Method for amplifying genomic DNA
    11.
    发明申请
    Method for amplifying genomic DNA 失效
    扩增基因组DNA的方法

    公开(公告)号:US20090311753A1

    公开(公告)日:2009-12-17

    申请号:US12214044

    申请日:2008-06-16

    IPC分类号: C12P19/34

    摘要: A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.

    摘要翻译: 提供了扩增基因组DNA的方法。 该方法包括以下步骤:(1)在9至12的pH和45至80℃的温度下培养含细胞的琼脂糖溶液以产生基因组DNA分散的琼脂糖溶液,其中0.002-1拷贝/ 5 单链基因组DNA的微升分散; (2)固定基因组DNA分散的琼脂糖溶液以产生基因组DNA分散的琼脂糖凝胶并中和凝胶的pH; 和(3)将具有链置换活性的DNA聚合酶,引物和dNTP加入到基因组DNA分散的琼脂糖凝胶中,并在0至60℃的温度下孵育该凝胶以扩增基因组DNA。

    APPARATUS AND METHOD FOR PRE-CHARGING IN CHARGING/DISCHARGING EQUIPMENT FOR AN ENERGY-STORAGE DEVICE
    12.
    发明申请
    APPARATUS AND METHOD FOR PRE-CHARGING IN CHARGING/DISCHARGING EQUIPMENT FOR AN ENERGY-STORAGE DEVICE 有权
    用于储能装置的充电/放电设备中预充电的装置和方法

    公开(公告)号:US20090256526A1

    公开(公告)日:2009-10-15

    申请号:US12100582

    申请日:2008-04-10

    申请人: Takashi Imai

    发明人: Takashi Imai

    IPC分类号: H02J7/04

    CPC分类号: H02H9/001

    摘要: An apparatus includes a switching circuit operable with an arrangement configured to effectuate a charging or discharging of an energy-storage device to a circuit including electrically-parallel condenser. The switching circuit includes a main switch electrically connectable to the condenser, and a control circuit electrically connected in parallel with the main switch, and electrically connectable to the condenser. The main switch and control circuit are each configured to independently, switchably pass current from the energy-storage device to the condenser and a power load/source. In this regard, the main switch and control circuit are controllable to pass current from the energy-storage device to the power load, and in a manner whereby current from the energy-storage device to the condenser is diverted from passing through the main switch for a period during which a voltage disparity between the condenser and energy-storage device decreases to within a predetermined range.

    摘要翻译: 一种装置包括切换电路,该开关电路可被配置成实现将能量存储装置充电或放电到包括电并联冷凝器的电路的装置。 开关电路包括可与电容器电连接的主开关,以及与主开关并联电连接并与电容器电连接的控制电路。 主开关和控制电路分别被配置为独立地将电流从能量存储装置传递到冷凝器和电力负载/源。 在这方面,主开关和控制电路是可控制的,以将电流从能量存储装置传递到电力负载,并且以使得从储能装置到冷凝器的电流被转向通过主开关, 电容器和储能装置之间的电压差异减小到预定范围内的期间。

    APPARATUS AND METHOD FOR CHARGING AND DISCHARGING SERIALLY-CONNECTED BATTERIES
    13.
    发明申请
    APPARATUS AND METHOD FOR CHARGING AND DISCHARGING SERIALLY-CONNECTED BATTERIES 有权
    充电和放电串联电池的装置和方法

    公开(公告)号:US20090102421A1

    公开(公告)日:2009-04-23

    申请号:US11876797

    申请日:2007-10-23

    申请人: Takashi Imai

    发明人: Takashi Imai

    IPC分类号: H02J7/00

    CPC分类号: H02J7/0014 H02J7/0026

    摘要: An apparatus is provided that includes a plurality of serially-connected bypass circuits, a measurement/control element and a plurality of safety circuits. The bypass circuits are electrically connectable in parallel with a plurality of serially-connected batteries, each bypass circuit being connectable in parallel with a respective battery. The measurement/control element is electrically connected to the bypass circuits, and electrically connectable to the batteries; and the safety circuits are electrically connected to the measurement/control element, and electrically connectable between the measurement/control element and the batteries. The measurement/control element is configured to measure voltages across the respective batteries and, based on the voltages, selectively operate the bypass circuits to divert electrical current to or from the batteries through the respective bypass circuits. And each safety circuit is configured to limit current flow to the measurement/control element.

    摘要翻译: 提供一种装置,其包括多个串联连接的旁路电路,测量/控制元件和多个安全电路。 旁路电路可与多个串联连接的电池并联电连接,每个旁路电路可与相应电池并联连接。 测量/控制元件电连接到旁路电路,并且可电连接到电池; 并且安全电路电连接到测量/控制元件,并且可以在测量/控制元件和电池之间电连接。 测量/控制元件被配置为测量各个电池两端的电压,并且基于电压,选择性地操作旁路电路以通过相应的旁路电路将电流转移到电池或从电池转移电流。 并且每个安全电路被配置为限制流向测量/控制元件的电流。

    Aqueous ink, ink set, and image-forming method
    14.
    发明授权
    Aqueous ink, ink set, and image-forming method 有权
    水性油墨,油墨组合和成像方法

