公开(公告)号：US11970731B2

公开(公告)日：2024-04-30

申请号：US16959985

申请日：2018-12-27

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Mark Stamatios Kokoris ,  John Tabone ,  Melud Nabavi ,  Aaron Jacobs ,  Dylan O'Connell ,  Drew Goodman ,  Lacey Merrill ,  Jagadeeswaran Chandrasekar ,  Kendall Berg ,  Samantha Vellucci ,  Jessica Vellucci

IPC: C12Q1/6848 ,  C07D249/06 ,  C07D401/14 ,  C07D403/14 ,  C07D471/04 ,  C12Q1/6869

CPC classification number: C12Q1/6848 ,  C07D249/06 ,  C07D401/14 ,  C07D403/14 ,  C07D471/04 ,  C12Q1/6869

Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

公开(公告)号：US20250075265A1

公开(公告)日：2025-03-06

申请号：US18604249

申请日：2024-03-13

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Mark Stamatios Kokoris ,  John Tabone ,  Melud Nabavi ,  Aaron Jacobs ,  Dylan O'Connell ,  Drew Goodman ,  Lacey Merrill ,  Jagadeeswaran Chandrasekar ,  Kendall Berg ,  Samantha Vellucci ,  Jessica Vellucci

IPC: C12Q1/6848 ,  C07D249/06 ,  C07D401/14 ,  C07D403/14 ,  C07D471/04 ,  C12Q1/6869

Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

公开(公告)号：US20240417785A1

公开(公告)日：2024-12-19

申请号：US18615714

申请日：2024-03-25

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Mark Stamatios Kokoris ,  John Tabone ,  Melud Nabavi ,  Aaron Jacobs ,  Dylan O'Connell ,  Drew Goodman ,  Lacey Merrill ,  Jagadeeswaran Chandrasekar

IPC: C12Q1/6848 ,  C12Q1/6869

Abstract: The invention relates to methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

公开(公告)号：US20240383938A1

公开(公告)日：2024-11-21

申请号：US18788229

申请日：2024-07-30

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Mark Kokoris ,  John Tabone ,  Melud Nabavi ,  Aaron Jacobs

IPC: C07H19/10 ,  C07F9/6574 ,  C07H19/14 ,  C07H23/00

Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.