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    Methods for selection and generation of genome edited T cells
    1.
    发明授权
    Methods for selection and generation of genome edited T cells 有权

    公开(公告)号：US11639495B2

    公开(公告)日：2023-05-02

    申请号：US16455118

    申请日：2019-06-27

    申请人： The Regents of the University of California ,  Berkeley Lights, Inc.

    发明人： Alexander Marson ,  Gregory G. Lavieu ,  Annamaria Mocciaro ,  Theodore L. Roth ,  Magali Soumillon ,  Hayley M. Bennett

    IPC分类号： C12N5/0783 ,  B01L3/00 ,  C12N15/10

    摘要： Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.

    Methods for Selection and Generation of Genome Edited T Cells
    2.
    发明申请
    Methods for Selection and Generation of Genome Edited T Cells 审中-公开

    公开(公告)号：US20200048606A1

    公开(公告)日：2020-02-13

    申请号：US16455118

    申请日：2019-06-27

    申请人： The Regents of the University of California ,  Berkeley Lights, Inc.

    发明人： Alexander Marson ,  Gregory G. Lavieu ,  Annamaria Mocciaro ,  Theodore L. Roth ,  Magali Soumillon ,  Hayley M. Bennett

    IPC分类号： C12N5/0783 ,  B01L3/00 ,  C12N15/10

    摘要： Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.