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36 条记录 (耗时 0.021 秒)

    Material and method for colorimetric detection of small-molecule targets
    11.
    发明授权
    Material and method for colorimetric detection of small-molecule targets 有权

    公开(公告)号：US10725058B2

    公开(公告)日：2020-07-28

    申请号：US16179336

    申请日：2018-11-02

    申请人： Yi Xiao ,  Yingping Luo ,  Haixiang Yu

    发明人： Yi Xiao ,  Yingping Luo ,  Haixiang Yu

    IPC分类号： G01N33/94 ,  G01N33/52

    摘要： The subject invention provides methods, assays, and products for visual detection of small-molecule targets in a sample in both clinical and field settings within minutes. The subject invention is based on an aptamer sensor that reports the presence of small-molecule target via a sensitive colorimetric signal for naked-eye detection. The aptamer sensor is a CBSAzyme-based sensor having both target-mediated cooperative behavior of the CBSA and peroxidase-mimicking catalytic activity of DNAzyme. The subject invention also provides methods of using the CBSAzyme-based sensor.

    Aptamers that bind to natural and synthetic cannabinoids
    12.
    发明授权
    Aptamers that bind to natural and synthetic cannabinoids 有权

    公开(公告)号：US10907163B1

    公开(公告)日：2021-02-02

    申请号：US16901723

    申请日：2020-06-15

    申请人： Yi Xiao ,  Haixiang Yu

    发明人： Yi Xiao ,  Haixiang Yu

    IPC分类号： C12N15/11 ,  C12N15/115 ,  G01N33/94 ,  G01N21/64

    摘要： The subject invention provides materials and methods for single-step detection of small molecules, e.g., natural and synthetic cannabinoids, in a sample. The subjection invention provides nucleic acids materials, e.g., aptamers (nucleic acid oligonucleotides) that can bind to natural and/or synthetic cannabinoids. The method for detecting a natural or synthetic cannabinoid in a sample comprises contacting the sample with an aptamer-based sensor selective for the natural or synthetic cannabinoid, and sensitively and rapidly detecting the natural or synthetic cannabinoid in the sample. The aptamer-based sensor comprises aptamers that can specifically binds to natural and/or synthetic cannabinoids with nanomolar dissociation constant.

    Materials and methods for rapid and specific detection of synthetic cathinones
    14.
    发明授权
    Materials and methods for rapid and specific detection of synthetic cathinones 有权

    公开(公告)号：US10683507B2

    公开(公告)日：2020-06-16

    申请号：US15900061

    申请日：2018-02-20

    申请人： Yi Xiao ,  Haixiang Yu

    发明人： Yi Xiao ,  Haixiang Yu

    IPC分类号： C12N15/11 ,  C12N15/115 ,  C09B23/01 ,  C07C225/10 ,  G01N33/50

    摘要： The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.

    Materials and methods for rapid and sensitive detection of small-molecule targets
    15.
    发明授权
    Materials and methods for rapid and sensitive detection of small-molecule targets 有权

    公开(公告)号：US10550395B2

    公开(公告)日：2020-02-04

    申请号：US15947315

    申请日：2018-04-06

    申请人： Yi Xiao ,  Haixiang Yu

    发明人： Yi Xiao ,  Haixiang Yu

    IPC分类号： C12N15/115 ,  G01N33/94 ,  G01N33/92

    摘要： The subject invention provides methods, assays and products for detecting small-molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target in a sample comprises providing a sample, contacting the sample with an aptamer-based sensor selective for the small-molecule target, and sensitively and rapidly detecting the small-molecule target in the sample. Specifically, the method utilizes EATR-amplified small-molecule sensors based on cooperative binding split aptamers (CBSAs).

