公开(公告)号：US20090049574A1

公开(公告)日：2009-02-19

申请号：US12173868

申请日：2008-07-16

申请人： Emil M. Orozco, Jr. ,  Zude Weng ,  Wesley B. Bruce ,  Rebecca E. Cahoon ,  Yong Tao

发明人： Emil M. Orozco, Jr. ,  Zude Weng ,  Wesley B. Bruce ,  Rebecca E. Cahoon ,  Yong Tao

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/10 ,  A01H5/00

CPC分类号： C07K14/415 ,  C12N15/8294

摘要： This invention relates to an isolated nucleic acid fragment encoding an auxin transport protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the auxin transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the auxin transport protein in a transformed host cell. The present invention also relates to methods using the auxin transport protein in modulating root development, and in discovering compounds with potential herbicidal activity.

摘要翻译： 本发明涉及编码生长素转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或大部分生长素转运蛋白的嵌合基因，其中嵌合基因的表达导致转化宿主中生长素转运蛋白水平的变化 细胞。 本发明还涉及使用生长素转运蛋白调节根发育的方法，以及发现具有潜在除草活性的化合物。

公开(公告)号：US06399859B1

公开(公告)日：2002-06-04

申请号：US08987367

申请日：1997-12-10

申请人： Scott E. Nichols ,  Kanwarpal S. Dhugga ,  George W. Singletary ,  Court A. Saunders ,  Derrick Witcher ,  Wesley B. Bruce ,  Lynne E. Sims ,  Guihua Lu ,  Gan-Yuan Zhong

发明人： Scott E. Nichols ,  Kanwarpal S. Dhugga ,  George W. Singletary ,  Court A. Saunders ,  Derrick Witcher ,  Wesley B. Bruce ,  Lynne E. Sims ,  Guihua Lu ,  Gan-Yuan Zhong

IPC分类号： C12N1582

CPC分类号： C12N9/0006 ,  A01K2217/05 ,  A23K10/30 ,  C12N15/8242 ,  C12N15/8245 ,  C12N15/8261 ,  C12N15/8286 ,  Y02A40/146 ,  Y02A40/162

摘要： Isolated nucleic acid molecules are provided that encode maize UDPGdH variant UDPGdH, and mutant UDPGdH proteins. These nucleic acid molecules can be used to produce transgenic plants having altered quality or quantity of starch. Also provided are vectors capable of expressing such nucleic acid molecules, host cells containing, such vectors, and polypeptides encoded by such nucleic acids.

摘要翻译： 提供了编码玉米UDPGdH变体UDPGdH和突变型UDPGdH蛋白质的分离的核酸分子。 这些核酸分子可用于产生具有改变的淀粉质量或数量的转基因植物。 还提供了能够表达这种核酸分子的载体，含有此类载体的宿主细胞和由该核酸编码的多肽。

公开(公告)号：US06388066B1

公开(公告)日：2002-05-14

申请号：US09405779

申请日：1999-09-27

申请人： Wesley B. Bruce ,  Sheila E. Maddock

发明人： Wesley B. Bruce ,  Sheila E. Maddock

IPC分类号： C07H2104

CPC分类号： C12N15/8216 ,  C12N15/822 ,  C12N15/8222 ,  C12N15/8227 ,  C12N15/8234

摘要： This invention discloses to compositions and methods for altering the characteristic pattern of expression associated with a promoter. More particularly the constitutive expression pattern associated with the small synthetic promoter, Rsyn7 is modified so that expression of a heterologous nucleotide sequence operably linked to the Rsyn7 promoter is in a tissue localized manner. This modification of the Rsyn7 pattern of expression occurs as a result of the addition of matrix attachment region DNA sequences to the flanks or 5′ and 3′ ends of an expression cassette comprising the Rsyn7 promoter operably linked to a heterologous nucleotide sequence of interest. DNA constructs, transformed plant cells and transformed plants are provided.

