公开(公告)号：US20200048606A1

公开(公告)日：2020-02-13

申请号：US16455118

申请日：2019-06-27

Applicant: The Regents of the University of California ,  Berkeley Lights, Inc.

Inventor： Alexander Marson ,  Gregory G. Lavieu ,  Annamaria Mocciaro ,  Theodore L. Roth ,  Magali Soumillon ,  Hayley M. Bennett

IPC: C12N5/0783 ,  B01L3/00 ,  C12N15/10

Abstract: Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.

公开(公告)号：US12234461B2

公开(公告)日：2025-02-25

申请号：US18463589

申请日：2023-09-08

Applicant: The Regents of the University of California

Inventor： Theodore Lee Roth ,  Alexander Marson

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90

Abstract: Provided herein are methods and compositions for editing the genome of a cell. In some embodiments, a nucleotide sequence of at least 200 nucleotides in length is inserted into a target region in the genome of a cell.

公开(公告)号：US20250041338A1

公开(公告)日：2025-02-06

申请号：US18710157

申请日：2022-11-17

Applicant: The Regents of the University of California ,  The J. David Gladstone Institutes, a testamentary trust established under the will of J. David

Inventor： Alexander Marson ,  Eric Shifrut ,  Julia Carnevale ,  Alan Ashworth

IPC: A61K35/17 ,  A61K39/00 ,  A61P35/00 ,  C12N5/0783 ,  C12N15/90

Abstract: Provided herein are genetically modified T cells that exhibit increased proliferation compared to wild-type T cells when stimulated, methods of generating such T cells, and methods of using the T cells for the treatment of a disease such as cancer.

公开(公告)号：US20250025576A1

公开(公告)日：2025-01-23

申请号：US18713033

申请日：2022-11-22

Applicant: The Regents of the University of California

Inventor： Ross C. Wilson ,  Dana V. Foss ,  John Hunsinger ,  Daniel Carr ,  Alexander Marson ,  David-Huy Nguyen ,  Srishti U. Sahu

IPC: A61K48/00 ,  C07K16/28 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides a cargo delivery fusion polypeptide comprising an endosomolytic polypeptide and a cell penetrating polypeptide; and compositions comprising the cargo delivery fusion polypeptide. The present disclosure also provides methods of delivering a cargo into a target eukaryotic cell, using a composition comprising an amphiphilic polypeptide.

公开(公告)号：US20240117360A1

公开(公告)日：2024-04-11

申请号：US18463589

申请日：2023-09-08

Applicant: The Regents of the University of California

Inventor： Theodore Lee Roth ,  Alexander Marson

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90

CPC classification number: C12N15/1138 ,  C12N9/22 ,  C12N15/102 ,  C12N15/90 ,  C12N2310/20 ,  C12N2320/53

Abstract: Provided herein are methods and compositions for editing the genome of a cell. In some embodiments, a nucleotide sequence of at least 200 nucleotides in length is inserted into a target region in the genome of a cell.

公开(公告)号：US20240115705A1

公开(公告)日：2024-04-11

申请号：US18274307

申请日：2022-02-04

Applicant: The J. Gladstone Institutes, a testamentary trust established under the will of J. David Gladstone ,  The Regents of the University of California

Inventor： Alexander Marson ,  Murad Mamedov

IPC: A61K39/00 ,  A61K45/06 ,  A61P35/00 ,  C12N15/86 ,  G01N33/50

CPC classification number: A61K39/464429 ,  A61K39/4611 ,  A61K45/06 ,  A61P35/00 ,  C12N15/86 ,  G01N33/5011 ,  C12N2740/15043

Abstract: Described herein are positive and negative regulators of BTN3A, as well as methods for identifying subjects who can benefit from T cell therapies and/or various chemotherapies. The subjects can for example be suffering from immune disorders, cancer and other diseases and conditions.

公开(公告)号：US11814624B2

公开(公告)日：2023-11-14

申请号：US17498531

申请日：2021-10-11

Applicant: The Regents of the University of California

Inventor： Theodore Lee Roth ,  Alexander Marson

IPC: C12N15/113 ,  C12N15/10 ,  C12N15/90 ,  C12N9/22

CPC classification number: C12N15/1138 ,  C12N9/22 ,  C12N15/102 ,  C12N15/90 ,  C12N2310/20 ,  C12N2320/53

Abstract: Provided herein are methods and compositions for editing the genome of a cell. In some embodiments, a nucleotide sequence of at least 200 nucleotides in length is inserted into a target region in the genome of a cell.

公开(公告)号：US11590171B2

公开(公告)日：2023-02-28

申请号：US17725478

申请日：2022-04-20

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Theodore Lee Roth ,  Eric Shifrut ,  Alexander Marson ,  Cristina Puig Saus ,  Antoni Ribas

IPC: A61K35/17 ,  C07K14/725 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90

Abstract: Provided herein are methods and compositions for editing the genome of a human T cell. In some embodiments, a heterologous T cell receptor (TCR)-β chain and a heterologous TCR-α chain are inserted into exon 1 of a TCR subunit constant gene in the genome of the T cell.

公开(公告)号：US20210388362A1

公开(公告)日：2021-12-16

申请号：US17326252

申请日：2021-05-20

Applicant: The Regents of the University of California

Inventor： Theodore Lee Roth ,  Alexander Marson

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90

Abstract: Provided herein are methods and compositions for editing the genome of a cell. In some embodiments, a nucleotide sequence of at least 200 nucleotides in length is inserted into a target region in the genome of a cell.

公开(公告)号：US11033584B2

公开(公告)日：2021-06-15

申请号：US16568116

申请日：2019-09-11

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Theodore Lee Roth ,  Eric Shifrut ,  Alexander Marson ,  Cristina Puig Saus ,  Antoni Ribas

IPC: A61K35/17 ,  C07K14/725 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90

Abstract: Provided herein are methods and compositions for editing the genome of a human T cell. In some embodiments, a heterologous T cell receptor (TCR)-β chain and a heterologous TCR-α chain are inserted into exon 1 of a TCR subunit constant gene in the genome of the T cell.