    Highly conductive porous paper-based metal thin films
    16.
    发明授权
    Highly conductive porous paper-based metal thin films 有权

    公开(公告)号：US10290386B2

    公开(公告)日：2019-05-14

    申请号：US15146440

    申请日：2016-05-04

    申请人： Yi Xiao ,  Bhargav Guntupalli ,  Janak Paudyal

    发明人： Yi Xiao ,  Bhargav Guntupalli ,  Janak Paudyal

    IPC分类号： H01B1/02 ,  H01B1/04

    摘要： A porous conductive thin film includes a layer of metal nanoparticles decorated on a layer of conductive carbon nanomaterials. The thin film can be supported by a porous support. The porous support can be a MCE paper upon which a metallic or semi-metallic single-walled carbon nanotube (SWCNT) layer is decorated with gold nanoparticles (AuNPs) or platinum nanoparticles (PtNPs). The thin film can be constructed by filtering a dispersion of SWCNTs onto MCE filter paper followed by the filtration of a citrate stabilized dispersion of AuNPs or PtNPs onto the SWCNT layer.

    Electronic device and method for operating same
    17.
    发明授权
    Electronic device and method for operating same 有权
    电子设备及其操作方法

    公开(公告)号：US08957765B2

    公开(公告)日：2015-02-17

    申请号：US13814910

    申请日：2011-08-24

    申请人： Xiaobing Guo ,  Yi Xiao ,  Wei Xie ,  Dengfeng Wu

    发明人： Xiaobing Guo ,  Yi Xiao ,  Wei Xie ,  Dengfeng Wu

    IPC分类号： G05B11/01 ,  G08C19/16 ,  G08C17/00

    CPC分类号： G08C19/16 ,  G08C17/00

    摘要： An electronic device (100) and method for operating the same. The electronic device (100) comprises at least two working modes and comprises a connection state acquisition unit (110) and a mode switching unit (12). The connection state acquisition unit (110) is used for acquiring the connection state between the electronic device (100) and a remote control unit, and the remote control unit is used for remotely controlling the electronic device (100). The mode switching unit (12) is used for switching the working mode of the electronic device (100) according to the connected state.

    摘要翻译： 一种电子设备（100）及其操作方法。 电子设备（100）包括至少两个工作模式，并且包括连接状态获取单元（110）和模式切换单元（12）。 连接状态获取单元（110）用于获取电子设备（100）和遥控单元之间的连接状态，并且远程控制单元用于远程控制电子设备（100）。 模式切换单元（12）用于根据连接状态切换电子设备（100）的工作模式。

    Target Detection Using a Single-Stranded, Self-Complementary, Triple-Stem DNA Probe
    18.
    发明申请
    Target Detection Using a Single-Stranded, Self-Complementary, Triple-Stem DNA Probe 审中-公开
    使用单链，自我互补的三重DNA探针进行目标检测

    公开(公告)号：US20110256637A1

    公开(公告)日：2011-10-20

    申请号：US13123577

    申请日：2009-10-13

    申请人： Hyongsok Soh ,  Yi Xiao

    发明人： Hyongsok Soh ,  Yi Xiao

    IPC分类号： G01N33/53 ,  C07H21/00 ,  C40B40/06

    CPC分类号： C12Q1/6827 ,  C12Q1/6818 ,  C12Q1/6839 ,  C12Q2565/607 ,  C12Q2565/519 ,  C12Q2537/119 ,  C12Q2565/1015

    摘要： Provided are novel single-stranded oligonucleotide probes that have a triple-stem configuration in the absence of target binding to the target binding sequence. The probes also have a fluorophore and a quencher. In the absence of target binding to the target binding sequence, these single-stranded oligonucleotide probes are capable of forming self-complementary duplexes such that the probe is in the triple-stem configuration and the fluorophore is positioned adjacent the quencher. In the presence of target binding to the target binding sequence, formation of the self-complementary duplexes is inhibited such that the probe is configured to position the fluorophore away from the quencher such that a signal of the fluorophore is detectable. Also provided are methods of using the probes.

    摘要翻译： 提供的是在不与靶结合序列靶结合的情况下具有三原子构型的新型单链寡核苷酸探针。 探针也具有荧光团和猝灭剂。 在目标结合靶结合序列不存在的情况下，这些单链寡核苷酸探针能够形成自身互补的双链体，使得探针处于三重茎构型并且荧光团邻近猝灭剂定位。 在目标结合靶结合序列的存在下，抑制自身互补双链体的形成，使得探针被配置为使荧光团远离猝灭剂使得荧光团的信号是可检测的。 还提供了使用探针的方法。