公开(公告)号：US20210108257A1

公开(公告)日：2021-04-15

申请号：US16468846

申请日：2017-12-15

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green

IPC: C12Q1/6841 ,  G01N33/53

Abstract: Provided herein are imaging probes and systems and methods employing such imaging probes for real-time, label-free, multiplexed imaging of RNAs in living cells. More particularly, aptamer-based sensors (“aptasensors”) and molecular fuses comprising multiple aptasensors are genetically encoded imaging probes comprising RNA-target binding sequence and an intramolecular reconfiguration sequence. The probe is configured such that binding of a RNA target by the RNA-target binding sequence triggers the intramolecular reconfiguration sequence to reconfigure such that an optically detectable output is generated by the probe.

公开(公告)号：US20200071777A1

公开(公告)日：2020-03-05

申请号：US16349752

申请日：2017-11-15

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6897 ,  C12Q1/6858

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US20250075256A1

公开(公告)日：2025-03-06

申请号：US18830513

申请日：2024-09-10

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： Alexander Green ,  Dana Braff ,  Melissa Takahashi ,  Keith Pardee ,  James J. Collins ,  Guillaume Lambert ,  Thomas Ferrante

IPC: C12Q1/6827 ,  C12Q1/6816 ,  C12Q1/70

Abstract: Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.

公开(公告)号：US12116623B2

公开(公告)日：2024-10-15

申请号：US17849250

申请日：2022-06-24

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： Alexander Green ,  Dana Braff ,  Melissa Takahashi ,  Keith Pardee ,  James J. Collins ,  Guillaume Lambert ,  Thomas Ferrante

IPC: C12Q1/6827 ,  C12Q1/6816 ,  C12Q1/70

CPC classification number: C12Q1/6827 ,  C12Q1/6816 ,  C12Q1/701

Abstract: Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.

公开(公告)号：US20240200069A1

公开(公告)日：2024-06-20

申请号：US18403466

申请日：2024-01-03

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Yu Zhou

IPC: C12N15/113 ,  C12N15/67 ,  H03K19/20

CPC classification number: C12N15/113 ,  C12N15/67 ,  H03K19/20 ,  C12N2310/122 ,  C12N2310/531

Abstract: Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.

公开(公告)号：US20240018608A1

公开(公告)日：2024-01-18

申请号：US18465077

申请日：2023-09-11

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green ,  Duo Ma

IPC: C12Q1/70 ,  C12Q1/6816

CPC classification number: C12Q1/701 ,  C12Q1/6816

Abstract: Provided herein are synthetic nucleic acid molecules known as loop-mediated riboregulators that have single-nucleotide polymorphism (SNP) sensitivity and ultralow OFF state signal levels. Loop-mediated riboregulators can activate or repress gene expression in response to trigger RNAs bearing completely arbitrary sequences. Also provided herein are methods of using such synthetic nucleic acid molecules for detecting the presence or absence of a particular target RNA in, for example, a biological sample.

公开(公告)号：US11802318B2

公开(公告)日：2023-10-31

申请号：US16323103

申请日：2017-08-04

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Duo Ma

IPC: C12Q1/6897 ,  C12Q1/70 ,  C12N15/113 ,  C12Q1/68 ,  C12N15/67 ,  C12N15/63 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/689

CPC classification number: C12Q1/6897 ,  C12N15/113 ,  C12N15/63 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/6809 ,  C12Q1/689 ,  C12Q1/6816 ,  C12Q1/70 ,  C12N2310/531

Abstract: Provided herein are synthetic nucleic acid molecules known as loop-mediated riboregulators that have single-nucleotide polymorphism (SNP) sensitivity and ultralow OFF state signal levels. Loop-mediated riboregulators can activate or repress gene expression in response to trigger RNAs bearing completely arbitrary sequences. Also provided herein are methods of using such synthetic nucleic acid molecules for detecting the presence or absence of a particular target RNA in, for example, a biological sample.

公开(公告)号：US11788125B2

公开(公告)日：2023-10-17

申请号：US17324944

申请日：2021-05-19

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green

IPC: C07H21/02 ,  C12Q1/6841 ,  C12N15/115 ,  G01N33/53

CPC classification number: C12Q1/6841 ,  C12N15/115 ,  G01N33/5308 ,  C12N2310/16 ,  C12N2310/531 ,  C12Q2525/205 ,  C12Q2525/301

Abstract: Provided herein are imaging probes and systems and methods employing such imaging probes for real-time, label-free, multiplexed imaging of RNAs in living cells. More particularly, aptamer-based sensors (“aptasensors”) and molecular fuses comprising multiple aptasensors are genetically encoded imaging probes comprising RNA-target binding sequence and an intramolecular reconfiguration sequence. The probe is configured such that binding of a RNA target by the RNA-target binding sequence triggers the intramolecular reconfiguration sequence to reconfigure such that an optically detectable output is generated by the probe.

公开(公告)号：US11773430B2

公开(公告)日：2023-10-03

申请号：US16342722

申请日：2017-10-17

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Duo Ma ,  Anli Tang

IPC: C12Q1/6825 ,  C12Q1/6816

CPC classification number: C12Q1/6825 ,  C12Q1/6816 ,  Y02A50/30 ,  C12Q1/6825 ,  C12Q2525/205 ,  C12Q1/6816 ,  C12Q2525/205 ,  C12Q2565/625

Abstract: Methods and compositions for detecting the presence of a pathogen are described. In particular, this document provides a method of detecting a pathogen-associated nucleic acid in a biological sample of a subject, where the method comprises using one or more unimolecular aptamer-based sensors comprising an aptamer-fluorophore complex and an amplification step to detect the pathogen-associated nucleic acid. Methods specific for detecting the presence of malaria and other mosquito-borne virus infections are also provided.

公开(公告)号：US11371081B2

公开(公告)日：2022-06-28

申请号：US16303937

申请日：2017-05-25

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： Alexander Green ,  Dana Braff ,  Melissa K. Takahashi ,  Keith Pardee ,  James J. Collins ,  Guillaume Lambert ,  Thomas Ferrante

IPC: C12Q1/6827 ,  C12Q1/6816 ,  C12Q1/70

Abstract: Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.