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    NONCOVALENT COLLAGEN CROSSLINKING AGENT
    12.
    发明申请
    NONCOVALENT COLLAGEN CROSSLINKING AGENT 失效
    非专利胶原蛋白交联剂

    公开(公告)号：US20110060129A1

    公开(公告)日：2011-03-10

    申请号：US12990897

    申请日：2009-05-01

    申请人： Mitsuru Akashi ,  Kohji Nishida ,  Michiya Matsusaki ,  Akira Kubota ,  Tomonori Waku

    发明人： Mitsuru Akashi ,  Kohji Nishida ,  Michiya Matsusaki ,  Akira Kubota ,  Tomonori Waku

    IPC分类号： C07K14/78

    CPC分类号： A61K38/39

    摘要： An object of the invention is to provide a collagen crosslinking agent superior in biocompatibility that is free from the damage by UV irradiation and also from the problems of toxicity caused by residual monomer or unreacted functional groups.[Means to Solve] Provided is a noncovalent collagen crosslinking agent (for fibrous protein collagen), comprising a spacer of a polyvalent alcohol having two or more OH groups at the terminals and arms of collagen peptides formed of repetitions of three amino acids, the arms being bound via the OH groups to the spacer.

    摘要翻译： 本发明的目的是提供一种无生物相容性优异的胶原交联剂，其不受紫外线照射的损伤，也可由残留单体或未反应官能团引起的毒性问题。 [解决方法]提供了一种非共价胶原交联剂（纤维蛋白胶原蛋白），其包含在末端具有两个或更多个OH基团的多元醇的间隔基和由重复的三个氨基酸形成的胶原肽的臂，臂 通过OH基团结合到间隔基上。

    Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same
    13.
    发明申请
    Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same 有权
    再生角膜内皮细胞片，其制造方法及其使用方法

    公开(公告)号：US20070148137A1

    公开(公告)日：2007-06-28

    申请号：US10546275

    申请日：2004-02-20

    申请人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

    发明人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

    IPC分类号： A61K35/30 ,  C12N5/08

    CPC分类号： A61L27/3808 ,  A61L27/3839 ,  A61L27/3895 ,  A61L27/50 ,  A61L2430/16 ,  C12N5/0621 ,  C12N2539/10

    摘要： An improved process for producing a regenerated corneal endothelial cell sheet, comprising the steps of allowing corneal endothelial cells collected from a tissue to be cultivated on a cell culture support having its surface covered with a polymer of which the hydrating force varies in a temperature range of 0-80° C., and after the culture, (1) adjusting the temperature of the culture solution to the temperature at which the polymer on the substrate surface is hydrated, (2) bringing the cultured corneal endothelial cell sheet into close contact with a carrier, and (3) detaching the sheet together with the carrier. The regenerated corneal endothelial cell sheet obtained by the process will adhere very well to living tissues.

    摘要翻译： 一种用于生产再生角膜内皮细胞片的改进方法，包括以下步骤：将从组织收集的角膜内皮细胞培养在细胞培养支持物上，所述细胞培养支持物的表面被聚合物覆盖，所述聚合物的水合力在温度范围 0-80℃，培养后，（1）将培养液的温度调节至基材表面的聚合物水合的温度，（2）使培养的角膜内皮细胞片与 载体，和（3）与载体一起分离片材。 通过该方法获得的再生角膜内皮细胞片材将很好地粘附到活组织。

    Cell sheets for ectocornea formation, method of producing the same and method of using the same
    14.
    发明申请
    Cell sheets for ectocornea formation, method of producing the same and method of using the same 审中-公开
    用于外胚叶形成的细胞片，其制造方法及其使用方法

    公开(公告)号：US20060234377A1

    公开(公告)日：2006-10-19

    申请号：US10544541

    申请日：2004-02-06

    申请人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

    发明人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

    IPC分类号： C12N5/08

    CPC分类号： A61L27/3891 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3895 ,  A61L2430/16 ,  C12N5/0621 ,  C12N2539/10

    摘要： The present invention has as its objective providing acorneal epithelium forming cell sheet that will adhere well to an anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0-80° C., optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.

    摘要翻译： 本发明的目的是提供能够很好地粘附于前段组织的上皮细胞上皮细胞片。 为了实现该目的，通过包括以下步骤的方法制备角膜上皮细胞片，该方法包括以下步骤：在细胞培养载体上在特定条件下培养角膜上皮形成细胞，所述细胞培养载体包含其表面覆盖有温度响应性聚合物的底物，所述温度响应性聚合物的水合力 在0-80℃的温度范围内变化，任选地分层培养细胞层，然后（1）将培养溶液的温度调节至高于上临界溶解温度或低于临界溶解温度以下， （2）使培养的角膜上皮形成细胞与载体紧密接触，和（3）在规定的条件下将载片与载体一起分离。