利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

31 条记录 (耗时 0.036 秒)

    I Sce I-induced gene replacement and gene conversion in embryonic stem cells
    22.
    发明授权
    I Sce I-induced gene replacement and gene conversion in embryonic stem cells 失效
    I Sce I诱导的胚胎干细胞中的基因替换和基因转化

    公开(公告)号：US5830729A

    公开(公告)日：1998-11-03

    申请号：US693948

    申请日：1996-08-07

    申请人： Frederic Jaisser ,  Michel Cohen-Tannoudji ,  Sylvie Robine ,  Andre Choulika ,  Daniel Louvard ,  Charles Babinet

    发明人： Frederic Jaisser ,  Michel Cohen-Tannoudji ,  Sylvie Robine ,  Andre Choulika ,  Daniel Louvard ,  Charles Babinet

    IPC分类号： C07K14/47 ,  C12N15/36 ,  C12N15/00

    CPC分类号： C07K14/4735

    摘要： This invention relates to a method of constructing a villin gene hybrid by inserting an I-Sce I restriction site next to or within a gene or cDNA encoding a villin protein. The insertion site of the I-Sce I restriction site is chosen as to provide a first downstream part and a second upstream part from the site, containing at least twelve nucleotides of the gene or cDNA encoding the villin protein. Furthermore, the insertion of the restriction permits a high frequency of homologous recombination events. The villin gene hybrid may be used to transfect eukaryotic cells, and particularly, embryonic stem cells.

    摘要翻译： 本发明涉及一种通过在编码绒毛蛋白的基因或cDNA之前或之内插入I-Sce I限制性位点来构建绒毛蛋白基因杂交体的方法。 选择I-Sce I限制性位点的插入位点，从该位点提供第一个下游部分和第二个上游部分，其中含有至少12个核苷酸的基因或编码绒毛蛋白的cDNA。 此外，插入限制允许高频率的同源重组事件。 绒毛蛋白基因杂交体可用于转染真核细胞，特别是胚胎干细胞。

    HYBRID AND SINGLE CHAIN MEGANUCLEASES AND USE THEREOF
    24.
    发明申请
    HYBRID AND SINGLE CHAIN MEGANUCLEASES AND USE THEREOF 审中-公开
    混合和单链MEGANUCLEASES及其使用

    公开(公告)号：US20100151556A1

    公开(公告)日：2010-06-17

    申请号：US12482124

    申请日：2009-06-10

    申请人： Sylvain Arnould ,  Patrick Chames ,  Andre Choulika ,  Jean-Charles Epinat ,  Sylvestre Grizot ,  Emmanuel Lacroix

    发明人： Sylvain Arnould ,  Patrick Chames ,  Andre Choulika ,  Jean-Charles Epinat ,  Sylvestre Grizot ,  Emmanuel Lacroix

    IPC分类号： C12N7/00 ,  C12N9/16 ,  C07H21/04 ,  C12N15/63

    CPC分类号： C12N9/22

    摘要： This patent application relates to hybrid and/or single-chain rare-cutting endonucleases, called meganucleases, which recognize and cleave a specific nucleotide sequence, to polynucleotide sequences encoding for said rare-cutting endonucleases, to a vector comprising one of said polynucleotide sequences, to a cell or animal comprising one of said polynucleotide sequences or said rare-cutting endonucleases, to a process for producing one of said rare-cutting endonucleases and any use of the disclosed products and methods. More particularly, this invention contemplates any use of such rare-cutting endonuclease for genetic engineering and gene therapy.

    摘要翻译： 该专利申请涉及称为大范围核酸酶的杂交和/或单链稀释内切核酸酶，其将编码所述稀少切割内切核酸酶的多核苷酸序列识别并切割特定核苷酸序列至包含所述多核苷酸序列之一的载体， 涉及包含所述多核苷酸序列之一或所述稀少切割内切核酸酶的细胞或动物，涉及生产所述稀有切割内切核酸酶之一的方法和所公开产品和方法的任何用途。 更具体地，本发明考虑了这种稀释内切核酸酶用于遗传工程和基因治疗的任何用途。

    Meganuclease recombination system
    27.
    发明授权
    Meganuclease recombination system 有权
    大范围核酸酶重组体系

    公开(公告)号：US08476072B2

    公开(公告)日：2013-07-02

    申请号：US13125193

    申请日：2009-10-23

    申请人： Jean-Pierre Cabaniols ,  Andre Choulika ,  Christophe Delenda

    发明人： Jean-Pierre Cabaniols ,  Andre Choulika ,  Christophe Delenda

    IPC分类号： C12N15/87 ,  C12N15/00 ,  C12N15/10

    CPC分类号： C12N15/85 ,  C12N15/902

    摘要： The invention relates to a set of genetic constructs which allow the efficient and reproducible introduction of a specific nucleotide sequence at a fixed position in the genome by generating a double strand break at a specific position in the genome using a meganuclease and so stimulating a homologous recombination event at this locus between the genomic site and a transfected donor sequence. The present invention also relates to methods using these constructs and to these materials in the form of a kit.

