Methods for degrading lignocellulosic materials
    14.
    发明授权
    Methods for degrading lignocellulosic materials 有权
    降解木质纤维素材料的方法

    公开(公告)号:US07943350B2

    公开(公告)日:2011-05-17

    申请号:US11821438

    申请日:2007-06-22

    IPC分类号: C12P19/04

    摘要: The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermenting microorganisms; and (c) recovering the organic substance from the fermentation.

    摘要翻译: 本发明涉及降解木质纤维素材料的方法,包括:在至少一种表面活性剂存在下,用有效量的一种或多种纤维素分解酶处理木质纤维素材料,所述表面活性剂选自仲醇乙氧基化物,脂肪醇乙氧基化物 ,壬基酚乙氧基化物,十三烷基乙氧基化物和聚氧乙烯醚,其中与不存在表面活性剂相比,表面活性剂的存在增加了木质纤维素材料的降解。 本发明还涉及生产有机物质的方法,其包括:(a)在至少一种表面活性剂存在下,用有效量的一种或多种纤维素分解酶对木质纤维素材料进行糖化,所述表面活性剂选自仲醇乙氧基化物 ,脂肪醇乙氧基化物,壬基酚乙氧基化物,十三烷基乙氧基化物和聚氧乙烯醚,其中与不存在表面活性剂相比,表面活性剂的存在增加了木质纤维素材料的降解; (b)用一种或多种发酵微生物发酵步骤(a)的糖化木质纤维素材料; 和(c)从发酵中回收有机物质。

    Methods for degrading lignocellulosic materials
    16.
    发明申请
    Methods for degrading lignocellulosic materials 有权
    降解木质纤维素材料的方法

    公开(公告)号:US20050164355A1

    公开(公告)日:2005-07-28

    申请号:US11036871

    申请日:2005-01-14

    摘要: The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermentating microoganisms; and (c) recovering the organic substance from the fermentation.

    摘要翻译: 本发明涉及降解木质纤维素材料的方法,包括:在至少一种表面活性剂存在下,用有效量的一种或多种纤维素分解酶处理木质纤维素材料,所述表面活性剂选自仲醇乙氧基化物,脂肪醇乙氧基化物 ,壬基酚乙氧基化物,十三烷基乙氧基化物和聚氧乙烯醚,其中与不存在表面活性剂相比,表面活性剂的存在增加了木质纤维素材料的降解。 本发明还涉及生产有机物质的方法,其包括:(a)在至少一种表面活性剂存在下,用有效量的一种或多种纤维素分解酶对木质纤维素材料进行糖化,所述表面活性剂选自仲醇乙氧基化物 ,脂肪醇乙氧基化物,壬基酚乙氧基化物,十三烷基乙氧基化物和聚氧乙烯醚,其中与不存在表面活性剂相比,表面活性剂的存在增加了木质纤维素材料的降解; (b)用一种或多种发酵微生物发酵步骤(a)的糖化木质纤维素材料; 和(c)从发酵中回收有机物质。

    Method for preparing maltogenic alpha-amylase variants
    18.
    发明申请
    Method for preparing maltogenic alpha-amylase variants 有权
    麦芽糖α-淀粉酶变体的制备方法

    公开(公告)号:US20090203108A1

    公开(公告)日:2009-08-13

    申请号:US12413758

    申请日:2009-03-30

    IPC分类号: C12N9/28

    CPC分类号: C12N9/2417 A21D2/26 A21D8/042

    摘要: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

    摘要翻译: 基于麦芽糖α-淀粉酶Novamyl的三维结构,本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质。 改变的pH最佳,改善的热稳定性,增加的比活性,改变的切割模式或增加降低淀粉退化或面包陈化的能力。

    Methods for degrading or converting plant cell wall polysaccharides
    20.
    发明申请
    Methods for degrading or converting plant cell wall polysaccharides 有权
    降解或转化植物细胞壁多糖的方法

    公开(公告)号:US20050233423A1

    公开(公告)日:2005-10-20

    申请号:US11078921

    申请日:2005-03-10

    摘要: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.

    摘要翻译: 本发明涉及用于将植物细胞壁多糖转化成一种或多种产品的方法,其包括:用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖,其中所述重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法,其特征在于,包括:(a)用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖,其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; (b)用一种或多种发酵微生物发酵步骤(a)的糖化物质; 和(c)从发酵中回收有机物质。