公开(公告)号：US20210388320A1

公开(公告)日：2021-12-16

申请号：US17386776

申请日：2021-07-28

Applicant: Osaka University

Inventor： Shigeo Masuda ,  Nagako Sougawa ,  Satsuki Fukushima ,  Shigeru Miyagawa ,  Yoshiki Sawa

IPC: C12N5/077 ,  C12N5/00 ,  C12N5/10 ,  A61K35/33

Abstract: Provided is a cell population comprising differentiated cells obtainable by inducing differentiation of pluripotent stem cells, wherein the content ratio of undifferentiated pluripotent stem cells is 0.2% or less.

公开(公告)号：US10696948B2

公开(公告)日：2020-06-30

申请号：US15511275

申请日：2015-09-14

Applicant: OSAKA UNIVERSITY

Inventor： Yukiko Ochi ,  Kiyotoshi Sekiguchi ,  Shigeru Miyagawa ,  Yoshiki Sawa ,  Antti Markus Siltanen

IPC: C12N5/077 ,  A61K35/34 ,  C12N5/074

Abstract: The present invention provides a method for preparing a clinically applicable, safe and less damaged cardiomyocyte population through a brief and simple procedure from a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes. The present invention relates to a method for preparing a cardiomyocyte population, the method comprising the steps of: (1) inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin α2β1γ1, laminin α2β2γ1, laminin α1β1γ1 and laminin α1β2γ1, or a fragment thereof having integrin binding activity, and (3) retrieving cells adherent to the laminin or the laminin fragment.

公开(公告)号：US09968716B2

公开(公告)日：2018-05-15

申请号：US15028803

申请日：2014-09-10

Applicant: Ono Pharmaceutical Co., Ltd. ,  Osaka University

Inventor： Yoshiki Sawa ,  Toru Kuratani ,  Koichi Toda ,  Takayoshi Ueno ,  Shigeru Miyagawa ,  Satsuki Fukushima ,  Atsuhiro Saito ,  Yoshiki Watanabe ,  Yoshiki Sakai

IPC: A61L31/16 ,  A61F2/07 ,  A61L31/14 ,  A61F2/82 ,  A61K47/34 ,  A61K47/36 ,  A61K47/42 ,  A61K31/343 ,  A61K31/4406 ,  A61K31/5585 ,  A61K9/00

CPC classification number: A61L31/16 ,  A61F2/07 ,  A61F2/82 ,  A61F2240/001 ,  A61F2250/0067 ,  A61F2310/0097 ,  A61K9/0024 ,  A61K31/343 ,  A61K31/4406 ,  A61K31/5585 ,  A61K47/34 ,  A61K47/36 ,  A61K47/42 ,  A61L31/148 ,  A61L2300/412 ,  A61L2300/602

Abstract: The present invention provides a drug-eluting stent graft for preventing stent graft-related complications as well as treating aneurysm. Specifically, the present invention relates to a drug-eluting stent graft comprising a drug, a drug-retaining agent and a stent graft, and a method for therapy of aneurysm including the use of the drug-eluting stent graft.

公开(公告)号：US09623078B2

公开(公告)日：2017-04-18

申请号：US14436906

申请日：2013-10-24

Applicant: GENOMIX CO., LTD ,  OSAKA UNIVERSITY

Inventor： Katsuto Tamai ,  Sokichi Kamata ,  Yasufumi Kaneda ,  Shigeru Miyagawa ,  Yoshiki Sawa ,  Takehiko Yamazaki

IPC: A61K38/17 ,  C07K14/47

CPC classification number: A61K38/1709 ,  C07K14/4718

Abstract: A fragment peptide having a proper length composed of a part of an HMGB1 protein was synthesized and the peptide was confirmed to exhibit therapeutic effects on myocardial infarction.

公开(公告)号：US11441115B2

公开(公告)日：2022-09-13

申请号：US17042301

申请日：2019-03-12

Applicant: DAI NIPPON PRINTING CO., LTD. ,  OSAKA UNIVERSITY ,  TERUMO KABUSHIKI KAISHA

Inventor： Taro Nagai ,  Yoshiki Sawa ,  Shigeru Miyagawa ,  Kenji Oyama

IPC: C12M1/00 ,  C12M1/22 ,  C12M1/34 ,  C12M1/38 ,  C12M3/00 ,  C12M3/06 ,  C12M1/02 ,  C12M1/36

Abstract: When detachment of a cell sheet from a culture dish has started at an unintended timing, immediately detach the cell sheet from the culture dish while preventing generation of wrinkles and the like in the cell sheet. A cell culture device captures an image of cells in the culture dish, and controls a cooling mechanism and a shaking mechanism based on the image so as to shake the cells while cooling them.

公开(公告)号：US20220272963A1

公开(公告)日：2022-09-01

申请号：US17664194

申请日：2022-05-19

Applicant: Saraya Co., Ltd. ,  Osaka University

Inventor： Asuka Nogami ,  Motoki Tatsumi ,  Nanase Ishii ,  Mizuyuki Ryu ,  Yoshihiko Hirata ,  Yoshiki Sawa ,  Shigeru Miyagawa ,  Atsuhiro Saito ,  Hirotatsu Ohkawara

IPC: A01N1/02 ,  C12N1/04 ,  C12N5/0775

Abstract: A cell cryopreservation method including adding a 1 volume % of a composition comprising 0.01 wt % to 20 wt % of a sophorose lipid to cells in a cell culture medium just before or up to 6 hours before cryopreserving the cells; and cryopreserving the cell culture medium, wherein the composition improves cell viability after cryopreservation compared to cells that are cryopreserved with a similar composition that does not contain the sophorose lipid.

