公开(公告)号：US20150313216A1

公开(公告)日：2015-11-05

申请号：US14654024

申请日：2013-12-26

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA- EMBRAPA ,  THE UNIVERSITY OF MELBOURNE

Inventor： Thales Lima Rocha ,  Raphael Garcia de Sousa Evaristo ,  Maria Fátima Grossi de Sá ,  Djair Dos Santos de Lima e Souza ,  Maria Cristina Mattar da Silva ,  Vera Lúcia Perussi Polez ,  Ute Roessner ,  Tony Bacic

IPC: A01N37/06 ,  A01N37/44 ,  A01N35/02

CPC classification number: A01N37/06 ,  A01N35/02 ,  A01N37/44 ,  A01N65/20 ,  A01N37/02 ,  A01N43/16

Abstract: The present invention relates generically to compositions used for controlling agricultural pests and, in a particular embodiment, to compositions usable for combating nematodes.The invention presents a feasible alternative to the use of nematotoxic compounds that are harmful to the environment and that may damage human health. This alternative is embodied through a nematotoxic composition for controlling nematodes and that may be used on plants, parts of plants and on the soil. The respective technology results from unexpected results achieved from experiments carried out with extracts of the plant Canavalia ensiformis, resulting in an unheard-of nematotoxic composition comprising a specific combination of substances that are naturally found in the extract and that act synergistically.

公开(公告)号：US20230201849A1

公开(公告)日：2023-06-29

申请号：US17384063

申请日：2020-01-24

Applicant: Spraying Systems Co. ,  Embrapa

Inventor： Aldemir Chaim ,  Edson Francelino Ribeiro da Rocha

IPC: B05B5/00 ,  B05B5/04 ,  A01M7/00

CPC classification number: B05B5/007 ,  B05B5/0407 ,  B05B5/0415 ,  A01M7/0028

Abstract: An electrostatic centrifugal spraying system for direct electrification includes a tank configured to store liquid. The tank includes an internal dielectric surface and an external conducting surface, wherein when the liquid is stored in the tank, the tank is configured to act as a capacitor storing electrical energy for electrostatic spraying. The spraying system further includes a power supply, configured to electrify liquid drops of the stored liquid, and a spray nozzle, comprising a spray disk for blowing the electrified liquid drops unto a target.

公开(公告)号：US10961542B2

公开(公告)日：2021-03-30

申请号：US14778190

申请日：2014-03-18

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA—EMBRAPA ,  FUNDAÇÃO UNIVERSIDADE DE BRASILIA—FUB—UNB

Inventor： Juliana Dantas de Almeida ,  Leila Maria Gomes Barros ,  Renata Henrique Santana ,  Ricardo Vilela Abdelnoor ,  Felipe Rodrigues da Silva

IPC: C12N15/82

Abstract: The present invention relates to a new promoter of the expression of genes in plants. More specifically, the invention relates to regulatory sequences of polynucleotides that are isolated from soy plants and can initiate and activate polynucleotide transcription, and to the use of these regulatory sequences to modify the transcription of endogenous and/or heterologous polynucleotides and to produce polypeptides. The invention further describes DNA constructs that contain the promoter of the gene of the supposed flavonol-sulfotransferase protein in soy plants, which promoter is functionally linked to a heterologous and/or endogenous gene. Moreover, the invention relates to the use of these constructs in the form of expression cassettes, expression vectors, recombinant vectors and in plants, plant cells or transgenic protoplasts. The invention also describes a method using such constructs that contain the promoter of the gene of the putative flavonol-sulfotransferase protein of soy plants for the production of transgenic plants, plant cells or protoplasts.

