Abstract:
A treatment method for biomass which is a pretreatment method including continuously performing hydrothermal treatment using a screw extruder to produce a biomass composition for saccharification from cellulose-containing biomass serving as a raw material with a small amount of electricity. The treatment method sequentially includes: pulverizing, in a pulverization section of the screw extruder, the cellulose-containing biomass so as to have a maximum grain size of 1,000 μm or less and adjusting a water content ratio thereof to from 30% to 80%; performing, in a heating section of the screw extruder, hydrothermal treatment at a temperature of from 205° C. to 250° C. for 0.1 minute to 10 minutes while performing kneading pulverization having a grinding effect; and cooling, in a cooling section of the screw extruder downstream of the heating section, a treated product to 100° C. or less to recover the treated product.
Abstract:
A pretreatment method for producing sugar from a cellulose-containing biomass, which includes subjecting the cellulose-containing biomass to hydrothermal treatment while fluidizing the cellulose-containing biomass; a production method of a biomass composition for saccharification use, including the pretreatment method; and a method for producing sugar, which includes hydrolyzing the biomass composition for saccharification use obtained by the above-mentioned production method.
Abstract:
A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.