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105 条记录 (耗时 0.034 秒)

    Method for the synthesis of chiral cyanohydrins via a hydroxynitrile lyase from brassicaceae
    2.
    发明授权
    Method for the synthesis of chiral cyanohydrins via a hydroxynitrile lyase from brassicaceae 有权
    通过芸苔科的羟基腈裂解酶合成手性氰醇的方法

    公开(公告)号：US08940516B2

    公开(公告)日：2015-01-27

    申请号：US13680660

    申请日：2012-11-19

    申请人： Evocatal GmbH

    发明人： Thorsten Eggert ,  Jennifer Andexer

    IPC分类号： C12P13/00 ,  C12P7/26 ,  C12N9/88 ,  C12P21/00

    CPC分类号： C12N9/88 ,  C12P13/004

    摘要： The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.

    摘要翻译： 本发明涉及一种多肽，其可从十字花科科中分离并且至少具有羟基腈裂解酶（HNL）的活性。 本发明的羟基腈裂解酶是来自十字花科科的第一种HNL。 这种酶或其基因被分离的植物（拟南芥）也被描述为非氰化的。 到目前为止描述的所有含有HNL的植物都是氰基植物，因此迄今为止已经假设只有氰基植物含有羟基腈裂解酶。 令人惊奇的是，发现本发明的多肽（AtHNL）是（R）选择性的。 氨基酸序列为AtHNL亚基的理论分子量为29.2 kDa。 约30kDa的蛋白质的计算分子量可以通过SDS凝胶电泳确认。

    Halohydrin epoxidase
    3.
    发明授权
    Halohydrin epoxidase 失效
    卤代醇环氧酶

    公开(公告)号：US08637272B2

    公开(公告)日：2014-01-28

    申请号：US12529657

    申请日：2008-03-07

    申请人： Takanori Akiyama ,  Yu Fujio ,  Fumiaki Watanabe ,  Yuya Takikawa ,  Eiji Sato

    发明人： Takanori Akiyama ,  Yu Fujio ,  Fumiaki Watanabe ,  Yuya Takikawa ,  Eiji Sato

    IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/88 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P13/004 ,  C12N9/0071

    摘要： The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.

    摘要翻译： 本发明提供工业上有用的改进的卤代醇环氧酶，其制备方法，以及使用其制备表卤代醇或4-卤代-3-羟基丁腈的方法。 本发明的改进的卤代醇环氧酶由将特定氨基酸取代突变引入包含预定氨基酸序列I和II的野生型卤代醇环氧酶的氨基酸序列中的氨基酸序列组成。

    Chemoenzymatic methods for the synthesis of statins and statin intermediates
    4.
    发明授权
    Chemoenzymatic methods for the synthesis of statins and statin intermediates 有权
    用于合成他汀类药物和他汀类药物中间体的化疗酶法

    公开(公告)号：US08148324B2

    公开(公告)日：2012-04-03

    申请号：US12032337

    申请日：2008-02-15

    申请人： William Greenberg ,  Mark J. Burk ,  Alexander Varvak ,  Kelvin Wong

    发明人： William Greenberg ,  Mark J. Burk ,  Alexander Varvak ,  Kelvin Wong

    IPC分类号： C07K14/00 ,  C12P21/00

    CPC分类号： C12P13/004 ,  C12N9/88 ,  C12P7/42 ,  C12P17/02 ,  C12P17/06 ,  C12P17/10 ,  C12P17/12

    摘要： The invention provides aldolases, nucleic acids encoding them and methods for making and using them, including chemoenzymatic processes for making β,δ-dihydroxyheptanoic acid side chains and compositions comprising these side chains, e.g., [R—(R*,R*)]-2-(4-fluorophenyl)-b,d-dihydroxy-5-(1-methylethyl)-3-phenyl-4-(phenylamino)-carbonyl]-1H-pyrrole-1-heptanoic acid (atorvastatin, LIPITOR™), rosuvastatin (CRESTOR™), fluvastatin (LESCOL™), related compounds and their intermediates.

    摘要翻译： 本发明提供醛缩酶，编码它们的核酸和制备和使用它们的方法，包括制备和/或制备δ-二羟基庚酸侧链的化学方法和包含这些侧链的组合物，例如[R-（R *，R *） ] -2-（4-氟苯基）-b，d-二羟基-5-（1-甲基乙基）-3-苯基-4-（苯基氨基） - 羰基] -1H-吡咯-1-庚酸（阿托伐他汀，LIPITOR TM ），瑞舒伐他汀（CRESTOR TM），氟伐他汀（LESCOL TM），相关化合物及其中间体。

    R-Hydroxynitrile Lyases Having Improved Substrate Acceptance and the Use Thereof
    6.
    发明申请
    R-Hydroxynitrile Lyases Having Improved Substrate Acceptance and the Use Thereof 审中-公开
    具有改善底物接受性的R-羟基腈裂解物及其用途

