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公开(公告)号：US20080242624A1
公开(公告)日：2008-10-02
申请号：US11560550
申请日：2006-11-16
申请人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
发明人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
IPC分类号： A61K31/711 , A61P43/00
CPC分类号： C12N15/8209 , C07K14/415 , C12N15/8201 , C12N15/8206 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated polynucleotides, specifically Cyclin D polynucleotides, and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants for the purpose of increasing transformation efficiency.
摘要翻译： 本发明提供分离的多核苷酸，特别是细胞周期蛋白D多核苷酸及其编码的参与细胞周期调节的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了改变植物的细胞周期蛋白质含量和/或组成以提高转化效率的方法和组合物。
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公开(公告)号：US07256322B2
公开(公告)日：2007-08-14
申请号：US10744572
申请日：2003-12-23
申请人： Keith S. Lowe , Rebecca E. Cahoon , Christopher J. Scelonge , Yumin Tao , William J. Gordon-Kamm , Wesley B. Bruce , Lisa J. Newman
发明人： Keith S. Lowe , Rebecca E. Cahoon , Christopher J. Scelonge , Yumin Tao , William J. Gordon-Kamm , Wesley B. Bruce , Lisa J. Newman
CPC分类号： C12N15/8207 , C07K14/415 , C12N15/8201 , C12N15/821 , C12N15/8241 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。
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公开(公告)号：US06518487B1
公开(公告)日：2003-02-11
申请号：US09398858
申请日：1999-09-20
申请人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , George J. Hoerster , John A. McElver
发明人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , George J. Hoerster , John A. McElver
IPC分类号： A01H500
CPC分类号： C12N15/8209 , C07K14/415 , C12N15/8201 , C12N15/8206 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated polynucleotides and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants.
摘要翻译： 本发明提供参与细胞周期调节的分离的多核苷酸及其编码的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了关于改变细胞周期蛋白质含量和/或植物组成的方法和组合物。
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公开(公告)号：US08586361B2
公开(公告)日：2013-11-19
申请号：US12893418
申请日：2010-09-29
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US20110047655A1
公开(公告)日：2011-02-24
申请号：US12893418
申请日：2010-09-29
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US07223597B2
公开(公告)日：2007-05-29
申请号：US10167015
申请日：2002-06-11
申请人： Carl R. Simmons , William J. Gordon-Kamm , Gurmukh S. Johal , Pedro A. Navarro Acevedo , Yumin Tao
发明人： Carl R. Simmons , William J. Gordon-Kamm , Gurmukh S. Johal , Pedro A. Navarro Acevedo , Yumin Tao
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8263 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins that act as cell death inhibitors and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts, and antibody compositions.
摘要翻译： 本发明提供了作为细胞死亡抑制剂的分离的核酸及其编码的蛋白质及其使用方法。 本发明进一步提供表达盒，转化的宿主细胞，转基因植物和植物部分以及抗体组合物。
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公开(公告)号：US08318493B2
公开(公告)日：2012-11-27
申请号：US12756211
申请日：2010-04-08
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US20100192263A1
公开(公告)日：2010-07-29
申请号：US12756211
申请日：2010-04-08
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US09234194B2
公开(公告)日：2016-01-12
申请号：US12706489
申请日：2010-02-16
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 还提供了包含FRT位点的文库群的试剂盒，以及制备修饰的FRT重组位点的文库的方法。 重组生成修饰的FRT重组位点可用于多种目标多重核苷酸重组方法。
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公开(公告)号：US07736897B2
公开(公告)日：2010-06-15
申请号：US11487300
申请日：2006-07-14
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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