摘要翻译： 本发明公开了用于改变与启动子相关的表达特征模式的组合物和方法。 更具体地，与小合成启动子Rsyn7相关的组成型表达模式被修饰，使得可操作地连接到Rsyn7启动子的异源核苷酸序列的表达是组织定位的。 Rsyn7模式表达的这种修饰是由于将基质连接区DNA序列添加到包含可操作地连接到感兴趣的异源核苷酸序列的Rsyn7启动子的表达盒的侧翼或5'和3'末端而进行的。 提供了DNA构建体，转化的植物细胞和转化的植物。

公开(公告)号：US20100306874A1

公开(公告)日：2010-12-02

申请号：US12599515

申请日：2008-05-15

申请人： Wesley B. Bruce ,  Olga N. Danilevskaya ,  Lisa J. Newman

发明人： Wesley B. Bruce ,  Olga N. Danilevskaya ,  Lisa J. Newman

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/63 ,  A01H5/00 ,  C12N9/12

CPC分类号： C12N15/8261 ,  C12N9/1205 ,  Y02A40/146

摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots, and for increasing yield in a plant are provided. The compositions include a ZmPKT sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1 and 2 as well as variants and fragments thereof. Nucleotide sequences encoding the ZmPKT polypeptides are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a ZmPKT sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a ZmPKT sequence of the invention. The level of the ZmPKT polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.

摘要翻译： 提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。 组合物包括ZmPKT序列。 本发明的组合物包含选自SEQ ID NO：1和2的氨基酸序列和核苷酸序列以及其变体和片段。 在DNA构建体中提供了编码ZmPKT多肽的核苷酸序列，用于在感兴趣的植物中表达以提供植物中的ZmPKT序列的水平或植物部分。 所述方法包括向植物或植物部分引入包含本发明的ZmPKT序列的异源多核苷酸。 可以增加或减少ZmPKT多肽的水平。 这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

公开(公告)号：US07256322B2

公开(公告)日：2007-08-14

申请号：US10744572

申请日：2003-12-23

申请人： Keith S. Lowe ,  Rebecca E. Cahoon ,  Christopher J. Scelonge ,  Yumin Tao ,  William J. Gordon-Kamm ,  Wesley B. Bruce ,  Lisa J. Newman

发明人： Keith S. Lowe ,  Rebecca E. Cahoon ,  Christopher J. Scelonge ,  Yumin Tao ,  William J. Gordon-Kamm ,  Wesley B. Bruce ,  Lisa J. Newman

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8207 ,  C07K14/415 ,  C12N15/8201 ,  C12N15/821 ,  C12N15/8241 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。

公开(公告)号：US06555673B1

公开(公告)日：2003-04-29

申请号：US09556163

申请日：2000-04-21

申请人： Benjamin A. Bowen ,  Wesley B. Bruce ,  Guihua Lu ,  Lynne E. Sims ,  Laura A. Tagliani

发明人： Benjamin A. Bowen ,  Wesley B. Bruce ,  Guihua Lu ,  Lynne E. Sims ,  Laura A. Tagliani

IPC分类号： C07H2104

CPC分类号： C12N9/0008 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8226 ,  C12N15/8279

摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a “TATA to start” sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.

摘要翻译： 公开了用于增强植物细胞中基因表达的合成元件。 这些包括具有含有64％或更高GC含量的“TATA起始”序列的启动子和包含若干OCS结合基序和新侧翼序列的合成上游元件。 还公开了上游活化区（UAR），当它们可操作地连接到所述启动子和/或合成上游元件时，可以进一步增加组成型转录活性。 特别地，提供了玉米Ubi-1基因的UAR的核苷酸序列，并且其用于表达盒和含有这些启动子元件的载体。 进一步提供用这些载体转化的细胞和植物。 这些包括在具有35S CaMV启动子的至少一个上游活化区的重组启动子的转录控制下在高水平表达外源性草酸氧化酶基因的转基因向日葵。

公开(公告)号：US5804694A

公开(公告)日：1998-09-08

申请号：US554169

申请日：1995-11-06

申请人： Wesley B. Bruce ,  Elizabeth Hood ,  David J. Peterson ,  James C. Register, III ,  Derrick Witcher ,  John A. Howard

发明人： Wesley B. Bruce ,  Elizabeth Hood ,  David J. Peterson ,  James C. Register, III ,  Derrick Witcher ,  John A. Howard

IPC分类号： C12N9/24 ,  C12N15/55 ,  C12N15/82 ,  C12N15/29 ,  A01H4/00 ,  A01H5/00

CPC分类号： C12Y302/01031 ,  C12N15/8242 ,  C12N15/8257 ,  C12N9/2434

摘要： A method for commercial production of GUS entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the GUS-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of GUS expression and that can be used as a site of integration for high level expression of other genes of interest.