公开(公告)号：US10365196B2

公开(公告)日：2019-07-30

申请号：US14786480

申请日：2014-04-24

Applicant: Empresa Brasileira de Pesquisa Agropecuaria—Embrapa ,  Tecnicer Tecnologia Ceramica LTDA

Inventor： Adonai Gimenez Calbo ,  Carlos Manoel Pedro Vaz ,  Waldir Aparecido Marouelli ,  Luis Fernando Porto

IPC: G01N13/02 ,  G01N7/10 ,  G01N33/24 ,  G01N7/00

Abstract: The present invention provides a water tension sensor with a non-sintered core that allows batches of similar and standard sensors to be manufactured in a simple manner, for operation with air and with an air flow outlet, which sensor can be used to read water tension and trigger dripping. The sensor according to the invention is ideal for manually reading soil water tension and for automating irrigation systems based on a static pressure or gas propulsion mechanism.

公开(公告)号：US09750247B2

公开(公告)日：2017-09-05

申请号：US14654017

申请日：2013-12-26

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA—EMBRAPA ,  THE UNIVERSITY OF MELBOURNE

Inventor： Thales Lima Rocha ,  Raphael Garcia de Sousa Evaristo ,  Maria Fátima Grossi de Sá ,  Djair dos Santos de Lima e Souza ,  Maria Cristina Mattar da Silva ,  Vera Lúcia Perussi Polez ,  Ute Roessner ,  Tony Bacic

IPC: A01N37/44 ,  A01N37/00 ,  A01N37/02 ,  A61K31/19 ,  A61K31/194 ,  A61K31/195 ,  A01N41/12 ,  A01N37/06 ,  A01N43/16

CPC classification number: A01N41/12 ,  A01N37/02 ,  A01N37/06 ,  A01N43/16 ,  A01N37/44

Abstract: The present invention relates generically to compositions used for controlling agricultural pests and, in a particular embodiment, to compositions usable for combating nematodes.The invention presents a feasible alternative to the use of nematotoxic compounds that are harmful to the environment and that may damage human health. This alternative is embodied through a nematotoxic composition for controlling nematodes and that may be used on plants, parts of plants and on the soil. The respective technology results from unexpected results achieved from experiments carried out with extracts of the plant Canavalia ensiformis, resulting in an unheard-of nematotoxic composition comprising a specific combination of substances that are naturally found in the extract and that act synergistically.

公开(公告)号：US20170029843A1

公开(公告)日：2017-02-02

申请号：US15105641

申请日：2014-12-18

Applicant: EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA ,  FUNDAÇÃO UNIVERSIDADE DE BRASÍLIA - FUB - UNB

Inventor： Maria Fátima GROSSI DE SA ,  Roberta RAMOS COELHO ,  Alexandre Augusto PEREIRA FIMINO ,  Leonardo LIMA PEPINO DE MACEDO ,  Maria Cristina MATTAR DA SILVA ,  Isabela TRISTAN LOURENÇO

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8286 ,  C12N15/111 ,  C12N15/1137 ,  C12N15/8218 ,  C12N2330/51 ,  Y02A40/162

Abstract: The present invention relates to the control of pest infestation by inhibiting or reducing the expression of genes of the family of chitin synthase and of vitellogenin, as well as through the expression of the toxin Cry8ka5. The invention further provides method and compositions for controlling pests, by feeding the pest with one or more double-stranded RNA molecules provided by the present invention, as well as double-stranded RNA molecule provided by the present invention, as well as through the action of the toxin Cry8ka5 on the target insect. The invention further describes a method of obtaining transgenic plants that express double-stranded RNA molecules and the toxin protein Cry8ka5. The present invention is preferably used for cotton plants.

