公开(公告)号：US20220220370A1

公开(公告)日：2022-07-14

申请号：US17611896

申请日：2020-07-29

Applicant: Academia Sinica

Inventor： Michael HSIAO ,  Ming-Hsien CHAN ,  Subbiramaniyan KUBENDHIRAN ,  Ming-Che HSIEH ,  Zhen BAO ,  An-Bang WANG ,  Ru-Shi LIU

IPC: C09K11/02 ,  C09K11/56 ,  C09K11/77 ,  C09K11/70 ,  C09K11/88 ,  G01N33/58

Abstract: Disclosed herein is a nanocomposite comprising a core-shell nanoparticle and a core-shell quantum dot. The core-shell nanoparticle comprises a phosphor core, a shell layer, and a cleavable peptide. The core-shell quantum dot comprises a center core, an intermediate layer, an outer layer, a silica layer, and an arginylglycylaspartic acid (RGD) peptide. The core-shell nanoparticle and the core-shell quantum dot are linked to each other via forming a peptide bond between the cleavable peptide and the RGD peptide. Also disclosed are the uses of the nanocomposite in making a diagnosis of tumors.

公开(公告)号：US20220218814A1

公开(公告)日：2022-07-14

申请号：US17609822

申请日：2020-05-08

Applicant: ACADEMIA SINICA ,  NATIONAL TAIWAN UNIVERSITY

Inventor： Che-Ming Jack HU ,  Hung-Chih YANG

IPC: A61K39/12 ,  A61K39/39 ,  A61K9/51

Abstract: We utilized a biocompatible hollow polymeric nanoparticle that coencapsulates T cell epitope peptides and oligodeoxynucleotide (ODN) CpG, and designed immunization strategies to evaluate its protectivity against influenza viruses in mice. This nanoparticle-based peptide vaccine adjuvanted with CpG stimulated robust antigen-specific CD4 and CD5 T cell immunity, but only caused minimal adverse effects compared with crude mixture of peptides and CpG. We used two peptides derived from the nucleocapsid protein (NP), MHC class I-restricted NP366-374 and MHC class ll-restricted NP311-325. This novel nanoparticle vaccine with two epitope peptides plus CpG induced robust and fully protective T cell immunity against influenza viruses. We demonstrates the utility of this novel hollow nanoparticle with co-encapsulation of only a pair of CD4+ and CD8+ T cell-stimulating influenza viral peptides and CpG in establishing near-sterilizing protective resident T cell immunity against heterosubtypic IAV infections, a critical step towards the development of universal influenza T cell vaccines.

公开(公告)号：US20220213188A1

公开(公告)日：2022-07-07

申请号：US17269991

申请日：2019-09-25

Applicant: Academia Sinica

Inventor： Shie-Liang HSIEH ,  Pei-Shan SUNG ,  Ming-Ting HUANG ,  An-Suei YANG ,  Chung-Ming YU

IPC: C07K16/28 ,  A61P31/20 ,  A61P25/28

Abstract: Provided herein is a method for treating neurodegenerative diseases, such as Alzheimer's disease (AD), by use of monoclonal antibody, which exhibits a binding affinity to Siglec-3 receptor. According to some embodiments of the present disclosure, the monoclonal antibody is capable of enhancing phagocytosis of neurotoxic peptides by immune cells thereby providing a neuroprotective effect to a subject in need thereof.

公开(公告)号：US11339405B2

公开(公告)日：2022-05-24

申请号：US15951114

申请日：2018-04-11

Applicant: Academia Sinica

Inventor： Hung-Chih Kuo ,  Ching-Yu Chuang ,  Chan-Hsien Yeh

IPC: C12N15/85 ,  G01N33/50 ,  C12N5/079 ,  A61K35/36 ,  C12N5/09 ,  C12N5/0793 ,  C12N5/0797 ,  A61K35/30 ,  C12N15/86 ,  C12N5/077

Abstract: Disclosed herein are kits comprising transcription factors for inducing a fibroblast cell into an induced embryonic neural progenitor cell. The induced embryonic neural progenitor cell is then capable of differentiating into an astrocyte, an oligodendrocyte or a neuron. Also disclosed are the uses of the kit as a platform for selecting a drug candidate to treat neurological diseases.

公开(公告)号：US20220120757A1

公开(公告)日：2022-04-21

申请号：US17502399

申请日：2021-10-15

Applicant: ACADEMIA SINICA

Inventor： Huan-Cheng CHANG ,  Yuen-Yung HUI ,  Hsin-Hung LIN ,  Oliver Y. CHEN

IPC: G01N33/58 ,  G01N33/543 ,  G01N21/64

Abstract: The present disclosure discloses a detection method and a detection system for detecting an object of interest in a biological sample. The detection method comprises the following steps: providing a detection kit comprising a detection carrier and a reporter, wherein the detection carrier comprises a coating protein having a recognition site for binding the object of interest, the reporter including a spin luminescent material; mixing the reporter and the biological sample and loading the mixture onto the detection carrier; placing the detection carrier with the mixture of the reporter molecule and the biological sample in a time-varied physical field; irradiating a detection carrier placed in the time-varied physical field with an excitation light to excite the spin luminescent material to generate a fluorescence signal modulated by the time-varied physical field; and receiving and analyzing the fluorescence signal.

