AHAS MUTANTS
    6.
    发明申请
    AHAS MUTANTS 审中-公开
    AHAS MUTANT

    公开(公告)号:US20100287641A1

    公开(公告)日:2010-11-11

    申请号:US12594425

    申请日:2008-04-03

    摘要: The invention provides nucleic acids encoding mutants of the acetohydroxyacid synthase (AHAS) large subunit comprising at least two mutations, for example double and triple mutants, which are useful for producing transgenic or non-transgenic plants with improved levels of tolerance to AHAS-inhibiting herbicides. The invention also provides expression vectors, cells, plants comprising the polynucleotides encoding the AHAS large subunit double and triple mutants, plants comprising two or more AHAS large subunit single mutant polypeptides, and methods for making and using the same.

    摘要翻译: 本发明提供编码乙酰羟酸合酶(AHAS)大亚基的突变体的核酸,其包含至少两个突变,例如双重和三重突变体,其可用于产生具有改善的对AHAS抑制性除草剂的耐受性的转基因或非转基因植物 。 本发明还提供了表达载体,细胞,包含编码AHAS大亚基双重和三重突变体的多核苷酸的植物,包含两个或更多个AHAS大亚单位单突变体多肽的植物及其制备和使用的方法。

    Transposable element-anchored, amplification method for isolation and identification of tagged genes
    7.
    发明授权
    Transposable element-anchored, amplification method for isolation and identification of tagged genes 失效
    可转移元件锚定,用于分离和鉴定标记基因的扩增方法

    公开(公告)号:US06881539B1

    公开(公告)日:2005-04-19

    申请号:US09622353

    申请日:1999-02-16

    IPC分类号: C12P19/34 C12Q1/68

    摘要: A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.

    摘要翻译: 提供了用于快速分离和鉴定转座元件标记基因的DNA序列的方法。 该方法包括使用可转座元件锚定扩增来鉴定和克隆与突变表型相关的扩增产物的修饰AFLP方法。 一旦被克隆,可以使用目的扩增产物直接筛选cDNA文库或进行测序,并与序列同源性的可用数据库进行比较。 该方法的修改提供了用于将额外类型的插入事件的位置识别到基因组DNA中的方法,更具体地说是将基因插入到宿主生物体的基因组中。