Abstract translation: 本发明涉及通过抑制或降低几丁质合酶和卵黄发生素家族的基因表达以及通过毒素Cry8ka5的表达来控制害虫侵袭。 本发明进一步提供了通过用本发明提供的一种或多种双链RNA分子喂养害虫以及本发明提供的双链RNA分子以及通过动作来控制害虫的方法和组合物 的毒素Cry8ka5对目标昆虫。 本发明还描述了获得表达双链RNA分子和毒素蛋白Cry8ka5的转基因植物的方法。 本发明优选用于棉花植物。

公开(公告)号：US20160081351A1

公开(公告)日：2016-03-24

申请号：US14801998

申请日：2015-07-17

Applicant: The United States of America, as represented by the Secretary of Agriculture ,  Empresa Brasileira De Pesquisa Agropecuaria - Embrapa

Inventor： Mark A. Jackson ,  Gabriel M. Mascarin ,  Nilce Naomi Kobori

IPC: A01N63/04 ,  C05F11/08 ,  C12N1/14

CPC classification number: A01N63/04 ,  C05F11/08 ,  C12N1/04 ,  C12N1/14

Abstract: Disclosed is an invention that relates to the formation of microsclerotial propagules by mycoparasitic fungi and the use of those microsclerotia for plant disease control. Representative microsclerotia propagules formed are from fugal species Trichoderma harzianum, Trichoderma lignorum, Trichoderma viridae, Trichoderma reesei, Trichoderma koningii, Trichoderma pseudokoningii, Trichoderma polysporum, Trichoderma hamatum, and Trichoderma asperellum.

Abstract translation: 公开了涉及由真菌寄生真菌形成微生物繁殖体的发明，以及这些微生物用于植物病害防治的用途。 形成的代表性微视星体繁殖体是来自哈萨克木霉（Trichoderma harzianum），木霉（Trichoderma lignorum），木霉（Trichoderma viridae），里氏木霉（Trichoderma reesei），木霉木霉（Trichoderma koningii），木霉木霉（Trichoderma pseudokoningii），木霉（Trichoderma polyporum）），木霉木霉（Trichoderma hamatum）和木霉（Trichoderma asperellum）

公开(公告)号：US20150250129A1

公开(公告)日：2015-09-10

申请号：US14679647

申请日：2015-04-06

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA-EMBRAPA ,  BASF AGROCHEMICAL PRODUCTS B.V.

Inventor： Dale CARLSON ,  Francisco Jose Lima ARAGAO ,  Carlos Alberto Arrabal ARIAS ,  Luiz LOUZANO ,  Bruce M. LUZZI ,  Tim MALEFYT ,  Elibio Leopoldo Rech FILHO ,  Siyuan TAN ,  Adolfo ULBRICH ,  Tadashi YOTSUMOTO ,  Ute LINEMANN

IPC: A01H5/10 ,  C12Q1/68 ,  C12N15/82 ,  A01H1/04

CPC classification number: A01H5/10 ,  A01H1/04 ,  C12N15/8274 ,  C12N15/8275 ,  C12Q1/6895

Abstract: Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location comprises genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO:5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.

Abstract translation: 提供了与转基因AHAS抑制除草剂抗性大豆植物相关的组合物和方法。 提供了具有突变的AHAS编码序列的127种大豆植物，其赋予对AHAS抑制性除草剂的耐受性。 在确定的染色体位置上具有事件127核酸分子的127个大豆植物包括至少具有SEQ ID NO：5和/或6的核酸序列的基因组/转基因接头。事件的基因组插入位点的表征 127提供增强的育种效率并且能够使用分子标记来跟踪育种种群中的转基因插入物及其后代。 提供用于鉴定，检测和使用事件127种大豆植物的各种方法和组合物。

公开(公告)号：US20150197762A1

公开(公告)日：2015-07-16

申请号：US14391066

申请日：2013-04-09

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA

Inventor： Juliana Dantas Dealmeida ,  Leila Maria Gomes Barros ,  Mauro Carneiro ,  Alan Carvalho Andrade ,  Felipe Rodrigues Da Silva ,  Luiz Filipe Protasio Pereira ,  Michelle Guitton Cotta ,  Mirian Therezinha Souza Da Eira

