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    Method for production of C30-aldehyde carotenoids
    5.
    发明授权
    Method for production of C30-aldehyde carotenoids 失效
    生产C30-醛类胡萝卜素的方法

    公开(公告)号：US07098000B2

    公开(公告)日：2006-08-29

    申请号：US10860291

    申请日：2004-06-03

    申请人： Qiong Cheng ,  Luan Tao

    发明人： Qiong Cheng ,  Luan Tao

    IPC分类号： A01H1/00 ,  C12N15/32 ,  C12N1/21 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N9/0083 ,  C12N9/1085 ,  C12N15/52 ,  C12P23/00

    摘要： The present invention provides methods to engineer microorganisms for the production of C30-aldehyde carotenoids. Specifically, various combinations of crtM, sqs, crtN and crtN2 genes from Staphylococcus aureus and Methylomonas sp. 16a can be co-expressed in transformant hosts, leading to the production of diaponeurosporene monoaldehyde, diapocarotene monoaldehyde, and/or diapocarotene dialdehyde. In a preferred embodiment, the genetically engineered pathway is introduced into a strain of Escherichia coli that has been engineered for the expression of carotenoids, and the C30-carotenoid product is diapocarotene dialdehyde.

    摘要翻译： 本发明提供了工程微生物用于生产C 30 N-醛类胡萝卜素的方法。 具体来说，来自金黄色葡萄球菌和Methylomonas sp。的crtM，sqs，crtN和crtN2基因的各种组合。 16a可以在转化体宿主中共表达，导致产生二头孢噻吩一醛，二卡非罗芬单醛和/或二卡必康二醛。 在优选的实施方案中，将经遗传工程改造的途径引入已被改造用于表达类胡萝卜素的大肠杆菌菌株中，并且C 30-30-类胡萝卜素产物是二卡必康二醛。

    Genes encoding carotenoid compounds
    6.
    发明授权
    Genes encoding carotenoid compounds 失效
    编码类胡萝卜素的基因

    公开(公告)号：US07064196B2

    公开(公告)日：2006-06-20

    申请号：US10808979

    申请日：2004-03-25

    申请人： Qiong Cheng ,  Luan Tao

    发明人： Qiong Cheng ,  Luan Tao

    IPC分类号： C12N15/31 ,  C12N15/54 ,  C12N15/55

    CPC分类号： C12N9/001 ,  C12N9/00 ,  C12N9/0004 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12P23/00

    摘要： Genes have been isolated from strain DC260, a member of the Enterobacteriaceae family, encoding geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), phytoene synthase (CrtB), phytoene desaturase (CrtI), lycopene cyclase (CrtY), β-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl transferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译： 基因已经从肠杆菌科家族的成员DC260中分离出来，编码香叶基香叶基焦磷酸（GGPP）合成酶（CrtE），八氢番茄红素合酶（CrtB），八氢番茄红素去饱和酶（CrtI），番茄红素环化酶（CrtY），β-胡萝卜素羟化酶 ）和玉米黄质葡萄糖基转移酶（CrtX）活性。 这些基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。 公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

    Genes encoding carotenoid compounds
    7.
    发明授权
    Genes encoding carotenoid compounds 失效
    编码类胡萝卜素的基因

    公开(公告)号：US06929928B2

    公开(公告)日：2005-08-16

    申请号：US10808807

    申请日：2004-03-24

    申请人： Qiong Cheng ,  Natalia Sedkova ,  Luan Tao

    发明人： Qiong Cheng ,  Natalia Sedkova ,  Luan Tao

    IPC分类号： C07H21/04 ,  C12N20060101 ,  C12N1/14 ,  C12N1/20 ,  C12N1/21 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12N15/82 ,  C12P5/00 ,  C12P5/02 ,  C12P7/26 ,  C12P23/00

    CPC分类号： C12N9/00 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/825 ,  C12P23/00 ,  C12Y205/01029 ,  Y02E50/343

    摘要： A unique carotenogenic biosynthetic gene cluster has been isolated from Panteoa agglomerans strain DC404, wherein the genetic organization of the cluster is crtE-idi-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译： 已经从成团Panthena菌株DC404中分离出独特的致病基因生物合成基因簇，其中簇的遗传组织为crtE-idi-crtY-crtI-crtB-crtZ。 包含在该簇内的基因编码er牛儿基焦磷酸（GGPP）合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（CrtI），八氢番茄红素合酶（CrtB）和β-胡萝卜素羟化酶（CrtZ） 。 基因簇，基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。 公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

    Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number
    9.
    发明授权
    Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number 失效
    具有改变的质粒拷贝数的基于pBBR1的质粒突变体衍生物

    公开(公告)号：US07247467B2

    公开(公告)日：2007-07-24

    申请号：US10940052

    申请日：2004-09-14

    申请人： Qiong Cheng ,  Luan Tao

    发明人： Qiong Cheng ,  Luan Tao

    IPC分类号： C12N1/21

    CPC分类号： C12N15/70 ,  C12N15/74

    摘要： The present invention describes a mutant plasmid replication control region having the ability to convey a phenotype of altered plasmid copy number to the plasmid on which it resides. The mutant replication control region is based on a similar region isolated from the pBBR1 plasmid family. Plasmids containing this replication control region cannot be classed as belonging to any known incompatibility group and thus may co-exist with a broad range of other plasmids in a single host.

    摘要翻译： 本发明描述了具有将改变的质粒拷贝数表型转移到其所在质粒上的突变质粒复制控制区。 突变体复制控制区基于与pBBR1质粒家族分离的相似区域。 含有该复制控制区的质粒不能归类为任何已知的不相容性基团，因此可以与单个宿主中的宽范围的其他质粒共存。