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公开(公告)号：US20250019742A1
公开(公告)日：2025-01-16
申请号：US18420048
申请日：2024-01-23
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , Robert N. McRuer
IPC: C12Q1/6806 , C12Q1/6869 , G01N33/543
Abstract: Methods and related products are disclosed that improve the probability of interaction between a target molecule and a nanopore by capturing the target molecule on a surface comprising the nanopore. The captured target molecule, the nanopore, or both, are able to move relative to each other along the surface. When the leader of the target molecule is in proximity with the nanopore, interaction of the target portion of the target molecule with the nanopore occurs, thereby permitting sensing of the target portion. Confining the target molecule and nanopore in this manner leads to significantly enhanced interaction with the nanopore.
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公开(公告)号：US20250109449A1
公开(公告)日：2025-04-03
申请号：US18665321
申请日：2024-05-15
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , Robert N. McRuer
IPC: C12Q1/6897 , C07H21/00 , C12Q1/68 , C12Q1/6869 , C12Q1/6876
Abstract: Nucleic acid sequencing methods and related products are disclosed. Methods for sequencing a target nucleic acid comprise providing a daughter strand produced by a template-directed synthesis, the daughter strand comprising a plurality of subunits coupled in a sequence corresponding to a contiguous nucleotide sequence of all or a portion of the target nucleic acid, wherein the individual subunits comprise a tether, at least one probe or nucleobase residue, and at least one selectively cleavable bond. The selectively cleavable bond(s) is/are cleaved to yield an Xpandomer of a length longer than the plurality of the subunits of the daughter strand, the Xpandomer comprising the tethers and reporter elements for parsing genetic information in a sequence corresponding to the contiguous nucleotide sequence of all or a portion of the target nucleic acid. Reporter elements of the Xpandomer are then detected. Corresponding products, including Xpandomers are also disclosed.
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公开(公告)号：US11970731B2
公开(公告)日：2024-04-30
申请号：US16959985
申请日：2018-12-27
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , John Tabone , Melud Nabavi , Aaron Jacobs , Dylan O'Connell , Drew Goodman , Lacey Merrill , Jagadeeswaran Chandrasekar , Kendall Berg , Samantha Vellucci , Jessica Vellucci
IPC: C12Q1/6848 , C07D249/06 , C07D401/14 , C07D403/14 , C07D471/04 , C12Q1/6869
CPC classification number: C12Q1/6848 , C07D249/06 , C07D401/14 , C07D403/14 , C07D471/04 , C12Q1/6869
Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.
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公开(公告)号：US20250115895A1
公开(公告)日：2025-04-10
申请号：US18676209
申请日：2024-05-28
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , Marc Prindle , Melud Nabavi , Craig Ostrander , Taylor Lehmann , Samantha Vellucci , Michael Kovarik , Jack Chase , Robert Busam , Miranda Lahman
Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.
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公开(公告)号：US20240240161A1
公开(公告)日：2024-07-18
申请号：US18328078
申请日：2023-06-02
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , Marc Prindle , Jack Chase , Robert Busam , Michael Kovarik , Salka Keller , Megan Murt , Greg Thiessen
IPC: C12N9/12 , C12P19/34 , C12Q1/6806
CPC classification number: C12N9/1252 , C12P19/34 , C12Q1/6806 , C12Y207/07007
Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.
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Patent Agency Ranking
公开(公告)号：US12037577B2
公开(公告)日：2024-07-16
申请号：US17653441
申请日：2022-03-03
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , Marc Prindle , Melud Nabavi , Craig Ostrander , Taylor Lehmann , Samantha Vellucci , Michael Kovarik , Jack Chase , Robert Busam , Miranda Lahman
CPC classification number: C12N15/10 , C07H21/04 , C12N9/1252 , C12Y207/07007
Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.
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公开(公告)号：US20250002899A1
公开(公告)日：2025-01-02
申请号：US18825184
申请日：2024-09-05
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , Michael Kovarik , Marc Prindle , Salka Keller , Robert Busam , Miranda Lahman
Abstract: Methods and compositions for the selection of nucleic acid processing and other enzymes, and more specifically for the selection of DNA polymerases and other enzymes with desired properties employing the directed evolution of enzymes by plasmid tagging in droplets.
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公开(公告)号：US12116570B2
公开(公告)日：2024-10-15
申请号：US16337282
申请日：2017-09-28
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , Michael Kovarik , Marc Prindle , Salka Keller , Robert Busam , Miranda Lahman
CPC classification number: C12N15/1058 , C12N1/20 , C12N9/1252 , C12N9/93 , C12N15/74 , C40B20/04 , C40B40/08 , C12Y207/07007
Abstract: Methods and compositions for the selection of nucleic acid processing and other enzymes, and more specifically for the selection of DNA polymerases and other enzymes with desired properties employing the directed evolution of enzymes by plasmid tagging in droplets.
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公开(公告)号：US11920184B2
公开(公告)日：2024-03-05
申请号：US17071778
申请日：2020-10-15
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , Robert N. McRuer
IPC: C12Q1/6806 , C12Q1/6869 , G01N33/543
CPC classification number: C12Q1/6806 , C12Q1/6869 , G01N33/54373 , C12Q1/6869 , C12Q2563/131 , C12Q2565/531 , C12Q2565/631
Abstract: Methods and related products are disclosed that improve the probability of interaction between a target molecule and a nanopore by capturing the target molecule on a surface comprising the nanopore. The captured target molecule, the nanopore, or both, are able to move relative to each other along the surface. When the leader of the target molecule is in proximity with the nanopore, interaction of the target portion of the target molecule with the nanopore occurs, thereby permitting sensing of the target portion. Confining the target molecule and nanopore in this manner leads to significantly enhanced interaction with the nanopore.
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公开(公告)号：US20250075265A1
公开(公告)日：2025-03-06
申请号：US18604249
申请日：2024-03-13
Applicant: Roche Sequencing Solutions, Inc.
Inventor： Mark Stamatios Kokoris , John Tabone , Melud Nabavi , Aaron Jacobs , Dylan O'Connell , Drew Goodman , Lacey Merrill , Jagadeeswaran Chandrasekar , Kendall Berg , Samantha Vellucci , Jessica Vellucci
IPC: C12Q1/6848 , C07D249/06 , C07D401/14 , C07D403/14 , C07D471/04 , C12Q1/6869
Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.
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