    公开(公告)号:US07402200B2

    公开(公告)日:2008-07-22

    申请号:US11313670

    申请日:2005-12-22

    IPC分类号: C09D11/02

    CPC分类号: C09D11/40 C09D11/54

    摘要: The present invention aims to provide an ink which has a sufficiently large area factor even in a small ink droplet quantity and can obtain images having a high image density, and further has a superior storage stability. According to the present invention, a cyan ink is provided which is used in an ink set having four kinds of aqueous inks consisting of a cyan ink, a magenta ink, a yellow ink and a black ink in which each ink comprises at least water, a water-insoluble coloring material, and a plurality of water-soluble organic solvents having a good medium or good mediums for the water-insoluble coloring material and a poor medium or poor mediums for the water-insoluble coloring material, wherein, the ratio B1/A1 is from 0.5 or more to 3.0 or less, where A1 denotes the total content (mass %) of the good medium based on the total mass of the cyan ink and B1 denotes the total content (mass %) of the poor medium based on the total mass of the cyan ink, and a water-soluble organic solvent which shows the maximum Ka value among the respective Ka values of the plurality of water-soluble organic solvents as determined by the Bristow method is the poor medium.

    摘要翻译: 本发明的目的在于提供即使在小的墨滴量中也具有足够大的面积因数并且可以获得具有高图像密度的图像的墨水,并且还具有优良的储存稳定性。 根据本发明,提供一种青色墨水,其用于具有四种由青色墨水,品红色墨水,黄色墨水和黑色墨水组成的水性墨水的墨水组合,其中每种墨水至少包含水, 水不溶性着色材料和多种水溶性着色材料介质或介质良好的水溶性有机溶剂和水不溶性着色材料的不良介质或不良介质,其中B比 1 1 <1 为0.5以上至3.0以下,其中,A 1表示良好介质的总含量(质量%) 基于青色墨水的总质量,B&lt; 1&lt; 1&gt;表示基于青色墨水的总质量的不良介质的总含量(质量%)和表示 通过Bristow法测定的多种水溶性有机溶剂的各Ka值中的最大Ka值是不良介质。

    Dna Oligomer, Genetic Marker and Dna Oligomer Set for Prediction of Onset of Side-Effect from Radiation Therapy, and Method for Predicting Onset of Side-Effect
    15.
    发明申请
    Dna Oligomer, Genetic Marker and Dna Oligomer Set for Prediction of Onset of Side-Effect from Radiation Therapy, and Method for Predicting Onset of Side-Effect 审中-公开
    Dna寡聚体,遗传标记和Dna寡聚体预测放射治疗副作用的预测及预测副作用发生的方法

    公开(公告)号:US20070264648A1

    公开(公告)日:2007-11-15

    申请号:US11660658

    申请日:2005-09-22

    IPC分类号: C12Q1/68 C07H21/00

    摘要: There are provided a DNA oligomer, a genetic marker, and a DNA oligomer set (PCR primer set) and a DNA oligomer (extension primer) for predicting a possibility of onset of a side-effect from radiation therapy for cancer by determining whether a specific base in a DNA sequence is a risk allele or a non-risk allele, and a method for predicting onset of a side-effect from radiation therapy. The DNA oligomer for a prediction of onset of a side-effect from radiation therapy has a DNA sequence of at least 10-241 contiguous bases with a 121st base from a sequence of any one of SEQ ID NOs: 1-173 in the Sequence Listing.

    摘要翻译: 提供DNA寡聚体,遗传标记和DNA寡聚体(PCR引物组)和DNA寡聚物(延伸引物),用于通过确定是否具体的特异性DNA来预测癌症放射治疗副作用的可能性 DNA序列中的碱基是风险等位基因或非风险等位基因,以及预测放射治疗副作用发生的方法。 用于预测放射治疗副作用发作的DNA寡聚体具有至少10-241个连续碱基的DNA序列,其中序列表中SEQ ID NO:1-173中任一个的序列具有121个碱基 。

    Sheet feeding apparatus, image reading apparatus, and image forming apparatus
    16.
    发明授权
    Sheet feeding apparatus, image reading apparatus, and image forming apparatus 有权
    片材进给装置,图像读取装置和图像形成装置

    公开(公告)号:US07197273B2

    公开(公告)日:2007-03-27

    申请号:US10918989

    申请日:2004-08-16

    IPC分类号: G03G15/00

    CPC分类号: G03G15/6567 G03G15/6564

    摘要: In a sheet feeding apparatus, a contact-type sensor for detecting sheet-like documents stored in document tray is separated into upper document detector and a lower document detector arranged at upper and lower sides of the document tray, respectively. The sheet-like documents are detected separately with two actuators of the corresponding detectors. Accordingly, the requirement of a prior art for having a contact of the sensor increased in length in order to detect the sheets from a maximum to zero in the sheet storing unit can be eliminated.

    摘要翻译: 在片材进给装置中,用于检测存储在文件托盘中的片状文件的接触型传感器分别分别设置在文件托盘的上侧和下侧的上部原稿检测器和下部原稿检测器。 使用相应检测器的两个致动器单独检测片状文档。 因此,可以消除用于使传感器的接触长度增加以便在片材存储单元中从最大到零检测片材的现有技术的要求。