公开(公告)号：US20060141558A1

公开(公告)日：2006-06-29

申请号：US11025177

申请日：2004-12-29

Applicant: Xiao-Song Tang ,  Qiong Cheng ,  Joanne Shyr ,  Luan Tao

Inventor： Xiao-Song Tang ,  Qiong Cheng ,  Joanne Shyr ,  Luan Tao

IPC: C12P23/00 ,  C07H21/04 ,  C12P21/06 ,  C12N9/02 ,  C12N1/18 ,  C12N15/74

CPC classification number: C12N9/0069 ,  C12N9/0071 ,  C12P23/00 ,  Y10S435/858

Abstract: Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts.

公开(公告)号：US20050148054A1

公开(公告)日：2005-07-07

申请号：US10476198

申请日：2002-05-03

Applicant: Wei Qi ,  Fateme Sariaslani ,  Xiao-song Tang

Inventor： Wei Qi ,  Fateme Sariaslani ,  Xiao-song Tang

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/88 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P13/22 ,  C12N1/18 ,  C12N9/06

CPC classification number: C12N9/88 ,  C12P7/40 ,  C12P7/42 ,  C12P13/225

Abstract: Genes encoding phenylalanine ammonia-lyase (PAL), tyrosine ammonia lyase (TAL) and phenylalanine hydroxylase (PAH) have been introduced into a host organism for the production of Para-hydroxycinnamic acid (PHCA). The introduction of these genes results in the redirection of carbon flow in the host, optimizing the flow of carbon from glucose to PHCA. The intermediates, tyrosine and cinnamic acid are also produced.

Abstract translation: 已经将编码苯丙氨酸氨裂解酶（PAL），酪氨酸氨裂解酶（TAL）和苯丙氨酸羟化酶（PAH）的基因导入宿主生物体中以产生对羟基肉桂酸（PHCA）。 这些基因的引入导致宿主中碳流的重定向，优化碳从葡萄糖到PHCA的流动。 还生产中间体，酪氨酸和肉桂酸。

公开(公告)号：US06368837B1

公开(公告)日：2002-04-09

申请号：US09627216

申请日：2000-07-27

Applicant: Anthony A. Gatenby ,  Sima Sariaslani ,  Xiao-Song Tang ,  Wei Wei Qi ,  Todd Vannelli

Inventor： Anthony A. Gatenby ,  Sima Sariaslani ,  Xiao-Song Tang ,  Wei Wei Qi ,  Todd Vannelli

IPC: C12P742

CPC classification number: C12N9/0042 ,  C12N9/0077 ,  C12N9/88 ,  C12N15/8243 ,  C12P7/42

Abstract: The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast Rhodotorula glutinis into E. coli underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast Rhodotorula glutinis plus the plant cytochrome P-450 and the cytochrome P-450 reductase into E. coli. In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.

Abstract translation: 本发明提供了几种生物制备对羟基肉桂酸（PHCA）的方法。 本发明还涉及具有将肉桂酸转化为PHCA的能力的新真菌和细菌的发现。 本发明涉及通过将来自酵母红酵母的野生型PAL掺入到大肠杆菌中的葡萄糖转化为PHCA的新的生物催化剂的开发，其强调野生型PAL将酪氨酸转化为PHCA的能力。 本发明还涉及开发新的生物催化剂，用于通过将来自酵母红酵母（Rhodotorula glutinis）加植物细胞色素P-450和细胞色素P-450还原酶的野生型PAL掺入大肠杆菌将葡萄糖转化为PHCA。 在另一个实施方案中，本发明提供了通过具有增强的酪氨酸氨裂解酶（TAL）活性的野生型酵母PAL的诱变来开发新的生物催化剂。