公开(公告)号：US20200171092A1

公开(公告)日：2020-06-04

申请号：US16620655

申请日：2018-05-08

Applicant: OSAKA UNIVERSITY ,  ROHTO PHARMACEUTICAL CO., LTD.

Inventor： Yoshiki Sawa ,  Shigeru Miyagawa ,  Daisuke Kajita ,  Hayato Kurata ,  Kotoe Tamada ,  Hiroyuki Nishida

IPC: A61K35/28 ,  A61K9/14 ,  A61K38/20 ,  A61K38/18 ,  A61K38/17 ,  A61P9/10

Abstract: The purpose of the present invention is to provide a therapeutic agent for dilated cardiomyopathy disease which produces excellent effects in the treatment of dilated cardiomyopathy (DCM) and provides a given benefit to a large number of patients. The present invention is a therapeutic agent for dilated cardiomyopathy comprising at least one selected from the group consisting of mesenchymal stem cell and microparticles derived from the mesenchymal stem cell. It is preferable that the mesenchymal stem cell is a cell having the ability to contain or secrete microparticles, that the microparticles have an average particle size of 1000 nm or less, and that they are exosomes.

公开(公告)号：US20200045955A1

公开(公告)日：2020-02-13

申请号：US16482236

申请日：2018-01-30

Applicant: Saraya Co., Ltd. ,  Osaka University

Inventor： Asuka Nogami ,  Motoki Tatsumi ,  Nanase Ishii ,  Mizuyuki Ryu ,  Yoshihiko Hirata ,  Yoshiki Sawa ,  Shigeru Miyagawa ,  Atsuhiro Saito ,  Hirotatsu Ohkawara

IPC: A01N1/02 ,  C12N1/04 ,  C12N5/0775

Abstract: This composition can be easily added when cryopreserving cells and improves cell viability. 1 volume % of the composition containing 0.01 wt % to 20 wt % of a sophorose lipid is added just before or up to 6 hours before cryopreservation and cells are stored. This composition can improve cryopreservation cell viability as it reduces freezing damage to cells. With this composition, cells can be stored without using DMSO and blood serum. This is a simple and inexpensive storage method as it does not require fine control of the freezing rate during cell cryopreservation.

公开(公告)号：US20190255116A1

公开(公告)日：2019-08-22

申请号：US16343330

申请日：2017-10-13

Applicant: OSAKA UNIVERSITY ,  ROHTO PHARMACEUTICAL CO., LTD.

Inventor： Yoshiki Sawa ,  Shigeru Miyagawa ,  Daisuke Kajita ,  Kotoe Tamada

IPC: A61K35/28 ,  A61K9/06 ,  A61K38/48 ,  A61K38/36 ,  A61K9/08 ,  A61P9/10

Abstract: The present invention addresses the problem of providing a disease-treating agent which exerts an excellent effect in treating diseases requiring an emergency surgery such as heart failure and which is efficacious at a certain level for a large number of patients. The present invention pertains to a kit for preparing a disease-treating agent, said kit comprising a) a fibrinogen solution, b) a thrombin solution and c) mesenchymal stem cells, in separate forms respectively. It is preferred that c) the mesenchymal stem cells are allogeneic to a subject to be treated. Also, the present invention pertains to a kit for preparing a disease-treating agent, said kit being to be used by, when in use, suspending c) the mesenchymal stem cells in either a) the fibrinogen solution or b) the thrombin solution, and then spraying the cell suspension thus obtained directly to a disease site substantially simultaneously with either b) the thrombin solution or a) the fibrinogen solution that is not used in the suspending step.

公开(公告)号：US20180042220A1

公开(公告)日：2018-02-15

申请号：US15784460

申请日：2017-10-16

Applicant: TERUMO KABUSHIKI KAISHA ,  Osaka University

Inventor： Shigeru MIYAGAWA ,  Yoshiki Sawa ,  Hirotatsu Ohkawara ,  Satsuki Fukushima ,  Atsuhiro Saito ,  Hiroko Iseoka

IPC: A01N1/02

CPC classification number: A01N1/0268 ,  A01N1/021 ,  C12N1/04

Abstract: Methods are disclosed of freezing, cryopreserving, and transferring a frozen sheet-shaped cell culture. The method of freezing includes (1) a step of immersing in a cryopreservation solution a sheet-shaped cell culture supported by a mesh-shaped support body; (2) a step of removing the cryopreservation solution adhered to the sheet-shaped cell culture, while keeping the sheet-shaped cell culture supported by the mesh-shaped support body; (3) a step of enclosing the sheet-shaped cell culture in a cold-resistant film, an upper surface and a lower surface of the sheet-shaped cell culture being covered by the mesh-shaped support body; and (4) a step of freezing the sheet-shaped cell culture.