摘要:
A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
摘要:
An apparatus includes a switching circuit operable with an arrangement configured to effectuate a charging or discharging of an energy-storage device to a circuit including electrically-parallel condenser. The switching circuit includes a main switch electrically connectable to the condenser, and a control circuit electrically connected in parallel with the main switch, and electrically connectable to the condenser. The main switch and control circuit are each configured to independently, switchably pass current from the energy-storage device to the condenser and a power load/source. In this regard, the main switch and control circuit are controllable to pass current from the energy-storage device to the power load, and in a manner whereby current from the energy-storage device to the condenser is diverted from passing through the main switch for a period during which a voltage disparity between the condenser and energy-storage device decreases to within a predetermined range.
摘要:
An apparatus is provided that includes a plurality of serially-connected bypass circuits, a measurement/control element and a plurality of safety circuits. The bypass circuits are electrically connectable in parallel with a plurality of serially-connected batteries, each bypass circuit being connectable in parallel with a respective battery. The measurement/control element is electrically connected to the bypass circuits, and electrically connectable to the batteries; and the safety circuits are electrically connected to the measurement/control element, and electrically connectable between the measurement/control element and the batteries. The measurement/control element is configured to measure voltages across the respective batteries and, based on the voltages, selectively operate the bypass circuits to divert electrical current to or from the batteries through the respective bypass circuits. And each safety circuit is configured to limit current flow to the measurement/control element.
摘要:
The present invention aims to provide an ink which has a sufficiently large area factor even in a small ink droplet quantity and can obtain images having a high image density, and further has a superior storage stability. According to the present invention, a cyan ink is provided which is used in an ink set having four kinds of aqueous inks consisting of a cyan ink, a magenta ink, a yellow ink and a black ink in which each ink comprises at least water, a water-insoluble coloring material, and a plurality of water-soluble organic solvents having a good medium or good mediums for the water-insoluble coloring material and a poor medium or poor mediums for the water-insoluble coloring material, wherein, the ratio B1/A1 is from 0.5 or more to 3.0 or less, where A1 denotes the total content (mass %) of the good medium based on the total mass of the cyan ink and B1 denotes the total content (mass %) of the poor medium based on the total mass of the cyan ink, and a water-soluble organic solvent which shows the maximum Ka value among the respective Ka values of the plurality of water-soluble organic solvents as determined by the Bristow method is the poor medium.
摘要:
There are provided a DNA oligomer, a genetic marker, and a DNA oligomer set (PCR primer set) and a DNA oligomer (extension primer) for predicting a possibility of onset of a side-effect from radiation therapy for cancer by determining whether a specific base in a DNA sequence is a risk allele or a non-risk allele, and a method for predicting onset of a side-effect from radiation therapy. The DNA oligomer for a prediction of onset of a side-effect from radiation therapy has a DNA sequence of at least 10-241 contiguous bases with a 121st base from a sequence of any one of SEQ ID NOs: 1-173 in the Sequence Listing.
摘要翻译:提供DNA寡聚体,遗传标记和DNA寡聚体(PCR引物组)和DNA寡聚物(延伸引物),用于通过确定是否具体的特异性DNA来预测癌症放射治疗副作用的可能性 DNA序列中的碱基是风险等位基因或非风险等位基因,以及预测放射治疗副作用发生的方法。 用于预测放射治疗副作用发作的DNA寡聚体具有至少10-241个连续碱基的DNA序列,其中序列表中SEQ ID NO:1-173中任一个的序列具有121个碱基 。
摘要:
In a sheet feeding apparatus, a contact-type sensor for detecting sheet-like documents stored in document tray is separated into upper document detector and a lower document detector arranged at upper and lower sides of the document tray, respectively. The sheet-like documents are detected separately with two actuators of the corresponding detectors. Accordingly, the requirement of a prior art for having a contact of the sensor increased in length in order to detect the sheets from a maximum to zero in the sheet storing unit can be eliminated.
摘要:
Provided are a toner for developing an electrostatic image comprising a crystalline resin having an ester bond and at least one of a sulfide bond or a disulfide bond in the main-chain, an electrostatic image developer and an image-forming process by using the same, a method of producing the toner for developing electrostatic image, and a resin particle dispersion using the same.
摘要:
A reaction solution that is used for image recording with an ink containing a coloring material in a dissolved or dispersed state to destabilize the dissolved or dispersed state of the coloring material in the ink in contact with the ink to provide high quality and abrasion-resistant images, wherein the reaction solution contains at least a polyvalent metal ion and a nonionic polymer, the Ka value according to the Bristow method is from 1.3 mL·m−2·msec−1/2 to 3.0 mL·m−2·msec−1/2 inclusive, and the viscosity is from 20 mPa·s to 150 mPa·s inclusive.
摘要:
The toner for electrostatic charged image development of the present invention is characterized in that it contains at least a binder resin, a mold releasing agent and magnetic metal particles, and the solubility of the magnetic metal particles in a 1 mol/l aqueous HNO3 solution at 50° C. is 500 mg/g·l or less.
摘要翻译:本发明的静电电荷显影用调色剂的特征在于,其至少含有粘合剂树脂,脱模剂和磁性金属颗粒,以及磁性金属颗粒在1mol / l HNO水溶液中的溶解度 > 3℃溶液在500℃下为500mg / g以下。
摘要:
An electrophotographic toner contains a colorant and a binder resin which includes and a crystalline polyester resin as a main component. An ester density M of the crystalline polyester resin, as defined in the following Formula 1, is from 0.01 to 0.12: M=K/A Formula 1 wherein M denotes an ester density, K denotes a number of ester groups, and A denotes a number of atoms which constitute the high molecular chain of a polymer.