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26 条记录 (耗时 0.025 秒)

    Hydroxynitrile lyase
    11.
    发明授权
    Hydroxynitrile lyase 失效

    公开(公告)号：US08383389B2

    公开(公告)日：2013-02-26

    申请号：US13210646

    申请日：2011-08-16

    申请人： Yasuhisa Asano ,  Takanori Akiyama ,  Fujio Yu ,  Eiji Sato

    发明人： Yasuhisa Asano ,  Takanori Akiyama ,  Fujio Yu ,  Eiji Sato

    IPC分类号： C12N9/00 ,  C12N9/88 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/88 ,  C12P7/42 ,  C12P13/004

    摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

    HALOHYDRIN EPOXIDASE
    12.
    发明申请
    HALOHYDRIN EPOXIDASE 失效
    盐酸环氧化酶

    公开(公告)号：US20100136617A1

    公开(公告)日：2010-06-03

    申请号：US12529657

    申请日：2008-03-07

    申请人： Takanori Akiyama ,  Fujio Yu ,  Fumiaki Watanabe ,  Yuya Takikawa ,  Eiji Sato

    发明人： Takanori Akiyama ,  Fujio Yu ,  Fumiaki Watanabe ,  Yuya Takikawa ,  Eiji Sato

    IPC分类号： C12P21/06 ,  C12N9/88 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12P17/02 ,  C12P13/00

    CPC分类号： C12P13/004 ,  C12N9/0071

    摘要： The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.

    摘要翻译： 本发明提供工业上有用的改进的卤代醇环氧酶，其制备方法，以及使用其制备表卤代醇或4-卤代-3-羟基丁腈的方法。 本发明的改进的卤代醇环氧酶由将特定氨基酸取代突变引入包含预定氨基酸序列I和II的野生型卤代醇环氧酶的氨基酸序列中的氨基酸序列组成。

    Process for producing vinylated nucleic acid
    13.
    发明申请
    Process for producing vinylated nucleic acid 审中-公开
    生产乙烯基化核酸的方法

    公开(公告)号：US20060252086A1

    公开(公告)日：2006-11-09

    申请号：US11438463

    申请日：2006-05-23

    申请人： Toshitaka Uragaki ,  Fumiaki Watanabe ,  Chiaki Nagahama ,  Fujio Yu ,  Katsuaki Kikuchi ,  Akira Yoshioka

    发明人： Toshitaka Uragaki ,  Fumiaki Watanabe ,  Chiaki Nagahama ,  Fujio Yu ,  Katsuaki Kikuchi ,  Akira Yoshioka

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C07H21/00

    摘要： This invention provides a process for producing a vinylated nucleic acid in an efficient and cost-effective manner through the implementation of vinylation of an amino-containing nucleic acid with a vinylating agent. This invention also provides a process for producing a vinylated nucleic acid in an efficient and cost-effective manner by a method in which PCR is carried out in the presence of an amino-modified nucleotide and dNTP and a vinyl group is introduced into the resulting PCR amplification product or by a method in which PCR is carried out in the presence of a vinyl-modified nucleotide and cNTP.

    摘要翻译： 本发明提供了一种通过使用乙烯化剂实施含氨基核酸的乙烯化作用，以有效且成本有效的方式生产乙烯基化核酸的方法。 本发明还提供了通过在氨基修饰的核苷酸和dNTP的存在下进行PCR并将乙烯基引入到所得PCR中的方法，以有效和成本有效的方式制备乙烯基化核酸的方法 扩增产物或通过在乙烯基修饰的核苷酸和cNTP存在下进行PCR的方法。

    Circular plasmids
    14.
    发明授权
    Circular plasmids 失效
    圆形质粒

    公开(公告)号：US5705386A

    公开(公告)日：1998-01-06

    申请号：US678650

    申请日：1996-07-11

    申请人： Wataru Mizunashi ,  Fujio Yu

    发明人： Wataru Mizunashi ,  Fujio Yu

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N15/74 ,  C12R1/01 ,  C12N15/63

    CPC分类号： C12N15/74

    摘要： The specification relates to circular plasmids derived from microorganisms of the genus Rhodococcus, which have sizes of about 3.6 kb and restriction site numbers of BamHI:1, BglII:1, ClaI:1, PstI:1, PvuII:2 and SacI:1. The circular plasmids of the present invention are useful as vectors in industrially useful host-vector systems.

    摘要翻译： 本说明书涉及衍生自红球菌属微生物的圆形质粒，其具有约3.6kb的大小和BamHI：1，BglII：1，ClaI：1，PstI：1，PvuII：2和SacI：1的限制性位点编号。 本发明的环状质粒可用作工业上有用的宿主 - 载体系统中的载体。

    HYDROXYNITRILE LYASE
    17.
    发明申请
    HYDROXYNITRILE LYASE 失效
    羟肟酸钠

    公开(公告)号：US20120040417A1

    公开(公告)日：2012-02-16

    申请号：US13210646

    申请日：2011-08-16

    申请人： Yasuhisa ASANO ,  Takanori Akiyama ,  Fujio Yu ,  Eiji Sato

    发明人： Yasuhisa ASANO ,  Takanori Akiyama ,  Fujio Yu ,  Eiji Sato

    IPC分类号： C12P13/00 ,  C12N9/88 ,  C12N5/10 ,  C12N15/60 ,  C12N1/15 ,  C12N1/19 ,  C12N15/63 ,  C12P7/42 ,  C12N1/21

    CPC分类号： C12N9/88 ,  C12P7/42 ,  C12P13/004

    摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

    摘要翻译： 改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性 引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。

    Nitrilase gene
    20.
    发明授权
    Nitrilase gene 失效
    腈水解酶基因

    公开(公告)号：US5872000A

    公开(公告)日：1999-02-16

    申请号：US690493

    申请日：1996-07-31

    申请人： Fujio Yu

    发明人： Fujio Yu

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/78 ,  C12N15/00 ,  C12N15/55 ,  C12R1/01 ,  C12R1/19 ,  C12P19/34

    CPC分类号： C12N9/78

    摘要： The invention relates to a DNA coding for a polypeptide having nitrilase activity with an amino acid sequence as shown in SEQ ID NO:1. As compared with conventional processes, the hydrolysis of nitriles by a nitrilase gene cloned by genetic recombination is expected to drastically improve the catalytic ability of microorganisms because they can be engineered to contain multiple copies of the same gene.

    摘要翻译： 本发明涉及编码具有氨基酸序列的具有腈水解酶活性的多肽的DNA，如SEQ ID NO：1所示。 与常规方法相比，通过基因重组克隆的腈水解酶基因对腈的水解预期将显着提高微生物的催化能力，因为它们可被工程化以含有相同基因的多个拷贝。