公开(公告)号：US11327075B2

公开(公告)日：2022-05-10

申请号：US16326687

申请日：2017-08-21

申请人： The Regents of the University of California

发明人： Daniel Takashi Kamei ,  Benjamin Ming Wu ,  David Yuan Pereira ,  Chloe Michelle Wu ,  Matthew Foosing Yee ,  Joshua Anthony Keefe ,  Christina Caroline Pearce ,  Amir Adam Dailamy ,  Vincent K. Wong ,  Nguyen Khoi Le

IPC分类号： G01N33/559 ,  G01N33/558 ,  G01N33/543 ,  G16H30/40 ,  G01N33/487 ,  G01N33/545 ,  G01N33/569 ,  C08J3/075 ,  G01N1/28

摘要： It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.

公开(公告)号：US20200284791A1

公开(公告)日：2020-09-10

申请号：US16751114

申请日：2020-01-23

申请人： The Regents of the University of California

发明人： Daniel Takashi Kamei ,  Yin To Chiu ,  Benjamin Ming Wu ,  Garrett L. Mosley

IPC分类号： G01N33/558 ,  G01N33/543

摘要： Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.

公开(公告)号：US20190250156A1

公开(公告)日：2019-08-15

申请号：US16307125

申请日：2017-06-07

申请人： The Regents of the University of California

发明人： Daniel Takashi Kamei ,  Benjamin Ming Wu ,  Daniel William Bradbury ,  Shin Ting Sherine Frieda Cheung

IPC分类号： G01N33/543

CPC分类号： G01N33/54386 ,  C12Q1/6813 ,  C12Q1/6844 ,  G01N33/53 ,  G01N33/543 ,  G01N33/54306 ,  G01N33/558 ,  C12Q2563/159 ,  C12Q2565/625 ,  C12Q2521/513 ,  C12Q2531/119 ,  C12Q2531/143

摘要： In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.