METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS
    11.
    发明申请
    METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS 审中-公开
    用于处理含细菌生物质的方法

    公开(公告)号:US20160243782A1

    公开(公告)日:2016-08-25

    申请号:US15027599

    申请日:2014-09-09

    Abstract: A treatment method for biomass which is a pretreatment method including continuously performing hydrothermal treatment using a screw extruder to produce a biomass composition for saccharification from cellulose-containing biomass serving as a raw material with a small amount of electricity. The treatment method sequentially includes: pulverizing, in a pulverization section of the screw extruder, the cellulose-containing biomass so as to have a maximum grain size of 1,000 μm or less and adjusting a water content ratio thereof to from 30% to 80%; performing, in a heating section of the screw extruder, hydrothermal treatment at a temperature of from 205° C. to 250° C. for 0.1 minute to 10 minutes while performing kneading pulverization having a grinding effect; and cooling, in a cooling section of the screw extruder downstream of the heating section, a treated product to 100° C. or less to recover the treated product.

    Abstract translation: 一种生物质处理方法,其特征在于,所述预处理方法包括使用螺杆挤出机连续进行水热处理,从而生成用于以少量电的原料的含纤维素生物质的糖化的生物质组合物。 处理方法依次包括:在螺杆挤出机的粉碎部分中,将含纤维素的生物质粉碎至最大粒径为1,000μm以下,并将其含水率调整为30%〜80%; 在螺杆挤出机的加热部分中,在进行具有研磨效果的捏合粉碎的同时,在205℃至250℃的温度下进行0.1分钟至10分钟的水热处理; 在加热部下游的螺杆挤出机的冷却部中将处理后的产物冷却至100℃以下,回收处理后的产物。

    MANUFACTURING METHOD FOR 1,4-BUTANEDIOL, MICROBE, AND GENE
    13.
    发明申请
    MANUFACTURING METHOD FOR 1,4-BUTANEDIOL, MICROBE, AND GENE 有权
    1,4-丁二醇,微生物和基因的制备方法

    公开(公告)号:US20150376657A1

    公开(公告)日:2015-12-31

    申请号:US14440116

    申请日:2013-11-28

    Abstract: A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.

    Abstract translation: 通过使用微生物和/或其培养物通过乙酰辅酶A,乙酰乙酰辅酶A,3-羟基丁酰辅酶A,巴豆酰辅酶A和4-羟基丁酰辅酶A制备1,4-丁二醇的方法,其中 1,4-丁二醇的制造方法包括(a)具有序列号1的碱基序列的基因中的任一基因,(b)具有缺失一个或多个碱基的碱基序列,取代基, 或以序列号1的碱基序列加入,其中所述基因具有相对于序列号1的碱基序列具有大于或等于90%的同一性的碱基序列,和(c)与基因杂交的基因 其具有与在严格条件下具有序列号1所述的碱基序列的基因互补的碱基序列,并且包括(d)具有序列号为2〜9的碱基序列的基因中的任何一个或多个基因,(e) 具有碱基序列使得一个或多个碱基被缺失,取代或添加的基因 序列号2〜9的碱基序列,其中所述基因具有相对于其原始碱基序列具有大于或等于90%的同一性的碱基序列,和(f)与具有与基因互补的碱基序列的基因杂交的基因, 在严格条件下具有序列号2到9的碱基序列。

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