公开(公告)号：US20230383440A1

公开(公告)日：2023-11-30

申请号：US18231460

申请日：2023-08-08

Applicant: 10x Genomics, Inc.

Inventor： Jason Bell ,  Francesca Meschi ,  Geoffrey McDermott ,  Michael Schnall-Levin ,  Xinying Zheng

IPC: C40B30/04 ,  C07K14/74 ,  C12N15/85 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

CPC classification number: C40B30/04 ,  C07K14/70539 ,  C12N15/85 ,  G01N33/532 ,  G01N33/54306 ,  G01N33/548 ,  C12N15/1037 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/505 ,  G01N33/5308 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/5304 ,  G01N33/5306 ,  C12N15/1055 ,  C12N15/1065 ,  G01N33/5032 ,  G01N33/54366 ,  G01N33/56977 ,  C12Q2537/164 ,  C12Q2565/1015 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12N2310/20 ,  C40B70/00

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes.

公开(公告)号：US20220145370A1

公开(公告)日：2022-05-12

申请号：US17480724

申请日：2021-09-21

Applicant: 10x Genomics, Inc.

Inventor： Luigi Jhon Alvarado Martinez ,  Eswar Prasad Ramachandran Iyer ,  Jason Bell

IPC: C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874

Abstract: Provided herein are methods, compositions, and systems for processing nucleic acids from individual cells or cell populations. Cells are co-partitioned and processed, allowing for analysis of a variety of types of RNA from cells while minimizing ribosomal RNA species. The present invention enables high-throughput measurement of full length RNA from single cells with a streamlined workflow.

公开(公告)号：US20210270818A1

公开(公告)日：2021-09-02

申请号：US17229859

申请日：2021-04-13

Applicant: 10X Genomics, Inc.

Inventor： Jason Bell ,  Francesca Meschi ,  Geoffrey McDermott ,  Michael Schnall-Levin ,  Xinying Zheng

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20190367966A1

公开(公告)日：2019-12-05

申请号：US16375102

申请日：2019-04-04

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC: C12Q1/6806 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6804 ,  G01N33/50 ,  C12N15/10

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.