IPC: C12N15/82

CPC classification number: C12N15/8234

Abstract: The present invention relates to a promoter sequence specific for expression of genes of interest in fruit endosperm. The invention further describes DNA constructs that contain the promoter, as well as the method using such constructs to produce transgenic plants, plant cells or protoplasts. The expression of the transgene only in the organ of interest enables accumulation of the exogenous transcript only in the fruit and constitutes an advantage over the constitutive expression, since it favors implementation of strategies that aim at obtaining cultivars having higher aggregate value like: adaptation to environmental stress, tolerance to pathogens, pests and agricultural defensives, increase in the nutritional and therapeutic value and a new alternative to systems of expression in plant organism, generating more competitive cultivars at lower costs. Besides, the present invention also presents a sequence with constitutive expression, providing an alternative to the implementation of expression system where the constitutive expression is required. The invention has the objective of increasing the economic, social and, environmental and biosafety benefits associated to the genetic transformation.

Abstract translation: 本发明涉及对果实胚乳中感兴趣的基因表达特异性的启动子序列。 本发明还描述了含有启动子的DNA构建体，以及使用这种构建体产生转基因植物，植物细胞或原生质体的方法。 仅在感兴趣的器官中转基因的表达使得外源转录物仅能够在果实中积累，并且构成了优于组成型表达的优点，因为它有利于实现旨在获得具有较高总体值的品种的策略，如：适应环境 胁迫，对病原体的耐受性，害虫和农业防御力，营养和治疗价值的增加，以及植物生物体内表达系统的新选择，以更低的成本产生更具竞争力的品种。 此外，本发明还提供了具有组成型表达的序列，为实现需要组成型表达的表达系统提供了替代方案。 本发明的目的是增加与遗传转化相关的经济，社会和环境以及生物安全方面的利益。

公开(公告)号：US20140086875A1

公开(公告)日：2014-03-27

申请号：US14092602

申请日：2013-11-27

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA ,  UNIVERSITY COLLEGE CARDIFF CONSULTANTS LIMITED ,  BTHEK BIOTECNOLOGIA LTDA.

Inventor： Rose Gomes Monnerat Solon DE PONTES ,  Carlos Marcelo Silveira SOARES ,  Colin BERRY

IPC: A01N63/00

CPC classification number: A01N63/00

Abstract: The objective of the current invention is the control of agricultural infestations based on alternative methods that are less aggressive to the environment and that are harmless to other invertebrates and to man; for instance biological control using bacterial strains belonging to species of the genera Bacillus, Brevibacillus and/or Paenibacillus. A first embodiment of the invention refers to compositions based on the systemic utilization of bacterial strains belonging to species of the genera Bacillus, Brevibacillus and/or Paenibacillus to protect plants by using the composition containing the above referred bacteria in wild-type, conjugated, mutant or transgenic forms and/or the substances produced by them. A second embodiment of the invention refers to a method of bioinsecticide application based on bacteria from the genera Bacillus, Brevibacillus and/or Paenibacillus. A third embodiment of the invention is related to the bioinsecticide usage of the formulations based on bacteria from the genera Bacillus, Brevibacillus and/or Paenibacillus.

Abstract translation: 本发明的目标是根据对环境侵略性较小并且对其他无脊椎动物和人类无害的替代方法来控制农业侵害; 例如使用属于芽孢杆菌属，短杆菌属和/或Paenibacillus属的物种的细菌菌株的生物控制。 本发明的第一个实施方案是指基于属于芽孢杆菌属，短杆菌属和/或Paenibacillus属的细菌菌株的系统利用的组合物，以通过在野生型，缀合的突变体中使用含有上述细菌的组合物来保护植物 或转基因形式和/或由它们产生的物质。 本发明的第二个实施方案是指基于来自芽孢杆菌属，短杆菌属和/或芽孢杆菌属的细菌的生物杀虫剂施用方法。 本发明的第三个实施方案涉及基于来自芽孢杆菌属，短杆菌属和/或Paenibacillus属的细菌的制剂的生物杀灭剂用途。