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公开(公告)号：US09234194B2
公开(公告)日：2016-01-12
申请号：US12706489
申请日：2010-02-16
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 还提供了包含FRT位点的文库群的试剂盒，以及制备修饰的FRT重组位点的文库的方法。 重组生成修饰的FRT重组位点可用于多种目标多重核苷酸重组方法。
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公开(公告)号：US08318493B2
公开(公告)日：2012-11-27
申请号：US12756211
申请日：2010-04-08
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US20100192263A1
公开(公告)日：2010-07-29
申请号：US12756211
申请日：2010-04-08
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US07736897B2
公开(公告)日：2010-06-15
申请号：US11487300
申请日：2006-07-14
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US08586361B2
公开(公告)日：2013-11-19
申请号：US12893418
申请日：2010-09-29
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US20110047655A1
公开(公告)日：2011-02-24
申请号：US12893418
申请日：2010-09-29
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US20080216198A1
公开(公告)日：2008-09-04
申请号：US12054556
申请日：2008-03-25
申请人： Zuo-Yu Zhao , Dennis L. Bidney , Evan D. Elsing , Michael D. Miller , Xinli E. Wu , William J. Gordon-Kamm
发明人： Zuo-Yu Zhao , Dennis L. Bidney , Evan D. Elsing , Michael D. Miller , Xinli E. Wu , William J. Gordon-Kamm
CPC分类号： C12N15/8241 , A01H5/10 , C12N15/8201 , C12N15/829
摘要： Methods for producing homozygous plants, seeds, and plant cells are provided. Also provided are methods of transformation.
摘要翻译： 提供了用于产生纯合植物，种子和植物细胞的方法。 还提供了转化的方法。
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公开(公告)号：US06512165B1
公开(公告)日：2003-01-28
申请号：US09613094
申请日：2000-07-10
申请人： Margit C. Ross , Laura A. Church , Patrea M. Hill , William J. Gordon-Kamm , Keith S. Lowe , George J. Hoerster , Dennis L. Bidney
发明人： Margit C. Ross , Laura A. Church , Patrea M. Hill , William J. Gordon-Kamm , Keith S. Lowe , George J. Hoerster , Dennis L. Bidney
IPC分类号： A01H500
CPC分类号： C12N15/8201 , C12N15/8205 , C12N15/8207
摘要： The invention provides improved plant transformation methods. In particular the method provides increased transformation freequency, especially in recalcitrant plants. The method comprises stably transforming a target cell with at least one polynucleotide of interest. The target cell has been previously transformed to stimulate growth of the cell and has gone through at least one cell division.
摘要翻译： 本发明提供改进的植物转化方法。 特别地，该方法提供增加的转化自由度，特别是在顽固性植物中。 该方法包括用至少一种目的多核苷酸稳定转化靶细胞。 目标细胞之前已经被转化以刺激细胞的生长并已经经历了至少一个细胞分裂。
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公开(公告)号：US06337100B1
公开(公告)日：2002-01-08
申请号：US09586959
申请日：2000-06-05
IPC分类号： A23D700
CPC分类号： C12N9/0083 , C12N15/8247
摘要： Antisense expression of a stearoyl-ACP desaturase gene in sunflower results in more than a four-fold increase in seed stearate, and can enhance palmitate content as well. Thus, sunflower oil containing over 40 percent saturated fatty acids can be produced, which is desirable in the context of various products, such as coating fat, margarine, soap and shortening.
摘要翻译： 向日葵中的硬脂酰-ACP去饱和酶基因的反义表达导致硬脂酸硬脂酸酯的增加超过4倍，并且还可以提高棕榈酸酯含量。 因此，可以生产含有超过40％的饱和脂肪酸的向日葵油，这在各种产品的情况下是可取的，例如包衣脂肪，人造奶油，肥皂和起酥油。
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10.发明申请Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange 审中-公开提高位点特异性多核苷酸交换效率的方法和组合公开(公告)号：US20070143881A1
公开(公告)日：2007-06-21
申请号：US11427947
申请日：2006-06-30
申请人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
发明人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
IPC分类号： A01H1/00
CPC分类号： C12N15/8213 , C12N15/8205 , C12N15/8207
摘要： Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.
摘要翻译： 使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。 所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中，将包含位点特异性重组系统的至少一个组分的组合物注入胚囊，提供更好的表达效率 ，重组，整合，交换，切除和/或转化感兴趣的多核苷酸。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳以及由其产生的组织，植物部分和/或植物的基因组中。 还提供了通过方法产生并包含感兴趣的多核苷酸的细胞，卵细胞，合子，胚胎，胚乳，组织，种子和/或植物。
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