公开(公告)号：US20200071777A1

公开(公告)日：2020-03-05

申请号：US16349752

申请日：2017-11-15

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6897 ,  C12Q1/6858

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US20180216102A1

公开(公告)日：2018-08-02

申请号：US15937371

申请日：2018-03-27

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joseph Blattman ,  Hao Yan ,  Louis Schoettle ,  Xixi Wei

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1062 ,  B82Y5/00 ,  B82Y15/00 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6881 ,  C12Q2600/16 ,  C12Q2563/155

Abstract: Methods for construction of DNA origami nanostructures, as well as for binding, isolation, linking, and deep sequencing information, such as both of TCR alpha and beta CDR3 mRNA, from individual cells within a mixed population of cells without the need for single cell sorting (FIG. 1).