    公开(公告)号：US20100041110A1

    公开(公告)日：2010-02-18

    申请号：US11795685

    申请日：2005-12-28

    申请人： Roland Weis ,  Anton Glieder ,  Karl Gruber ,  Wolfgang Skrang ,  Oliver Maurer ,  Richard Gaisberger

    发明人： Roland Weis ,  Anton Glieder ,  Karl Gruber ,  Wolfgang Skrang ,  Oliver Maurer ,  Richard Gaisberger

    IPC分类号： C12P13/00 ,  C12N9/88

    CPC分类号： C12P13/004 ,  C12N9/88

    摘要： R-hydroxynitrile lyases having an improved substrate acceptance, increased activity and increased selectivity, in which there is replacement in the amino acid sequence of R-hydroxynitrile lyases from the Rosaceae family either a) of the amino acid residue which corresponds to position 360 of the mature PaHNL5 protein by another apolar amino acid or a neutral amino acid and/or b) of the amino acid residue which corresponds to position 225 of the mature PaHNL5 protein by another polar amino acid, it also being possible where appropriate for 1 to 20 further residues in the active center or in the hydrophobic channel leading to the active center to be replaced.

    摘要翻译： 具有改善的底物接受性，增加的活性和增加的选择性的R-羟基腈裂解酶，其中在来自蔷薇科家族的R-羟基腈裂解酶的氨基酸序列中置换a）的氨基酸残基，其对应于 通过另一个非极性氨基酸或中性氨基酸的成熟PaHNL5蛋白和/或b）与另一种极性氨基酸对应的成熟PaHNL5蛋白的位置225的氨基酸残基，在适当的情况下还可以进一步加入1至20 活性中心或疏水通道中的残留物，导致活性中心被更换。

    NOVEL HIGH-ACTIVITY MODIFIED S-HYDROXYNITRILE LYASE
    7.
    发明申请
    NOVEL HIGH-ACTIVITY MODIFIED S-HYDROXYNITRILE LYASE 审中-公开
    新的高活性修饰的S-羟基蛋白LYASE

    公开(公告)号：US20090239273A1

    公开(公告)日：2009-09-24

    申请号：US12094330

    申请日：2006-09-29

    申请人： Hisashi Semba ,  Eita Ichige

    发明人： Hisashi Semba ,  Eita Ichige

    IPC分类号： C12P13/00 ,  C12N9/88 ,  C07H21/04

    CPC分类号： C12N9/88 ,  C12P13/004

    摘要： This invention relates to a novel high-activity modified S-hydroxynitrile lyase (SHNL). More particularly, this invention relates to novel high-activity modified SHNL that is obtained by substituting amino acids at given sites (14, 44, 66, 94, 103, 118, 122, 125, 127, 129, 147, 148, 152, 212, and 216) or inserting amino acid at a given site (between amino acids 128 and 129) of the amino acid sequence (SEQ ID NO: 2) of wild-type SHNL.

    摘要翻译： 本发明涉及一种新的高活性修饰的S-羟基腈裂解酶（SHNL）。 更具体地说，本发明涉及通过在给定位点（14,44,66,94,103,118,122,125,127,129,147,148,152,154,144,144,134,144,134,144,134,132,134,144,134,144,134,132,144,134,132,134,134,144,134,144,134,132,144,134,142,144,144,13 212和216）或在野生型SHNL的氨基酸序列（SEQ ID NO：2）的给定位点（第128和129位氨基酸之间）插入氨基酸。

    Process for preparing heterocyclic (R)- and (S)-cyanohydrins
    9.
    发明授权
    Process for preparing heterocyclic (R)- and (S)-cyanohydrins 失效
    制备杂环（R） - 和（S） - 氰基醇的方法

    公开(公告)号：US07052885B2

    公开(公告)日：2006-05-30

    申请号：US10325922

    申请日：2002-12-23

    申请人： Peter Poechlauer ,  Wolfgang Skranc ,  Herbert Mayrhofer ,  Irma Wirth ,  Rudolf Neuhofer ,  Herfried Griengl ,  Martin Fechter

    发明人： Peter Poechlauer ,  Wolfgang Skranc ,  Herbert Mayrhofer ,  Irma Wirth ,  Rudolf Neuhofer ,  Herfried Griengl ,  Martin Fechter