摘要翻译： 用于商业生产GUS的方法需要以天然构象在植物中以表达水平异源表达蛋白质，使得抗生物素蛋白占总提取蛋白质的至少0.1％。 整合基因座的遗传图可以鉴定表达GUS的植物。 显示植物染色体上的遗传基因座，其支持高水平的GUS表达，并且可以用作整合用于其他感兴趣的基因的高水平表达的位点。

公开(公告)号：US20100218273A1

公开(公告)日：2010-08-26

申请号：US12602711

申请日：2008-06-09

申请人： Wesley B. Bruce

发明人： Wesley B. Bruce

IPC分类号： C12N15/82 ,  C12N15/29 ,  C12N15/63 ,  A01H5/00 ,  C07K14/415

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots, and for increasing yield in a plant are provided. The compositions include a SILKY1 sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1 and 2 as well as variants and fragments thereof. Nucleotide sequences encoding the SILKY1 sequences are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a SILKY1 sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a SILKY1 sequence of the invention. The level of the SILKY1 polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.

摘要翻译： 提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。 组合物包括SILKY1序列。 本发明的组合物包含选自SEQ ID NO：1和2的氨基酸序列和核苷酸序列以及其变体和片段。 提供编码SILKY1序列的核苷酸序列用于在感兴趣的植物中表达的DNA构建体用于调节植物中的SILKY1序列的水平或植物部分。 所述方法包括向植物或植物部分引入包含本发明的SILKY1序列的异源多核苷酸。 SILKY1多肽的水平可以增加或减少。 这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

公开(公告)号：US07179963B2

公开(公告)日：2007-02-20

申请号：US10430523

申请日：2003-05-06

申请人： Wesley B. Bruce ,  Lisa J. Newman ,  Hajime Sakai

发明人： Wesley B. Bruce ,  Lisa J. Newman ,  Hajime Sakai

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/29

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： Methods and compositions for modulating development and/or developmental pathways are provided. The compositions of the present invention comprise nucleic acid and amino acid sequences. Particularly, the nucleotide sequence (SEQ ID NO:1, 3, 4 or 7) and amino acid sequence (SEQ ID NO:2 or 8) for a maize CLAVATA3-like (CLV3-like) polypeptide are provided. Methods are provided for the expression of the CLV3-like sequences in a host plant or plant cell to modulate plant development and/or developmental pathways. The methods of the invention find use in controlling or modulating cell division, differentiation and development. In particular, the sequences of the present invention find use in modulating meristem development. Transformed plants, plant cells, tissues, and seed are also provided.

摘要翻译： 提供了用于调节发育和/或发育途径的方法和组合物。 本发明的组合物包含核酸和氨基酸序列。 特别地，提供了玉米CLAVATA3样（CLV3样）多肽的核苷酸序列（SEQ ID NO：1,3,4或7）和氨基酸序列（SEQ ID NO：2或8）。 提供了用于在宿主植物或植物细胞中表达CLV3样序列以调节植物发育和/或发育途径的方法。 本发明的方法用于控制或调节细胞分裂，分化和发育。 特别地，本发明的序列用于调节分生组织发育。 还提供了转化的植物，植物细胞，组织和种子。

公开(公告)号：US06368806B1

公开(公告)日：2002-04-09

申请号：US09679769

申请日：2000-10-05

申请人： Steve Openshaw ,  Wesley B. Bruce

发明人： Steve Openshaw ,  Wesley B. Bruce

IPC分类号： C12Q168

CPC分类号： G06F19/18 ,  C12Q2600/158 ,  G06F19/20

摘要： The invention provides a method of associating a gene or an expression product with a complexly inherited phenotypic trait of interest in a plant. Plants are segregated by the presence or absence of a genetic marker. One or more of the segregated groups are expression profiled to determine the gene associated with the phenotypic trait of interest. The gene associated with the phenotypic trait of interest can be identified and/or isolated.

摘要翻译： 本发明提供了将基因或表达产物与植物中感兴趣的复合遗传表型性状相关联的方法。 通过遗传标记的存在或不存在分离植物。 一个或多个分离组是表达谱，以确定与感兴趣的表型性状相关的基因。 可以鉴定和/或分离与感兴趣的表型性状相关的基因。