公开(公告)号：US11286304B2

公开(公告)日：2022-03-29

申请号：US16653986

申请日：2019-10-15

Applicant: Academia Sinica

Inventor： Fu-Tong Liu ,  Hung-Lin Chen ,  Po-Cheng Chiang

IPC: C07K16/28 ,  G01N33/50 ,  A61P17/06 ,  A61P35/00

Abstract: Provided herein is a novel antibody exhibiting binding affinity and specificity to galectin-7. Also provided herein are methods for treating psoriasis or cancer in a subject by administering to the subject a drug selected with the aid of the present anti-galectin-7 antibody.

公开(公告)号：US11266603B2

公开(公告)日：2022-03-08

申请号：US16455800

申请日：2019-06-28

Applicant: Academia Sinica

Inventor： Hsien-Ming Lee ,  Hua-De Gao ,  Jia-Lin Hong ,  Chih-Yu Kuo ,  Cheng-Bang Jian

IPC: A61K9/127 ,  C07K14/00 ,  A61K41/00 ,  A61K49/00 ,  C07K7/08 ,  A61K31/704

Abstract: Disclosed herein are novel synthetic polypeptides and uses thereof in the preparation of liposomes. According to embodiments of the present disclosure, the synthetic polypeptide comprises a membrane lytic motif, a masking motif, and a linker configured to link the membrane lytic motif and the masking motif. The linker is cleavable by a stimulus, such as, light, protease, or phosphatase. Once being coupled to a liposome, the exposure to the stimulus cleaves the linker that results in the separation of the masking motif from the membrane lytic motif, which in turn exerts membrane lytic activity on the liposome that leads to the collapse of the intact structure of the liposome, and releases the agent encapsulated in the liposome to the target site. Also disclosed herein are methods of diagnosing or treating a disease in a subject by use of the present liposomes.

公开(公告)号：US11265449B2

公开(公告)日：2022-03-01

申请号：US16013663

申请日：2018-06-20

Applicant: ACADEMIA SINICA

Inventor： Jung-Chi Liao ,  Yi-De Chen ,  Chih-Wei Chang ,  Weng Man Chong

IPC: H04N5/225 ,  G06K9/00 ,  G02B21/36 ,  G02B21/26 ,  G02B21/06 ,  H04N5/235 ,  G06K9/32 ,  G06K9/20

Abstract: A microscope-based system and a method for image-guided microscopic illumination are provided. The microscope-based system for image-guided microscopic illumination comprises a microscope, an illuminating assembly, an imaging assembly, a first processing module, and a second processing module. The microscope comprises a stage, and the stage is configured to be loaded with a sample. The imaging assembly comprises a camera. The processing modules are coupled to the microscope, the imaging assembly, and the illuminating assembly. A processing module controls the imaging assembly such that the camera acquires an image or images of the sample of a first field of view, and the image or images are automatically transmitted to a processing module and processed by the first processing module automatically in real-time based on a predefined criterion, so as to determine an interested region in the image and so as to obtain a coordinate information regarding to the interested region, and the coordinate information regarding to the interested region is transmitted to a processing module, where this module controls the illuminating assembly to illuminate the interested region of the sample according to the received coordinate information regarding to the interested region. It is therefore an object to process the proteins, lipids, nucleic acids, or biochemical species in an area of interest specified by fluorescent signals or structural signature of biological images. Additional object is to collect a large amount of proteins, lipids, and nucleic acids through high content labeling and purification in order to identify proteins, lipids, nucleic acids, or biochemical species of interest in the area of interest by analytical approaches including mass spectrometry and sequencing.

公开(公告)号：US20220011646A1

公开(公告)日：2022-01-13

申请号：US17281814

申请日：2019-10-01

Applicant: William Marsh Rice University ,  Academia Sinica

Inventor： Michael Semmlinger ,  Ming Lun Tseng ,  Jian Yang ,  Ming Zhang ,  Din Ping Tsai ,  Liangliang Dong ,  Arash Ahmadivand ,  Peter Nordlander ,  Nancy Jean Halas

IPC: G02F1/37 ,  G02F1/35

Abstract: A harmonic light-generating metasurface includes a base substrate and a plurality of structures, that include nonlinear material, that are disposed in a pattern on a surface of the base substrate. Each structure of the plurality of structures individually supports a magnetic dipole mode. An electromagnetic field enhancement of the magnetic dipole mode induces generation of a harmonic signal by the plurality of structures. Alternatively, a harmonic light-generating metasurface, includes a base substrate, a supporting substrate that includes a nonlinear material, and a plurality of paired structures disposed in a pattern on a surface of the supporting substrate. Each paired structure, of the plurality of paired structures, collectively supports a toroidal dipole mode. An electromagnetic field enhancement of the toroidal dipole mode penetrates the supporting substrate to induce generation of a harmonic signal by the supporting substrate.

公开(公告)号：US20210320623A1

公开(公告)日：2021-10-14

申请号：US17273868

申请日：2019-12-06

Applicant: ACADEMIA SINICA

Inventor： YI-CHING WU ,  YUH-JING HWANG ,  Chau-Ching Chiong ,  Bo-Ze Lu ,  HUEI WANG

IPC: H03D7/12

Abstract: The present invention is to provide a mixing circuit, comprising: a first transistor; a second transistor; a third transistor; a first connection point connected to a gate terminal of the first transistor, a drain terminal of the second transistor and a source terminal of the third transistor; a second connection point connected to a source terminal of the first transistor and a gate terminal of the second transistor; and a third connection point connected to a drain terminal of the first transistor and a drain terminal of the third transistor.