    摘要翻译： 本发明涉及一组基因构建体，其允许通过使用大范围核酸酶在基因组中的特定位置处产生双链断裂并且因此刺激同源重组而在基因组中的固定位置有效且可重复地引入特定核苷酸序列 在该基因座位点和转染供体序列之间的事件。 本发明还涉及以试剂盒形式使用这些构建体和这些材料的方法。

    Hybrid and single chain meganucleases and use thereof
    28.
    发明授权
    Hybrid and single chain meganucleases and use thereof 有权
    杂交和单链大分子核酸酶及其用途

    公开(公告)号：US08206965B2

    公开(公告)日：2012-06-26

    申请号：US10388230

    申请日：2003-03-14

    申请人： Sylvain Arnould ,  Patrick Chames ,  Andre Choulika ,  Jean-Charles Epinat ,  Emmanuel Lacroix

    发明人： Sylvain Arnould ,  Patrick Chames ,  Andre Choulika ,  Jean-Charles Epinat ,  Emmanuel Lacroix

    IPC分类号： C12N9/22 ,  C12N15/55

    CPC分类号： C12N9/22 ,  C07K2319/00

    摘要： This patent application relates to hybrid and/or single-chain rare-cutting endonucleases, called meganucleases, which recognize and cleave a specific nucleotide sequence, to polynucleotide sequences encoding for said rare-cutting endonucleases, to a vector comprising one of said polynucleotide sequences, to a cell or animal comprising one of said polynucleotide sequences or said rare-cutting endonucleases, to a process for producing one of said rare-cutting endonucleases and any use of the disclosed products and methods. More particularly, this invention contemplates any use of such rare-cutting endonuclease for genetic engineering and gene therapy.

    摘要翻译： 该专利申请涉及称为大范围核酸酶的杂交和/或单链稀释内切核酸酶，其将编码所述稀少切割内切核酸酶的多核苷酸序列识别并切割特定核苷酸序列至包含所述多核苷酸序列之一的载体， 涉及包含所述多核苷酸序列之一或所述稀少切割内切核酸酶的细胞或动物，涉及生产所述稀有切割内切核酸酶之一的方法和所公开产品和方法的任何用途。 更具体地，本发明考虑了这种稀释内切核酸酶用于遗传工程和基因治疗的任何用途。

    Sequence of natural or synthetic retroelements enabling nucleotide sequence insertion into a eukaryotic cell
    30.
    发明申请
    Sequence of natural or synthetic retroelements enabling nucleotide sequence insertion into a eukaryotic cell 审中-公开
    使核苷酸序列插入真核细胞的天然或合成逆转录酶的序列

    公开(公告)号：US20060269522A1

    公开(公告)日：2006-11-30

    申请号：US11431075

    申请日：2006-05-10

    申请人： Andre Choulika ,  Jean-Francois Nicolas

    发明人： Andre Choulika ,  Jean-Francois Nicolas

    IPC分类号： A61K48/00 ,  C12N15/867

    CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C12N2740/13043 ,  C12N2800/30

    摘要： A retroviral vector that eliminates cis-acting retroviral elements, which are not useful to and may cause problems for, an integrated provirus utilizes a recombination system, such as the bacteriophage P1 cre-lox recombination system. A gene of interest and a loxP site are inserted into a long terminal repeat, which is then duplicated. The enzyme cre is inserted between the long terminal repeats and combines them. The structure of the resulting provirus in the host genome is that of a single, long terminal repeat carrying a single copy of the gene of interest. Following infection, the retrovirus produces proviral structures having only a single long terminal repeat.

    摘要翻译： 消除顺式作用的逆转录病毒元件的逆转录病毒载体，其不能用于整合的原病毒并且可能引起问题，其利用重组系统，例如噬菌体P1 cre-lox重组系统。 将感兴趣的基因和loxP位点插入长末端重复序列，然后将其重复。 将酶标记插入到长末端重复序列之间并将其组合。 宿主基因组中所得到的原病毒的结构是携带目的基因的单拷贝的单个长末端重复序列的结构。 感染后，逆转录病毒产生仅具有单个长末端重复序列的前病毒结构。