    IPC分类号： C12P17/00 ,  C12P17/02 ,  C12P17/04 ,  C12P17/06 ,  C12P17/18

    CPC分类号： C12P13/004 ,  C12P17/00 ,  C12P17/04

    摘要： A process for preparing enantiomer-enriched heterocyclic (R)- and (S)-cyanohydrins of the formula (I), where R1, R2, R3, R4 independently of one another are H, an unsubstituted or substituted C1–C24-alkyl, alkenyl or alkynyl radical, where one or more carbon atoms in the chain can be replaced by an oxygen atom, a nitrogen atom, a sulfur atom, an SO or an SO2 group, an unsubstituted or substituted aryl or heteroaryl radical or heterocyclic radical or halogen, hydroxyl, NR5R6, acetyl, oxo, C1–C6-carbalkoxy, C1–C6-carbalkoxyamino, COOR7, cyano, amide, benzoylamino or NO2,R5 and R6 are H, C1–C6-alkyl radical, phenyl radical, benzyl radical or COOR7, or together form a C2–C8-alkylene or heteroalkylene radical,R7 is H or C1–C6-alkyl,n is 0, 1, 2 or 3,X, Y and Z can be an unsubstituted or substituted carbon atom or a radical selected from the group consisting of N, O, S or NR5R6, where R5 and R6 are as defined above, SO or SO2, and at least one of the radicals X, Y and Z is not a carbon atom, where the compounds of the formula (I) can have one or more double bonds in the ring, with the proviso that in a 5-membered ring the double bond is not conjugated with the —C(OH)CN-group, and/or can be anellated and/or bridged,by reacting a ketone of the formula (II), where R1, R2, R3, R4, X, Y, Z and n have the meanings above, with an (R)- or (S)-hydroxynitrile lyase in an organic, aqueous or two-phase system, or in emulsion, in the presence of a cyanide group donor.

    摘要翻译： 制备式（I）的对映异构体富集的杂环（R） - 和（S） - 氰基醇的方法，其中R 1，R 2，R 3，R 4彼此独立地是H，未取代或取代的C 1 - 链烯基或炔基，其中链中的一个或多个碳原子可以被氧原子，氮原子，硫原子，SO或 SO 2 2基团，未取代或取代的芳基或杂芳基或杂环基或卤素，羟基，NR 5 R 6，乙酰基，氧代C 1 -C 6 - 亚烷基氧基，C 1 -C 6 - 烷氧基氨基，COOR 7，氰基，酰胺，苯甲酰氨基或NO 2，R 5和R 6是H C 1 -C 6 - 烷基，苯基，苄基或COOR 7，或一起形成C 1 -C 6 - 8亚烷基或杂亚烷基，R 7是H或C 1 -C 6 - 烷基，n是0,1,2或3，X，Y 并且Z可以是未取代或取代的碳原子或选自它的基团 由N，O，S或NR5R6组成的基团，其中R 5和R 6如上定义，SO或SO 2，并且基团X，Y和Z中的至少一个不是碳 原子，其中式（I）的化合物可以在环中具有一个或多个双键，条件是在5元环中双键不与-C（OH）CN-基团共轭，并且 /或可以通过使式（II）的酮（其中R 1，R 2，R 3，R 4，X，Y，Z和n具有上述含义）与（R） - 或（ S） - 羟基腈裂解酶在有机，水相或两相体系中，或在乳液中，在氰化物基团给体存在下。

    R-hydroxynitrillyases havign improved substrate tolerance and the use thereof
    10.
    发明申请
    R-hydroxynitrillyases havign improved substrate tolerance and the use thereof 失效
    R-hydroxynitrillyases有利于改善底物耐受性及其用途

    公开(公告)号：US20060105434A1

    公开(公告)日：2006-05-18

    申请号：US10548271

    申请日：2004-02-24

    申请人： Wolfgang Skranc ,  Anton Glieder ,  Karl Gruber ,  Roland Weis ,  Ruud Luiten

    发明人： Wolfgang Skranc ,  Anton Glieder ,  Karl Gruber ,  Roland Weis ,  Ruud Luiten

    IPC分类号： C12P13/04 ,  C12P13/00 ,  C12N9/88

    CPC分类号： C12P13/004 ,  C12N9/88

    摘要： The invention related to R-hydroxynitrillyases from the family of Rosaceae that are characterized by an improved substrate tolerance and increased stability. In the active center of the R-hydroxynitrillyases either a) an alanine group is substituted by glycine, valine, leucine, isoleucine, or phenylalanine or b) a phenylalanine group is substituted by alanine, glycine valine, leucine or isoleucine, or c) a leucine group is substituted by alanine, glycine, valine, isoleucine or phenylalanine, or d) an isoleucine group is substituted by alanine, glycine, valine, leucine or phenylalanine. The invention also relates to the use of these lyases in the production of enantiomer-pure R- or S-cyanohydrines.

    摘要翻译： 本发明涉及来自蔷薇科家族的R-羟基亚硝酸酯酶，其特征在于改善的底物耐受性和增加的稳定性。 或者a）丙氨酸被甘氨酸，缬氨酸，亮氨酸，异亮氨酸或苯丙氨酸取代，或b）苯丙氨酸被丙氨酸，甘氨酸缬氨酸，亮氨酸或异亮氨酸取代，或c） 亮氨酸被丙氨酸，甘氨酸，缬氨酸，异亮氨酸或苯丙氨酸取代，或d）异亮氨酸被丙氨酸，甘氨酸，缬氨酸，亮氨酸或苯丙氨酸取代。 本发明还涉及这些裂解酶在制备对映异构体纯的R-或S-氰基中的用途。