公开(公告)号：US20240417785A1

公开(公告)日：2024-12-19

申请号：US18615714

申请日：2024-03-25

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Mark Stamatios Kokoris ,  John Tabone ,  Melud Nabavi ,  Aaron Jacobs ,  Dylan O'Connell ,  Drew Goodman ,  Lacey Merrill ,  Jagadeeswaran Chandrasekar

IPC: C12Q1/6848 ,  C12Q1/6869

Abstract: The invention relates to methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

公开(公告)号：US20240191283A1

公开(公告)日：2024-06-13

申请号：US18348235

申请日：2023-07-06

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Robert M. McRuer ,  Mark Stamatios Kokoris

IPC: C12Q1/6825 ,  C12Q1/6869 ,  G01N27/447 ,  G01N33/487

CPC classification number: C12Q1/6825 ,  C12Q1/6869 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/631

Abstract: Translocation control for sensing by a nanopore, as well as methods and products related to the same, are provided. Such methods optimize duplex stability to provide high fill rate (of the hybridization sites) but do not prevent rapid dissociation required for high read rates, as well as controlling the translocation of a target molecule for sensing by a nanopore by use of a selective pulsed voltage. Products related to the same include a reporter construct comprising two or more phosphoramidites.