公开(公告)号：US20210040434A1

公开(公告)日：2021-02-11

申请号：US16977244

申请日：2019-05-02

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Alexi J. Young ,  Evan D. Brutinel ,  Patrick A. Mach ,  Adam J. Stanenas

IPC: C12M1/34 ,  C12M1/00 ,  C12M1/12 ,  C12Q1/04

Abstract: A selective microbial detection device and methods of use are provided. The device includes a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. The microbial detection device also includes an effective amount of a dry nutrient disposed in the first compartment, which contains sodium lauryl sulfate in an amount of 1.75 milligrams or greater per twelve square inches of the first wall portion. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A sealable sample port provides access to deposit a liquid into the first compartment.

公开(公告)号：US20180243737A1

公开(公告)日：2018-08-30

申请号：US15962391

申请日：2018-04-25

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Stephen B. Roscoe ,  Myungchan Kang ,  Evan D. Brutinel ,  Jesse D. Miller ,  Renee K. Cline ,  Minghua Dai ,  Hsi-Chou Liu

IPC: B01L3/00 ,  G01N33/53

CPC classification number: B01L3/5029 ,  B01L3/502 ,  B01L2200/16 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/0478 ,  G01N33/5304

Abstract: Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.

公开(公告)号：US11851643B2

公开(公告)日：2023-12-26

申请号：US16977244

申请日：2019-05-02

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Alexi J. Young ,  Evan D. Brutinel ,  Patrick A. Mach ,  Adam J. Stanenas

IPC: C12M1/34 ,  C12M1/00 ,  C12M1/12 ,  C12Q1/04

CPC classification number: C12M41/46 ,  C12M23/14 ,  C12M23/20 ,  C12M25/02 ,  C12M41/14 ,  C12Q1/04

Abstract: A selective microbial detection device and methods of use are provided. The device includes a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. The microbial detection device also includes an effective amount of a dry nutrient disposed in the first compartment, which contains sodium lauryl sulfate in an amount of 1.75 milligrams or greater per twelve square inches of the first wall portion. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A sealable sample port provides access to deposit a liquid into the first compartment.

公开(公告)号：US10808215B2

公开(公告)日：2020-10-20

申请号：US15503481

申请日：2015-08-19

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Evan D. Brutinel ,  Jason W. Bjork ,  Adam J. Stanenas

IPC: C12M1/14 ,  C12Q1/04 ,  C12M1/26 ,  C12M1/12 ,  C12M1/00 ,  C12M1/34

Abstract: The present disclosure provides a culture device for enumerating colonies of sulfate-reducing microorganisms. The device includes a body having a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween. The growth compartment has a perimeter and an opening. A portion of the perimeter is defined by a waterproof seal. The portion can include >50% of the perimeter. Disposed in the growth compartment are a dry cold water-soluble gelling agent, a dry culture medium selected to facilitate growth of a sulfate-reducing bacterium or indicator reagent for detecting hydrogen sulfide production by a sulfate-reducing bacterium, and a dry first oxygen-scavenging reagent.

公开(公告)号：US10723992B2

公开(公告)日：2020-07-28

申请号：US15569451

申请日：2016-04-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Jason W. Bjork ,  Evan D. Brutinel ,  Adam J. Stanenas

IPC: C12M1/34 ,  C12M1/12 ,  C12M1/00

Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer is adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and an effective amount of a dry carbon dioxide-generating reagent are adhered to the at least one adhesive layer. The carbon dioxide-generating reagent consists essentially of particles having a diameter of less than 106 microns.

公开(公告)号：US20200056219A1

公开(公告)日：2020-02-20

申请号：US16461397

申请日：2017-12-22

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Alexi J. Young ,  Evan D. Brutinel

IPC: C12Q1/04 ,  C12M1/12

Abstract: Devices for microbial detection of microorganisms are provided including a body member including a substrate having a first major surface and a second major surface. The device further includes a substantially dry, first microbial growth nutrient composition disposed on a portion of the first major surface, a first adhesive composition adhered to the first microbial growth nutrient composition, and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition. The device also includes a cover sheet attached to the body member, where the cover sheet includes a first major surface facing the body member. Devices including a water-proof pouch are also provided. Methods for detecting and enumerating at least one microorganism in a sample are additionally provided, using the devices.

公开(公告)号：US20200056136A1

公开(公告)日：2020-02-20

申请号：US16461392

申请日：2017-12-22

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Evan D. Brutinel ,  Alexi J. Young

IPC: C12M1/12 ,  C12M1/00 ,  B01L3/00 ,  C12Q1/06

Abstract: Devices for microbial detection of microorganisms are provided including a body member including a substrate having a first major surface and a second major surface. The device further includes a first adhesive composition adhered to a portion of the first major surface. A number of particles of a substantially dry first microbial growth nutrient composition are distributed in the first adhesive composition, and a cold-water-soluble first hydrogel-forming composition is adhered to the first adhesive composition. The device also includes a cover sheet attached to the body member, where the cover sheet includes a first major surface facing the body member. Devices including a water-proof pouch, further comprising a porous membrane filter are also provided. Methods for detecting and enumerating at least one microorganism in a sample using the devices are additionally provided.

公开(公告)号：US10344253B2

公开(公告)日：2019-07-09

申请号：US15519944

申请日：2015-11-16

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Evan D. Brutinel ,  Alexi J. Young ,  Adam J. Stanenas

IPC: C12M1/12 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/04 ,  A61L2/08 ,  C12Q1/06

Abstract: Devices for the propagation or storage of microorganisms are provided including a first layer that has a first portion of a surface of the first layer, to which a first water-swellable gelling agent comprising a first clay is affixed. The devices further include a second layer that is separable from the first layer and has a first portion of a surface of the second layer, to which a second water-swellable gelling agent is affixed. Methods for detecting and enumerating at least one microorganism in a sample are provided. The methods include providing a device, separating the first layer from the second layer, adding an aliquot of a sample containing at least one microorganism onto the first or second water-swellable gelling agent to form an inoculated device, laminating the first layer back to the second layer, and incubating the inoculated device. Kits and methods of making the devices are also provided.

公开(公告)号：US20190030938A1

公开(公告)日：2019-01-31

申请号：US16063472

申请日：2016-12-12

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Eric A. Vandre ,  Matthew S. Stay ,  Matthew R.D. Smith ,  Ann M. Gilman ,  Shawn C. Dodds ,  Jon E. Dable ,  Evan D. Brutinel

IPC: B41M1/12 ,  B41M1/22 ,  B41M1/26

CPC classification number: B41M1/125 ,  B41M1/12 ,  B41M1/22 ,  B41M1/26

Abstract: Methods for using a hollow, rotating stencil roll to deposit flowable dry powder particles onto a moving substrate and to attach the particles to the substrate.

公开(公告)号：US20190003930A1

公开(公告)日：2019-01-03

申请号：US16064271

申请日：2016-12-21

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Ruby L. Byrne ,  Evan D. Brutinel ,  Michael A. Haase

IPC: G01N1/18 ,  B01L3/00

Abstract: Cartridges for sample partitioning and methods of making and using the cartridges are provided. The cartridges include a pouch to receive a mated stem-well film and a sealing film covered by a release liner. The stem film and the sealing film can be respectively separated from the well film and sealing film simultaneously by pulling a pull tab.