    申请日：2018-11-29

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Ralf Bollhagen ,  Barbara Upmeier ,  Toralf Zarnt ,  Peter Muench ,  Manfred Ginter

    IPC: G01N33/576 ,  G01N33/53

    Abstract: The present disclosure relates to a method for detecting a core polypeptide of a hepatitis C virus (HCV) in a sample from a subject involving (a) contacting said sample with a base and with a surfactant having a cationic detergent, and (b) detecting a core polypeptide of the HCV in the sample. The present invention further relates to a method for pre-processing a sample from a subject for detection of HCV, involving contacting the sample with a base and with a surfactant having a cationic detergent; and to a pre-processing reagent for detecting HCV in a sample, having a base and a surfactant including a cationic detergent, wherein the surfactant also has a nonionic detergent. Moreover, the present disclosure further relates to kits, uses, and devices related to the methods disclosed.

    SOLUBLE AND IMMUNOREACTIVE FLAVIVIRAL NS1 POLYPEPTIDES
    14.
    发明申请
    SOLUBLE AND IMMUNOREACTIVE FLAVIVIRAL NS1 POLYPEPTIDES 审中-公开

    公开(公告)号：US20200048313A1

    公开(公告)日：2020-02-13

    申请号：US16663457

    申请日：2019-10-25

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Elke Faatz ,  Alexander Riedel ,  Christian Scholz ,  Peter Muench ,  Gloria Tabares ,  Mario Gloeck ,  Silke Luebcke ,  Juliane Benz

    IPC: C07K14/18 ,  G01N33/569 ,  C12N7/00

    Abstract: The disclosure relates to a polypeptide suitable for detecting antibodies against a flavivirus in an isolated biological sample having a flavivirus NS1 wing domain specific amino acid sequence, wherein no amino acid sequences from the NS1 ß-ladder domain of said flavivirus are present in the polypeptide. In an embodiment, the flavivirus is selected from Zika virus (ZIKV), West-Nile virus (WNV), Dengue virus types 1-4 (DENV1-4), tick-borne encephalitis virus (TBEV), yellow fever virus (YFV) and Japanese encephalitis virus (JEV). Also disclosed is a method for producing said flaviviral NS1 wing domain specific polypeptides, a method for detecting antibodies specific for a first flavivirus species, the use of said flaviviral NS1 wing domain specific polypeptides for detecting antibodies as well as a reagent kit for detecting said flavivirus antibodies that has a flavivirus NS1 wing domain polypeptide.

    SOLUBLE AND IMMUNOREACTIVE ZIKA VIRUS NS1 POLYPEPTIDES
    15.
    发明申请
    SOLUBLE AND IMMUNOREACTIVE ZIKA VIRUS NS1 POLYPEPTIDES 审中-公开

    公开(公告)号：US20200048312A1

    公开(公告)日：2020-02-13

    申请号：US16663417

    申请日：2019-10-25

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Elke Faatz ,  Mario Gloeck ,  Peter Muench ,  Alexander Riedel ,  Christian Scholz ,  Gloria Tabares

    IPC: C07K14/005 ,  G01N33/569

    Abstract: The disclosure concerns a polypeptide suitable for detecting antibodies against Zika virus in an isolated biological sample having a Zika virus NS1 wing domain specific amino acid sequence and variants thereof, wherein no further Zika virus specific amino acid sequences are present in the polypeptide. This polypeptide does not immunologically cross-react with antibodies raised against structurally related antigens from tick-borne encephalitis virus, Dengue virus 1-4, West Nile virus, yellow fever virus or Japanese encephalitis virus, but immunologically reacts with antibodies raised against full length Zika virus NS1 antigen. Also disclosed is a method of producing a soluble and immunoreactive Zika virus NS1 polypeptide as well as methods and kits for detecting antibodies specific for Zika virus in an isolated sample.

    Soluble and immunoreactive variants of HTLV capsid antigen p24
    16.
    发明授权
    Soluble and immunoreactive variants of HTLV capsid antigen p24 有权

    公开(公告)号：US10466242B2

    公开(公告)日：2019-11-05

    申请号：US15246607

    申请日：2016-08-25

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Elke Faatz ,  Christian Scholz ,  Peter Muench

    IPC: A61K39/12 ,  G01N33/569 ,  C07K14/005

    Abstract: The invention concerns soluble and antigenic HTLV p24 variants that can be fused to chaperones and their use in diagnostic applications such as immunoassays for detecting antibodies against HTLV-I or HTLV-II in an isolated biological sample. In particular, the invention relates to a soluble HTLV-I or HTLV-II p24 antigen comprising either the N- or the C-terminal domain of p24 and lacking the other domain. Moreover, the invention covers recombinant DNA molecules encoding these HTLV-I and -II fusion antigens as well as their recombinant production using expression vectors and host cells transformed with such expression vectors. In addition, the invention focuses on compositions of these HTLV p24 antigens with HTLV gp21 antigen and on an immunoassay method for detection of HTLV antibodies using the antigens of the invention. Also the use of HTLV p24 antigens in an in vitro diagnostic assay as well as a reagent kit for detection of anti-HTLV-antibodies comprising said HTLV antigens is encompassed.

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