公开(公告)号：US20060194304A1

公开(公告)日：2006-08-31

申请号：US11412130

申请日：2006-04-27

Applicant: Metin Colpan ,  Peter Moritz ,  Joachim Schorr

Inventor： Metin Colpan ,  Peter Moritz ,  Joachim Schorr

IPC: C02F3/34

CPC classification number: B01D15/363 ,  B01D15/3804 ,  C12N15/101 ,  C12N15/87 ,  Y10S435/81 ,  Y10S435/82 ,  Y10S436/808 ,  Y10T436/10 ,  G01N30/461

Abstract: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural sources are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with affinity chromatographic methods; said fractions are preincubated with an aqueous salt solution and detergents, treated with anion exchange material and then washed with another salt solution, and the active ingredients are eluted from the anion exchanger, followed by further purification in a per se known manner.

公开(公告)号：US07074916B2

公开(公告)日：2006-07-11

申请号：US10340834

申请日：2003-01-13

Applicant: Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

Inventor： Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

IPC: C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12N15/1006 ,  C12N15/101 ,  C12Q1/6806

Abstract: The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.

公开(公告)号：US06609618B2

公开(公告)日：2003-08-26

申请号：US09900199

申请日：2001-07-09

Applicant: Metin Colpan

Inventor： Metin Colpan

IPC: B01D2900

CPC classification number: C12N15/101 ,  B01D39/00 ,  C12N15/1017 ,  Y10T436/25375 ,  Y10T436/255

Abstract: Described is a method of isolating cell components, such as nucleic acids, from natural sources by filtering a sample of the digested natural sources such as cells or cell fragments. The method is characterized in that the sample is passed through a filter, the pore size of which decreases in the direction of flow of the sample through the filter.

Abstract translation: 描述了通过过滤消化的天然来源（例如细胞或细胞碎片）的样品从天然来源分离细胞成分（例如核酸）的方法。 该方法的特征在于，样品通过过滤器，其孔径在样品通过过滤器的流动方向上减小。

公开(公告)号：US06383393B1

公开(公告)日：2002-05-07

申请号：US08392882

申请日：1996-03-15

Applicant: Metin Colpan ,  Joachim Schorr ,  Ralf Herrmann ,  Petra Feuser

Inventor： Metin Colpan ,  Joachim Schorr ,  Ralf Herrmann ,  Petra Feuser

IPC: B01D1508

CPC classification number: C12N15/101

Abstract: A method for the purification and separation of nucleic acid mixtures by chromatography including adsorbing the nucleic acids to be separated and purified from a solution with a high concentration of salts (ionic strength) and/or a high concentration of alcohol on a substrate and subsequent desorbing from the substrate by means of a solution with lower concentration of salts (ionic strength).

Abstract translation: 一种通过色谱法纯化和分离核酸混合物的方法，其包括将从高浓度盐（离子强度）和/或高浓度醇溶液中分离和纯化的核酸吸附在底物上并随后解吸 通过具有较低浓度盐的溶液（离子强度）从底物引发。

公开(公告)号：US06020186A

公开(公告)日：2000-02-01

申请号：US442406

申请日：1995-05-16

Applicant: Karsten Henco ,  Metin Colpan ,  Petra Feuser

Inventor： Karsten Henco ,  Metin Colpan ,  Petra Feuser

IPC: C12N15/10 ,  C12Q1/68 ,  C12N3/00

CPC classification number: C12N15/101 ,  C12N15/1006 ,  C12Q1/6806

Abstract: A device and a process for isolating nucleic acids by lysing intact cells and removing nucleic acids emerging from the lysed cells by the following steps:a) the cells are immobilized in a porous matrix, with the size of matrix voids being in the range of the type of cell to be lysed;b) the cells are lysed;c) the nucleic acids are fixated on the matrix surface, and subsequentlyd) are eluted.

Abstract translation: 通过以下步骤裂解完整细胞和从裂解的细胞中除去出现的核酸来分离核酸的装置和方法：a）将细胞固定在多孔基质中，其中基质空隙的大小在 待裂解的细胞类型; b）细胞裂解; c）将核酸固定在基质表面上，随后d）被洗脱。

公开(公告)号：US5464541A

公开(公告)日：1995-11-07

申请号：US153463

申请日：1993-11-17

Applicant: James E. Aysta ,  Metin Colpan

Inventor： James E. Aysta ,  Metin Colpan

IPC: B01D15/00 ,  B01D24/00 ,  B01D29/01 ,  B01D61/18 ,  B01L3/00 ,  B01L99/00 ,  C12M1/00 ,  C12Q1/68 ,  B01D29/00

CPC classification number: B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L3/50855 ,  Y10T436/25375 ,  Y10T436/255

Abstract: A device (10) for separating liquid samples has a sample container (12), bottom wall (20) of which has an outlet opening (22) provided therein. The outlet opening (22) is joined by an outlet spout (24) extending in the axial direction of the sample container (12). On bottom wall (20) there is a separation layer (28). Below sample container (12), a collecting container (32) is arranged abutting sample container (12) such that an exchange of air between the interior of the collecting chamber (32) and the environment is possible, yet an escape of liquid is largely inhibited. The contact surface between collecting chamber (32) and sample container (12) and end (26) of the outlet spout (24) through which the liquid is discharged, are axially spaced apart.

Abstract translation: 用于分离液体样品的装置（10）具有样品容器（12），其底壁（20）具有设置在其中的出口（22）。 出口开口（22）通过在样品容器（12）的轴向方向上延伸的出口喷口（24）连接。 在底壁（20）上有分离层（28）。 在样本容器（12）的下面，收集容器（32）设置成邻接样本容器（12），使得可以在收集室（32）的内部和环境之间交换空气，但是液体逸出很大程度上 抑制。 收集室（32）和样品容器（12）之间的接触表面以及排出液体的出口喷口（24）的端部（26）轴向间隔开。