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    Sequence of natural or synthetic retroelements enabling nucleotide sequence insertion into a eukaryotic cell
    7.
    发明申请
    Sequence of natural or synthetic retroelements enabling nucleotide sequence insertion into a eukaryotic cell 审中-公开
    使核苷酸序列插入真核细胞的天然或合成逆转录酶的序列

    公开(公告)号：US20060269522A1

    公开(公告)日：2006-11-30

    申请号：US11431075

    申请日：2006-05-10

    申请人： Andre Choulika ,  Jean-Francois Nicolas

    发明人： Andre Choulika ,  Jean-Francois Nicolas

    IPC分类号： A61K48/00 ,  C12N15/867

    CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C12N2740/13043 ,  C12N2800/30

    摘要： A retroviral vector that eliminates cis-acting retroviral elements, which are not useful to and may cause problems for, an integrated provirus utilizes a recombination system, such as the bacteriophage P1 cre-lox recombination system. A gene of interest and a loxP site are inserted into a long terminal repeat, which is then duplicated. The enzyme cre is inserted between the long terminal repeats and combines them. The structure of the resulting provirus in the host genome is that of a single, long terminal repeat carrying a single copy of the gene of interest. Following infection, the retrovirus produces proviral structures having only a single long terminal repeat.

    摘要翻译： 消除顺式作用的逆转录病毒元件的逆转录病毒载体，其不能用于整合的原病毒并且可能引起问题，其利用重组系统，例如噬菌体P1 cre-lox重组系统。 将感兴趣的基因和loxP位点插入长末端重复序列，然后将其重复。 将酶标记插入到长末端重复序列之间并将其组合。 宿主基因组中所得到的原病毒的结构是携带目的基因的单拷贝的单个长末端重复序列的结构。 感染后，逆转录病毒产生仅具有单个长末端重复序列的前病毒结构。